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首页> 外文期刊>Environmental Science & Technology >Real-Time Visualization of Perylene Nanoclusters in Water and Their Partitioning to Graphene Surface and Macrophage Cells
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Real-Time Visualization of Perylene Nanoclusters in Water and Their Partitioning to Graphene Surface and Macrophage Cells

机译：水中of纳米簇的实时可视化及其在石墨烯表面和巨噬细胞中的分配

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摘要

Hydrophobic organic chemicals (HOCs) are of special ecotoxicological concern because they can be directly incorporated and bioconcentrated in living organisms. However, the effects of self-clustering of HOCs on their environmental behavior and toxicology have not yet received enough attention. With the use of a recently developed technique, single-molecule fluorescence microscopy, the motion and distribution of perylene nanoclusters (PNCs) formed in water at very low concentration (1 μM) were visualized with high temporal and spatial resolution. The liquid-solid interface process of PNCs adsorbing onto graphene was also recorded. Instead of the traditional view of HOC adsorption as a single molecule, our study revealed the characteristic of irreversible adsorption of perylene onto the carbonaceous surface in the form of nanoclusters, exhibiting random sequential "car-parking" events. More interestingly, the transport of PNCs across the cell membrane was also captured in real time, demonstrating that they entered macrophage cells by endocytosis. Supplementing the well-recognized routine of passive diffusion through a membrane lipid bilayer, the uptake of HOCs in the form of nanoclusters by endocytosis is proposed to be an additional but important mechanism for their uptake into living cells. The distribution of HOCs in environmental systems in the form of nanoclusters, exemplified by PNCs in this study, may have significant implications for understanding their environmental fate and potential toxicological effects.
机译：疏水性有机化学品（HOC）具有特别的生态毒理学意义，因为它们可以直接掺入生物体内并进行生物浓缩。但是，HOC的自聚类对其环境行为和毒理学的影响尚未引起足够的重视。通过使用最新开发的技术（单分子荧光显微镜），可以以高时间和空间分辨率可视化在水中以极低的浓度（1μM）形成的per纳米簇（PNC）的运动和分布。还记录了PNC吸附在石墨烯上的液-固界面过程。不同于传统的HOC吸附是单分子的观点，我们的研究揭示了per以纳米团簇的形式不可逆地吸附到碳质表面上的特征，表现出随机顺序的“停车”事件。更有趣的是，PNC跨细胞膜的转运也被实时捕获，表明它们是通过内吞作用进入巨噬细胞的。作为公认的通过膜脂质双层被动扩散的常规方法的补充，通过内吞作用摄取呈纳米团簇形式的HOC被认为是其被摄取到活细胞中的另一重要机制。本研究中以PNCs为例，HOCs以纳米团簇的形式在环境系统中的分布可能对理解其环境命运和潜在的毒理学效应具有重要意义。

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  • 来源
    《Environmental Science & Technology》 |2015年第13期|7926-7933|共8页
  • 作者

    Xuejun Guo; Xin Jin; Xiaofang Lv; Yingying Pu; Fan Bai;

  • 作者单位

    State Key Laboratory of Environment Simulation, School of Environment, Beijing Normal University, No. 19 Xinjiekouwai Street, Beijing 100875, China;

    State Key Laboratory of Environment Simulation, School of Environment, Beijing Normal University, No. 19 Xinjiekouwai Street, Beijing 100875, China;

    State Key Laboratory of Environment Simulation, School of Environment, Beijing Normal University, No. 19 Xinjiekouwai Street, Beijing 100875, China;

    Biodynamic Optical Imaging Center, School of Life Science, Peking University, No. 5 Yiheyuan Road, Beijing 100871, China;

    Biodynamic Optical Imaging Center, School of Life Science, Peking University, No. 5 Yiheyuan Road, Beijing 100871, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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