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首页> 外文期刊>Environmental Science & Technology >Stable Carbon Isotope Fractionation During 1,2-Dichloropropane-to-Propene Transformation by an Enrichment Culture Containing Dehalogenimonas Strains and a dcpA Gene
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Stable Carbon Isotope Fractionation During 1,2-Dichloropropane-to-Propene Transformation by an Enrichment Culture Containing Dehalogenimonas Strains and a dcpA Gene

机译：包含Dehalogenimonas菌株和dcpA基因的富集培养物在1,2-二氯丙烷向丙烯转化过程中的稳定碳同位素分馏

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摘要

A stable enrichment culture derived from Besos river estuary sediments stoichiometrically dechlorinated 1,2-dichloropropane (1,2-DCP) to propene. Sequential transfers in defined anaerobic medium with the inhibitor bromoethanesulfonate produced a sediment-free culture dechlorinating 1,2-DCP in the absence of methanogenesis. Application of previously published genus-specific primers targeting 16S rRNA gene sequences revealed the presence of a Dehalogenimonas strain, and no amplification was obtained with Dehalococcoides-specific primers. The partial sequence of the 16S rRNA amplicon was 100% identical with Dehalogenimonas alkenigignens strain IP3-3. Also, dcpA, a gene described to encode a corrinoid-containing 1,2-DCP reductive dehalogenase was detected. Resistance of the dehalogenating activity to vancomycin, exclusive conversion of vicinally chlorinated alkanes, and tolerance to short-term oxygen exposure is consistent with the hypothesis that a Dehalogenimonas strain is responsible for 1,2-DCP conversion in the culture. Quantitative PCR showed a positive correlation between the number of Dehalogenimonas 16S rRNA genes copies in the culture and consumption of 1,2-DCP. Compound specific isotope analysis revealed that the Dehalogenimonas-catslyzed carbon isotopic fractionation (εO_(bulk)) of the 1,2-DCP-to-propene reaction was -15.0 ± 0.7‰ under both methanogenic and nonmethanogenic conditions. This study demonstrates that carbon isotope fractionation is a valuable approach for monitoring in situ 1,2-DCP reductive dechlorination by Dehalogenimonas strains.
机译：一种稳定的富集培养物，来自贝索斯河河口沉积物，化学计量地将1,2-二氯丙烷（1,2-DCP）脱氯成丙烯。在限定的厌氧培养基中用溴乙烷磺酸盐抑制剂进行的连续转移产生了无沉淀的培养物，在没有产甲烷作用的情况下将1,2-DCP脱氯。先前发布的针对16S rRNA基因序列的属特异性引物的应用揭示了Dehalogenimonas菌株的存在，并且用Dehalococcoides特异性引物未获得扩增。 16S rRNA扩增子的部分序列与Dehalogenimonas alkenigignens菌株IP3-3 100％相同。此外，还检测到了dcpA，一种被描述为编码含有类视黄醇的1,2-DCP还原脱卤素酶的基因。脱卤活性对万古霉素的抗性，毒氯代烷烃的排他性转化以及对短期氧气暴露的耐受性与以下假设相符：脱卤单胞菌菌株是培养物中1,2-DCP转化的原因。定量PCR显示培养物中Dehalogenimonas 16S rRNA基因拷贝数与1,2-DCP消耗之间呈正相关。化合物的特异性同位素分析表明，在产甲烷和非甲烷生成条件下，1,2-DCP-丙烯反应的脱卤代单核素催化的碳同位素分馏（εO_（bulk））为-15.0±0.7‰。这项研究表明，碳同位素分级分离是监测Dehalogenimonas菌株原位1,2-DCP还原脱氯的一种有价值的方法。

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  • 来源
    《Environmental Science & Technology》 |2015年第14期|8666-8674|共9页
  • 作者

    L. Martin-Gonzalez; S. Hatijah Mortan; M. Rosell; E. Parlade; M. Martinez-Alonso; N. Gaju; G. Caminal; L. Adrian; E. Marco-Urrea;

  • 作者单位

    Departament d'Enginyeria Quimica, Universitat Autonoma de Barcelona (UAB), Carrer de les Sitges s, 08193 Bellaterra, Spain;

    Departament d'Enginyeria Quimica, Universitat Autonoma de Barcelona (UAB), Carrer de les Sitges s, 08193 Bellaterra, Spain;

    Departament de Crystal-lografia, Mineralogia I Diposits Minerals, Facultat de Geologia, Universitat de Barcelona (UB), Marti Franques s, 08028. Barcelona, Spain;

    Departament de Genetica I Microbiologia, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain;

    Departament de Genetica I Microbiologia, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain;

    Departament de Genetica I Microbiologia, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain;

    Institut de Quimica Avancada de Catalunya (IQAC) CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain;

    Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, Leipzig, Germany;

    Departament d'Enginyeria Quimica, Universitat Autonoma de Barcelona (UAB), Carrer de les Sitges s, 08193 Bellaterra, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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