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首页> 外文期刊>Environmental Science & Technology >Dynamics of Metal Partitioning at the Cell-Solution Interface: Implications for Toxicity Assessment under Growth-Inhibiting Conditions
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Dynamics of Metal Partitioning at the Cell-Solution Interface: Implications for Toxicity Assessment under Growth-Inhibiting Conditions

机译：细胞-溶液界面处金属分配的动力学：生长抑制条件下毒性评估的意义。

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摘要

Metal toxicity toward microorganisms is usually evaluated by determining growth inhibition. To achieve a mechanistic interpretation of such toxic effects, the intricate coupling between cell growth kinetics and metal partitioning dynamics at the cell-solution interface over time must be considered on a quantitative level. A formalism is elaborated to evaluate cell-surface-bound, internalized, and extracellular metal fractions in the limit where metal uptake kinetics is controlled by internalization under noncomplexing medium conditions. Cell growth kinetics is tackled using the continuous logistic equation modified to include growth inhibition by metal accumulation to intracellular or cell surface sites. The theory further includes metal-proton competition for adsorption at cell-surface binding sites, as well as possible variation of cell size during exposure to metal ions. The formalism eluddates the dramatic impacts of initial cell concentration on metal bioavailability and toxidty over time, in agreement with reported algae bioassays. It further highlights that appropriate definition of toxicity endpoints requires careful inspection of the ratio between exposure time scale and time scale of metal depletion from bulk solution. The latter depends on metal internalization-excretion rate constants, microorganism growth, and the extent of metal adsorption on nonspecific, transporter, and growth inhibitory sites. As an application of the theory, Cd toxicity in the algae Pseudokirchneriella subcapitata is interpreted from constrained modeling of cell growth kinetics and of interfacial Cd-partitioning dynamics measured under various exposure conditions.
机译：金属对微生物的毒性通常通过确定生长抑制来评估。为了对这种毒性作用进行机械解释，必须在定量水平上考虑细胞生长动力学与细胞-溶液界面处的金属分配动力学之间的复杂耦合。详细阐述了形式学，以评估在非复杂培养基条件下通过内化作用控制金属摄取动力学的极限内的细胞表面结合，内化和细胞外金属部分。使用连续逻辑方程对细胞生长动力学进行了处理，该方程被修改为包括通过金属积累到细胞内或细胞表面部位而抑制生长。该理论还包括在细胞表面结合位点吸附的金属质子竞争以及暴露于金属离子过程中细胞大小的可能变化。与报道的藻类生物测定法一致，形式主义消除了初始细胞浓度随时间的推移对金属生物利用度和氧化作用的巨大影响。它进一步强调，毒性终点的适当定义需要仔细检查暴露时间标度与散装溶液中金属消耗时间标度之间的比率。后者取决于金属内在化-分泌速率常数，微生物生长以及金属在非特异性，转运蛋白和生长抑制位点上的吸附程度。作为该理论的应用，可通过在各种暴露条件下测量的细胞生长动力学和界面Cd分配动力学的约束模型来解释藻类假单胞菌中Cd的毒性。

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  • 来源
    《Environmental Science & Technology》 |2015年第11期|6625-6636|共12页
  • 作者

    Jerome F. L. Duval; Nathalie Paquet; Michel Lavoie; Claude Fortin;

  • 作者单位

    Uboratoire Interdisdplinaire des Environnements Continentaux (LIEC), CNRS, UMR7360, Vandoeuvre-les-Nancy, F-54501, France ,Laboratoire Interdisdplinaire des Environnements Continentaux (LIEC), Universite de Lorraine, UMR7360, Vandoeuvre-les-Nancy, F-54501, France;

    Centre Eau Terre Environnement (INRS-ETE), Institut National de la Recherche Sdentifique, 490 de la Couronne, Quebec G1K 9A9, Canada,Centre d'expertise en analyse environnementale du Quebec, Ministere du Developpement durable, de l'Environnement et de la Lutte contre les changements climatiques (MDDELCC), 2700 Rue Einstein, G1P 3W8, Quebec, Canada;

    Centre Eau Terre Environnement (INRS-ETE), Institut National de la Recherche Sdentifique, 490 de la Couronne, Quebec G1K 9A9, Canada,Quebec-Ocean and Takuvik Joint Universite Laval/CNRS Research Units, Departement de Biologie, Universite Laval, G1V OA6, Quebec, Canada;

    Centre Eau Terre Environnement (INRS-ETE), Institut National de la Recherche Sdentifique, 490 de la Couronne, Quebec G1K 9A9, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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