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首页> 外文期刊>Environmental Science & Technology >Internal Concentration and Time Are Important Modifiers of Toxicity: The Case of Chlorpyrifos on Caenorhabditis elegans
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Internal Concentration and Time Are Important Modifiers of Toxicity: The Case of Chlorpyrifos on Caenorhabditis elegans

机译：内部浓度和时间是毒性的重要调节剂：毒死rif在秀丽隐杆线虫的情况下

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摘要

The internal concentration of chemicals in exposed organisms changes over time due to absorption, distribution, metabolism, and excretion processes since chemicals are taken up from the environment. Internal concentration and time are very important modifiers of toxicity when biomarkers are used to evaluate the potential hazards and risks of environmental pollutants. In this study, the responses of molecular biomarkers, and the fate of chemicals in the body, were comprehensively investigated to determine cause-and-effect relationships over time. Chlorpyrifos (CP) was selected as a model chemical, and Caenorhabditis elegans was exposed to CP for 4 h using the passive dosing method. Worms were then monitored in fresh medium during a 48-h recovery regime. The mRNA expression of genes related to CYP metabolism (cyp35a2 and cyp35a3) increased during the constant exposure phase. The body residue of CP decreased once it reached a peak level during the early stage of exposure, indicating that the initial uptake of CP rapidly induced biotransformation with the synthesis of new CYP metabolic proteins. The residual chlorpyrifos-oxon concentration, an acetyicholinesterase (AChE) inhibitor, continuously increased even after the recovery regime started. These delayed toxicokinetics seem to be important for the extension of AChE inhibition for up to 9 h after the start of the recovery regime. Comprehensive investigation into the molecular initiation events and changes in the internal concentrations of chemical species provide insight into response causality within the framework of an adverse outcome pathway.
机译：由于化学品是从环境中吸收的，因此由于吸收，分布，代谢和排泄过程，暴露有机体中化学品的内部浓度会随时间而变化。当使用生物标志物评估环境污染物的潜在危害和风险时，内部浓度和时间是毒性的重要调节剂。在这项研究中，对分子生物标志物的反应以及体内化学物质的命运进行了全面研究，以确定随时间变化的因果关系。选择毒死rif（CP）作为模型化学品，使用被动加药方法将秀丽隐杆线虫暴露于CP 4 h。然后在48小时的恢复过程中在新鲜培养基中监测蠕虫。在恒定暴露阶段，与CYP代谢相关的基因（cyp35a2和cyp35a3）的mRNA表达增加。 CP的身体残留一旦在暴露的早期达到峰值，就会减少，这表明CP的最初摄取迅速诱导了生物转化并合成了新的CYP代谢蛋白。残留的毒死rif-oxon浓度（一种乙酰胆碱酯酶（AChE）抑制剂）即使在恢复方案开始后仍持续增加。在恢复方案开始后，这些延迟的毒代动力学似乎对于延长AChE抑制长达9小时很重要。对分子引发事件和化学物种内部浓度变化的全面研究提供了对不良结果途径框架内反应因果关系的深入了解。

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  • 来源
    《Environmental Science & Technology》 |2016年第17期|9689-9696|共8页
  • 作者

    Ji-Yeon Roh; Hyun-Jeoung Lee; Jung-Hwan Kwon;

  • 作者单位

    Division of Environmental Science and Ecological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea;

    Division of Environmental Science and Ecological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea;

    Division of Environmental Science and Ecological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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