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首页> 外文期刊>Environmental Science & Technology >Identification of the Full 46 Cytochrome P450 (CYP) Complement and Modulation of CYP Expression in Response to Water-Accommodated Fractions of Crude Oil in the Cyclopoid Copepod Paracydopina nana
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Identification of the Full 46 Cytochrome P450 (CYP) Complement and Modulation of CYP Expression in Response to Water-Accommodated Fractions of Crude Oil in the Cyclopoid Copepod Paracydopina nana

机译：完整的46种细胞色素P450（CYP）补体的鉴定和CYP表达的调节，以响应环足类pe足类副对生菜中原油的水溶馏分

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摘要

The 46 cytochrome P450 (CYP) gene super-family was identified in the marine copepod Paracydopina nana after searching an RNA-seq database and comparing it with other copepod CYP gene families. To annotate the 46 Pn-CYP genes, a phylogenetic analysis of CYP genes was performed using a Bayesian method. Pn-CYP genes were separated into five different clans: CYP2, CYP3, CYP20, CYP26, and mitochondrial. Among these, the principal Pn-CYP genes involved in detoxification were identified by comparing them with those of the copepod Tigriopus japonicus and were examined with respect to their responses to exposure to a water-accommodated fraction (WAF) of crude oil and to the alkylated forms of two polycyctic aromatic hydrocarbons (PAHs; phenanthrene and fluorene). The expression of two Pn-CYP3027 genes (CYP3027F1 and CYP3027F2) was increased in response to WAF exposure and also was upregulated in response to the two alkyiated PAHs. In particular, Pn-CYP3027F2 showed the most notable increase in response to 80% WAF exposure. These two responsive CYP genes (Pn-CYP3027F1 and CYP3027F2) were also phylogenetically clustered into the same clade of the WAF- and alkylated PAH-spedfic CYP genes of the copepod T japonicus, suggesting that these CYP genes would be those chiefly involved in detoxification in response to WAF exposure in copepods. In this paper, we provide information on the copepod P. nana CYP gene superfamily and also speculate on its potential role in the detoxification of PAHs in marine copepods. Despite the nonlethality of WAF, Pn-CYP3027F2 was rapidly and significantly upregulated in response to WAF that may serve as a useful biomarker of 40% or higher concentration of WAF exposure. This paper will be helpful to better understand the molecular mechanistic events underlying the metabolism of environmental toxicants in copepods.
机译：通过搜索RNA-seq数据库并将其与其他co足类CYP基因家族进行比较，在海洋co足类Paracydopina nana中鉴定出46个细胞色素P450（CYP）基因超家族。为了注释46个Pn-CYP基因，使用贝叶斯方法进行了CYP基因的系统发育分析。 Pn-CYP基因被分为五个不同的家族：CYP2，CYP3，CYP20，CYP26和线粒体。其中，通过将它们与Ti足类Tigriopus japonicus的基因进行比较，鉴定了参与排毒的主要Pn-CYP基因，并检查了它们对暴露于原油的水富集馏分（WAF）和烷基化反应的反应。形式的两种多环芳烃（PAH；菲和芴）。响应于WAF暴露，两个Pn-CYP3027基因（CYP3027F1和CYP3027F2）的表达增加，并且响应于两个烷基化的PAHs也被上调。特别是，Pn-CYP3027F2在对80％WAF暴露的响应中表现出最明显的增加。这两个响应性CYP基因（Pn-CYP3027F1和CYP3027F2）也被系统地聚类到the足类T ap的WAF和烷基化PAH-spedfic CYP基因的同一进化枝中，这表明这些CYP基因将主要参与排毒co足类动物对WAF暴露的反应。在本文中，我们提供了有关the足类P. nana CYP基因超家族的信息，并推测了其在海洋PA足类中PAHs解毒中的潜在作用。尽管WAF具有非致死性，但Pn-CYP3027F2在响应WAF的过程中迅速显着上调，可作为WAF暴露浓度40％或更高浓度的有用生物标记。本文将有助于更好地了解co足动物中环境有毒物质代谢的分子机制事件。

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  • 来源
    《Environmental Science & Technology》 |2015年第11期|6982-6992|共11页
  • 作者

    Jeonghoon Han; Eun-Ji Won; Hui-Su Kim; David R. Nelson; Su-Jae Lee; Heum Gi Park; Jae-Seong Lee;

  • 作者单位

    Department of Biological Science, College of Science, Sungkyunkwan University, Suwon, Gyeonggi-do 440-746, South Korea;

    Department of Biological Science, College of Science, Sungkyunkwan University, Suwon, Gyeonggi-do 440-746, South Korea;

    Department of Biological Science, College of Science, Sungkyunkwan University, Suwon, Gyeonggi-do 440-746, South Korea;

    Department of Microbiology, Immunology, and Biochemistry, University of Tennessee, Memphis, Tennessee 38163, United States;

    Department of Life Sciences, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea;

    Department of Marine Resource Development, College of Life Sciences, Gangneung-Wonju National University, Gangneung, Gangwon-do 210-702, South Korea;

    Department of Biological Science, College of Science, Sungkyunkwan University, Suwon, Gyeonggi-do 440-746, South Korea;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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