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首页> 外文期刊>Environmental Science & Technology >A Quantitative Assay for Reductive Metabolism of a Pesticide in Fish Using Electrochemistry Coupled with Liquid Chromatography Tandem Mass Spectrometry
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A Quantitative Assay for Reductive Metabolism of a Pesticide in Fish Using Electrochemistry Coupled with Liquid Chromatography Tandem Mass Spectrometry

机译：电化学结合液相色谱串联质谱法定量分析鱼中农药的还原代谢

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摘要

This is the first study to use electrochemistry to generate a nitro reduction metabolite as a standard for a liquid chromatography-mass spectrometry-based quantitative assay. This approach is further used to quantify 3-trifluoromethyl-4-nitrophenol (TFM) reductive metabolism. TFM is a widely used pesticide for the population control of sea lamprey (Petromyzon marinus), an invasive species of the Laurentian Great Lakes. Three animal models, sea lamprey, lake sturgeon (Acipenser fulvescens), and rainbow trout (Oncorhynchus mykiss), were selected to evaluate TFM reductive metabolism because they have been known to show differential susceptibilities to TFM toxicity. Amino-TFM (aTFM; 3-trifluoromethyl-4- aminophenol) was the only reductive metabolite identified through liquid chromatography-high-resolution mass spectrometry screening of liver extracts incubated with TFM and was targeted for electrochemical synthesis. After synthesis and purification, aTFM was used to develop a quantitative assay of the reductive metabolism of TFM through liquid chromatography and tandem mass spectrometry. The concentrations of aTFM were measured from TFM-treated cellular fractions, including cytosolic, nuclear, membrane, and mitochondrial protein extracts. Sea lamprey extracts produced the highest concentrations (500 ng/mL) of aTFM. In addition, sea lamprey and sturgeon cytosolic extracts showed concentrations of aTFM substantially higher than thoose of rainbow trout. However, other fractions of lake sturgeon extracts tend to show aTFM concentrations similar to those of rainbow trout but not with sea lamprey. These data suggest that the level of reductive metabolism of TFM may be associated with the sensitivities of the animals to this particular pesticide.
机译：这是第一个使用电化学法生成硝基还原代谢产物作为液相色谱-质谱联用定量分析标准的研究。该方法还用于定量3-三氟甲基-4-硝基苯酚（TFM）还原代谢。 TFM是一种广泛使用的农药，用于控制劳伦大湖的入侵物种海七sea（Petromyzon marinus）。选择了三种动物模型，分别是海鳗，湖lake鱼（Acipenser fulvescens）和虹鳟鱼（Oncorhynchus mykiss）来评估TFM还原代谢，因为已知它们对TFM毒性表现出不同的敏感性。氨基-TFM（aTFM； 3-三氟甲基-4-氨基苯酚）是通过液相色谱-高分辨率质谱筛选与TFM孵育的肝提取物而鉴定出的唯一还原性代谢产物，并靶向进行电化学合成。经过合成和纯化后，aTFM通过液相色谱和串联质谱用于定量分析TFM的还原代谢。从经TFM处理的细胞级分（包括胞质，核蛋白，膜和线粒体蛋白提取物）测量aTFM的浓度。海七rey提取物产生的aTFM浓度最高（500 ng / mL）。此外，海七rey和and鱼胞质提取物显示aTFM的浓度明显高于虹鳟鱼的含量。然而，其他部分的湖st鱼提取物往往显示出与虹鳟相似的aTFM浓度，但与海七lamp鱼没有。这些数据表明，TFM的还原代谢水平可能与动物对该特定农药的敏感性有关。

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  • 来源
    《Environmental Science & Technology》 |2015年第7期|4450-4457|共8页
  • 作者

    Ugo Bussy; Yu-Wen Chung-Davidson; Ke Li; Weiming Li;

  • 作者单位

    Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824, United States;

    Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824, United States;

    Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824, United States;

    Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824, United States,Department of Fisheries and Wildlife, Michigan State University, Room 13, Natural Resources Building, 480 Wilson Rd., East Lansing, MI 48824;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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