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首页> 外文期刊>Environmental Science & Technology >Evaluation of Molecular- and Culture-Dependent MST Markers To Detect Fecal Contamination and Indicate Viral Presence in Good Quality Groundwater
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Evaluation of Molecular- and Culture-Dependent MST Markers To Detect Fecal Contamination and Indicate Viral Presence in Good Quality Groundwater

机译：评估依赖分子和文化的MST标记物以检测粪便污染并表明优质地下水中存在病毒

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摘要

Microbial contamination of groundwater represents a significant health risk to resource users. Culture-dependent Bacteroides phage and molecular-dependent Bacteroidales 16S rRNA assays are employed in microbial source tracking (MST) studies globally, however little is known regarding how these important groups relate to each other in the environment and which is more suitable to indicate the presence of waterborne fecal pollution and human enteric viruses. This study addresses this knowledge gap by examining 64 groundwater samples from sites with varying hydrogeological properties using a MST toolbox containing two bacteriophage groups (phage infecting GB-124 and ARABA-84), and two Bacteroidales 16S rRNA markers (Hf183 and BacR); those were compared to fecal indicator bacteria, somatic coliphage, Bacteroidales 16S rRNA marker AllBac, four human enteric viruses (norovirus GⅠ and Ⅱ, enterovirus and group A rotavirus) and supplementary hydrogeological/chemical data. Bacteroidales 16S rRNA indicators offered a more sensitive assessment of both human-specific and general fecal contamination than phage indicators, but may overestimate the risk from enteric viral pathogens. Comparison with hydrogeological and land use site characteristics as well as auxiliary microbiological and chemical data proved the plausibility of the MST findings. Sites representing karst aquifers were of significantly worse microbial quality than those with unconsolidated or fissured aquifers, highlighting the vulnerability of these hydrogeological settings.
机译：地下水的微生物污染对资源使用者构成重大健康风险。微生物来源跟踪（MST）研究在全球范围内采用了依赖于培养物的拟杆菌噬菌体和依赖分子的拟杆菌属16S rRNA分析法，然而，关于这些重要基团在环境中如何相互关联的知之甚少，它更适合于表明存在粪便中的水污染和人类肠道病毒。这项研究通过使用包含两个噬菌体组（感染GB-124和ARABA-84的噬菌体）和两个拟杆菌16S rRNA标记（Hf183和BacR）的MST工具箱检查了具有不同水文地质性质的地点的64个地下水样本，从而解决了这一知识鸿沟。将它们与粪便指示细菌，体细胞大肠杆菌噬菌体，细菌科16S rRNA标记AllBac，四种人类肠道病毒（诺如病毒GⅠ和Ⅱ，肠道病毒和A组轮状病毒）以及补充的水文地质/化学数据进行了比较。与噬菌体指标相比，细菌科16S rRNA指标对人特异性和一般粪便污染的评估更为敏感，但可能会高估肠道病毒病原体的风险。与水文地质和土地利用场所特征以及辅助的微生物和化学数据进行比较，证明了MST研究结果的合理性。代表岩溶含水层的地点的微生物质量比没有固结或裂隙含水层的地点的微生物质量明显差，这突出了这些水文地质环境的脆弱性。

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  • 来源
    《Environmental Science & Technology》 |2015年第12期|7142-7151|共10页
  • 作者

    D. Diston; M. Sinreich; S. Zimmermann; A. Baumgartner; R. Felleisen;

  • 作者单位

    Federal Food Safety and Veterinary Office FSVO, Bern 3003, Switzerland;

    Federal Office for the Environment FOEN, Bern 3003, Switzerland;

    Federal Office for the Environment FOEN, Bern 3003, Switzerland;

    Federal Food Safety and Veterinary Office FSVO, Bern 3003, Switzerland;

    Federal Food Safety and Veterinary Office FSVO, Bern 3003, Switzerland;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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