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首页> 外文期刊>Environmental Science & Technology >Naphthenic Acid Mixtures from Oil Sands Process-Affected Water Enhance Differentiation of Mouse Embryonic Stem Cells and Affect Development of the Heart
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Naphthenic Acid Mixtures from Oil Sands Process-Affected Water Enhance Differentiation of Mouse Embryonic Stem Cells and Affect Development of the Heart

机译：受油砂处理影响的水中的环烷酸混合物增强了小鼠胚胎干细胞的分化并影响了心脏的发育

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摘要

Extraction of petrochemicals from the surface mining of oil sand deposits results in generation of large volumes of oil sands process-affected water (OSPW). Naphthenic acids (NA) are generally considered to be among the most toxic components of OSPW. Previous studies have shown that NAs are toxic to aquatic organisms, however knowledge of their effects on mammalian health and development is limited. In the present study, we evaluated the developmental effects of an NA extract prepared from fresh OSPW on differentiating mouse embryonic stem cells (ESC). We found that treatment of differentiating cells with the NA extract at noncytotoxic concentrations alters expression of various lineage specification markers and development of the heart. Notably, expression of cardiac specific markers such as Nkx2.5, Gata4, and Mef2c were significantly up-regulated. Moreover, exposure to the NA extract enhanced differentiation of embryoid bodies and resulted in the early appearance of spontaneously beating clusters. Interestingly, exposure of undifferentlated mouse ESCs to the NA extract did not change the expression level of pluripotency markers (i.e., Oct4, Nanog, and Sox2). Altogether, these data identify some of the molecular pathways affected by components within this NA extract during differentiation of mammalian cells.
机译：从油砂沉积物的地面采矿中提取石油化学物质会产生大量受工艺影响的油砂（OSPW）。环烷酸（NA）通常被认为是OSPW毒性最大的成分之一。先前的研究表明，NAs对水生生物有毒，但是对它们对哺乳动物健康和发育的影响的了解有限。在本研究中，我们评估了由新鲜OSPW制备的NA提取物对分化小鼠胚胎干细胞（ESC）的发育作用。我们发现，用非细胞毒性浓度的NA提取物处理分化细胞会改变各种谱系规格标记的表达和心脏的发育。值得注意的是，心脏特异性标志物（如Nkx2.5，Gata4和Mef2c）的表达显着上调。此外，暴露于NA提取物可增强胚状体的分化，并导致自发跳动簇的早期出现。有趣的是，未分化的小鼠胚胎干细胞暴露于NA提取物不会改变多能性标志物（即Oct4，Nanog和Sox2）的表达水平。总而言之，这些数据确定了在哺乳动物细胞分化期间受该NA提取物中的组分影响的一些分子途径。

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  • 来源
    《Environmental Science & Technology》 |2015年第16期|10165-10172|共8页
  • 作者

    Paria Mohseni; Noah A. Hahn; Richard A. Frank; L. Mark Hewitt; Mehrdad Hajibabaei; Glen Van Der Kraak;

  • 作者单位

    Department of Integrative Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada;

    Department of Integrative Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada ,Department of Molecular Genetics, University of Toronto, Toronto, Ontario, M5S 1A8 Canada;

    Water Science and Technology Directorate, Environment Canada, Burlington, Ontario L7S 1A1, Canada;

    Water Science and Technology Directorate, Environment Canada, Burlington, Ontario L7S 1A1, Canada;

    Department of Integrative Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada ,Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario N1G 2W1, Canada;

    Department of Integrative Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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