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首页> 外文期刊>Environmental Science & Technology >Copper Tolerance Mechanisms of Mesorhizobium amorphae and Its Role in Aiding Phytostabilization by Robinia pseudoacacia in Copper Contaminated Soil
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Copper Tolerance Mechanisms of Mesorhizobium amorphae and Its Role in Aiding Phytostabilization by Robinia pseudoacacia in Copper Contaminated Soil

机译：铜污染土壤中根瘤菌的铜耐受机制及其在植物稳定中的作用

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摘要

The legume-rhizpbium symbiosis has been proposed as an important system for phytoremediation of heavy metal contaminated soils due to its beneficial activity of symbiotic nitrogen fixation. However, little is known about metal resistant mechanism of rhizobia and the role of metal resistance determinants in phytoremediation. In this study, copper resistance mechanisms were investigated for a multiple metal resistant plant growth promoting rhizobium, Mesorhizobium amorphae 186. Three categories of determinants involved in copper resistance were identified through trans-poson mutagenesis, including genes encoding a P-type ATPase (CopA), hypothetical proteins, and other proteins (a GTP-binding protein and a ribosomal protein). Among these determinants, copA played the dominant role in copper homeostasis of M. amorphae 186. Mutagenesis of a hypothetical gene lipA in mutant M_(lipA) exhibited pleiotropic phenotypes including sensitivity to copper, blocked symbiotic capacity and inhibited growth. In addition, the expression of cusB encoding part of an RND-type efflux system was induced by copper. To explore the possible role of copper resistance mechanism in phytoremediation of copper contaminated soil, the symbiotic nodulation and nitrogen fixation abilities were compared using a wild-type strain, a copA-defective mutant, and a lipA-defective mutant Results showed that a copA deletion did not affect the symbiotic capacity of rhizobia under uncontaminated condition, but the protective role of copA in symbiotic processes at high copper concentration is likely concentration-dependent. In contrast, inoculation of a lipA-defective strain led to significant decreases in the functional nodule numbers, total N content, plant biomass and leghemoglobin expression level of Robinia pseudoacacia even under conditions of uncontaminated soil. Moreover, plants inoculated with lipA-defective strain accumulated much less copper than both the wild-type strain and the copA-defective strain, suggesting an important role of a healthy symbiotic relationship between legume and rhizobia in phytostabilization.
机译：由于其对共生固氮的有益作用，豆科植物-根瘤菌共生被认为是一种植物修复重金属污染土壤的重要系统。然而，关于根瘤菌的金属抗性机理以及金属抗性决定簇在植物修复中的作用知之甚少。在这项研究中，研究了铜的抗性机制，以促进多种金属抗性植物的生长，促进了根瘤菌（Mesoorhizobium amorphae 186）的生长。通过转座子诱变鉴定了涉及铜抗性的三类决定因素，包括编码P型ATPase（CopA）的基因。 ，假设蛋白和其他蛋白（GTP结合蛋白和核糖体蛋白）。在这些决定因素中，copA在紫茉莉186的铜稳态中起主要作用。突变体M_（lipA）中假想基因lipA的诱变表现出多效性表型，包括对铜的敏感性，共生能力受阻和生长受到抑制。另外，铜诱导了RND型外排系统的cusB编码部分的表达。为了探索铜抗性机制在铜污染土壤的植物修复中的可能作用，使用野生型菌株，copA缺陷型突变体和lipA缺陷型突变体比较了共生结瘤和固氮能力。结果表明，copA缺失在未受污染的条件下，这并没有影响根瘤菌的共生能力，但是在高铜浓度下，copA在共生过程中的保护作用可能与浓度有关。相反，即使在未污染的土壤条件下，接种lipA缺陷菌株也会导致刺槐的功能性结节数，总氮含量，植物生物量和豆血红蛋白表达水平显着降低。而且，接种lipA缺陷型菌株的植物比野生型菌株和copA缺陷型菌株积累的铜少得多，这表明豆科植物和根瘤菌之间健康共生关系在植物稳定中具有重要作用。

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  • 来源
    《Environmental Science & Technology》 |2015年第4期|2328-2340|共13页
  • 作者

    Xiuli Hao; Pin Xie; Yong-Guan Zhu; Safyih Taghavi; Gehong Wei; Christopher Rensing;

  • 作者单位

    State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China,Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;

    State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China;

    Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;

    FMC Agricultural Solutions, Research Triangle Park 27709, United States;

    State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China;

    Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China,Department of Plant and Environmental Science, University of Copenhagen, Frederiksberg DK-1871, Denmark;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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