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首页> 外文期刊>Environmental Science & Technology >1,2-Dichloroethane Exposure Alters the Population Structure, Metabolism, and Kinetics of a Trichloroethene-Dechlorinating Dehalococcoides mccartyi Consortium
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1,2-Dichloroethane Exposure Alters the Population Structure, Metabolism, and Kinetics of a Trichloroethene-Dechlorinating Dehalococcoides mccartyi Consortium

机译：1,2-二氯乙烷的暴露改变了三氯乙烯-脱氯Dehalococcoides麦卡蒂财团的人口结构，代谢和动力学。

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摘要

Bioremediation of groundwater contaminated with chlorinated aliphatic hydrocarbons such as perchloroethene and trichloroethene can result in the accumulation of the undesirable intermediate vinyl chloride. Such accumulation can either be due to the absence of specific vinyl chloride respiring Dehalococcoides mccartyi or to the inhibition of such strains by the metabolism of other microorganisms. The fitness of vinyl chloride respiring Dehalococcoides mccartyi subpopulatjons is particularly uncertain in the presence of chloroethene/chloroethane cocontaminant mixtures, which are commonly found in contaminated ground-water. Therefore, we investigated the structure of Dehalococcoides populations in a continuously fed reactor system under changing chloroethene/ethane influent conditions. We observed that increasing the influent ratio of 1,2-dichloroethane to trichloroethene was associated with ecological selection of a tceA-containing Dehalococcoides population relative to a vcrA-containing Dehalococcoides population. Although both vinyl chloride and 1,2-dichloroethane could be simultaneously transformed to ethene, prolonged exposure to 1,2-dichloroethane diminished the vinyl chloride transforming capacity of the culture. Kinetic tests revealed that dechlorination of 1,2-dichloroethane by the consortium was strongly inhibited by cis-dichloroethene but not vinyl chloride. Native polyacrylamide gel electrophoresis and mass spectrometry revealed that a trichloroethene reductive dehalogenase (TceA) homologue was the most consistently expressed of four detectable reductive dehalogenases during 1,2-dichloroethane exposure, suggesting that it catalyzes the reductive dihaloelimination of 1,2-dichloroethane to ethene.
机译：对被氯代脂族烃（如全氯乙烯和三氯乙烯）污染的地下水进行生物修复可能导致不良的中间体氯乙烯的积累。这种积累可能是由于缺乏呼吸氯甲烷的特异氯乙烯，或者是由于其他微生物的新陈代谢而抑制了这些菌株。在氯乙烯/氯乙烷共污染物混合物的存在下，呼吸氯乙烯的Dehalococcoides mccartyi亚种群的适应性尤其不确定，这通常在受污染的地下水中发现。因此，我们研究了在不断变化的氯乙烯/乙烷进水条件下，连续进料反应器系统中脱卤代球菌种群的结构。我们观察到，相对于含有vcrA的Dehalococcoides种群，增加1,2-二氯乙烷与三氯乙烯的进水比与生态选择含tceA的Dehalococcoides种群有关。尽管氯乙烯和1,2-二氯乙烷均可同时转化为乙烯，但长时间暴露于1,2-二氯乙烷会使培养物中氯乙烯的转化能力降低。动力学测试表明，财团对1,2-二氯乙烷的脱氯作用受到顺-二氯乙烯的强烈抑制，但不受氯乙烯的抑制。天然聚丙烯酰胺凝胶电泳和质谱分析表明，在1,2-二氯乙烷暴露期间，三氯乙烯还原脱卤酶（TceA）同源性是四种可检测到的还原脱卤酶中最一致的表达，表明它催化了1,2-二氯乙烷还原成乙烯的二卤化消除作用。 。

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  • 来源
    《Environmental Science & Technology》 |2016年第22期|12187-12196|共10页
  • 作者

    Koshlan Mayer-Blackwell; Maeva Fincker; Olivia Molenda; Benjamin Callahan; Holly Sewell; Susan Holmes; Elizabeth A. Edwards; Alfred M. Spormann;

  • 作者单位

    Civil and Environmental Engineering, Stanford University, Stanford,California 94305, United States;

    Civil and Environmental Engineering, Stanford University, Stanford,California 94305, United States;

    Chemical Engineering & Applied Chemistry, University of Toronto, Toronto, Ontario M5S 3E5, Canada;

    Department of Statistics, Stanford University, Stanford,California 94305, United States;

    Civil and Environmental Engineering, Stanford University, Stanford,California 94305, United States;

    Department of Statistics, Stanford University, Stanford,California 94305, United States;

    Chemical Engineering & Applied Chemistry, University of Toronto, Toronto, Ontario M5S 3E5, Canada,Cell and Systems Biology, University of Toronto, Toronto, Ontario M5S 3E5, Canada;

    Civil and Environmental Engineering, Stanford University, Stanford,California 94305, United States,Chemical Engineering, Stanford University, Stanford,California 94305, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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