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首页> 外文期刊>Environmental Science & Technology >Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris
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Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris

机译：红假单胞菌在玉米秸秆水解产物中代谢多种芳香族化合物

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摘要

Lignocellulosic biomass hydrolysates hold great potential as a feedstock for microbial biofuel production, due to their high concentration of fermentable sugars. Present at lower concentrations are a suite of aromatic compounds that can inhibit fermentation by biofuel-producing microbes. We have developed a microbial-mediated strategy for removing these aromatic compounds, using the purple nonsulfur bacterium Rhodopseudomonas palustris. When grown photo- heterotrophically in an anaerobic environment, R. palustris removes most of the aromatics from ammonia fiber expansion (AFEX) treated corn stover hydrolysate (ACSH), while leaving the sugars mostly intact. We show that R. palustris can metabolize a host of aromatic substrates in ACSH that have either been previously described as unable to support growth, such as methoxylated aromatics, and those that have not yet been tested, such as aromatic amides. Removing the aromatics from ACSH with R. palustris, allowed growth of a second microbe that could not grow in the untreated ACSH. By using defined mutants, we show that most of these aromatic compounds are metabolized by the benzoyl-CoA pathway. We also show that loss of enzymes in the benzoyl-CoA pathway prevents total degradation of the aromatics in the hydrolysate, and instead allows for biological transformation of this suite of aromatics into selected aromatic compounds potentially recoverable as an additional bioproduct.
机译：木质纤维素生物质水解产物由于其高浓度的可发酵糖具有巨大的潜力，可作为微生物生物燃料生产的原料。较低浓度的芳香族化合物可以抑制产生生物燃料的微生物发酵。我们已经开发了一种微生物介导的策略，可以使用紫色无硫细菌紫红假单胞菌（Rhodopseudomonas palustris）去除这些芳香族化合物。当在厌氧环境中光异养生长时，R。palustris会从氨纤维膨胀（AFEX）处理过的玉米秸秆水解产物（ACSH）中去除大部分芳香族化合物，同时使糖类大部分保持完整。我们表明，R。palustris可以代谢ACSH中的许多芳香族底物，这些芳香底物先前已被描述为无法支持生长，例如甲氧基化的芳香族化合物，而尚未经过测试的那些，例如芳香族酰胺。用palustris去除ACSH中的芳香剂，使第二种微生物无法在未经处理的ACSH中生长。通过使用定义的突变体，我们表明，这些芳香族化合物中的大多数通过苯甲酰基-辅酶A途径代谢。我们还表明，苯甲酰辅酶A途径中酶的丢失阻止了水解产物中芳族化合物的完全降解，而是允许将这组芳族化合物生物转化为可能作为另外的生物产品回收的所选芳族化合物。

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  • 来源
    《Environmental Science & Technology》 |2015年第14期|8914-8922|共9页
  • 作者

    Samantha Austin; Wayne S. Kontur; Arne Ulbrich; J. Zachary Oshlag; Weiping Zhang; Alan Higbee; Yaoping Zhang; Joshua J. Coon; David B. Hodge; Timothy J. Donohue; Daniel R. Noguera;

  • 作者单位

    Department of Civil and Environmental Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States,DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States;

    Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States,DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States;

    Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States;

    Department of Civil and Environmental Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States,DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States;

    Department of Civil and Environmental Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States,DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States;

    DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States,Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States;

    DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States;

    DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States,Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States,Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States;

    Department of Chemical Engineering & Materials Science, Michigan State University, East Lansing, Michigan 48824, United States,Department of Biosystems & Agricultural Engineering, Michigan State University, East Lansing, Michigan 48824, United States;

    Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States,DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States;

    Department of Civil and Environmental Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706,Department of Civil and Environmental Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States,DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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