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首页> 外文期刊>Environmental Science & Technology >Degradation of Amino Acids and Structure in Model Proteins and Bacteriophage MS2 by Chlorine, Bromine, and Ozone
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Degradation of Amino Acids and Structure in Model Proteins and Bacteriophage MS2 by Chlorine, Bromine, and Ozone

机译：氯，溴和臭氧降解模型蛋白和噬菌体MS2中的氨基酸和结构

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摘要

Proteins are important targets of chemical disinfectants. To, improve the understanding of disinfectant-protein reactions, this study characterized the disinfectant:protein molar ratios at which 50% degradation of oxidizable amino adds (i.e., Met, Tyr, Trp, His, Lys) and structure were observed during HOCl, HOBr, and O_3 treatment of three well-characterized model proteins and bacteriophage MS2. A critical question is the extent to which the targeting of amino adds is driven by their disinfectant rate constants rather man their geometrical arrangement. Across the model proteins and bacteriophage MS2 (coat protein), differing widely in structure, methionine was preferentially targeted, forming predominantly metbionine sulfoxide. This targeting concurs with its high disinfectant rate constants and supports its hypothesized role as a sacrificial antioxldant. Despite higher HOCl and HOBr rate constants with histidine and lysine than for tyrosine, tyrosine generally was degraded in preference to histidine, and to a lesser extent, lysine. These results concur with the prevalence of geometrical motifs featuring histidines or lysines near tyrosines, facilitating histidine and lysine regeneration upon Cl[+1] transfer from their chloramines to tyrosines. Lysine nitrile formation occurred at or above oxidant doses where 3,5-dihalotyrosine products began to degrade. For O_3, which lacks a similar oxidant transfer pathway, histidine, tyrosine, and lysine degradation followed their relative O_3 rate constants. Except for its low reactivity with lysine, the O_3 doses required to degrade amino adds were as low as or lower man for HOCl or HOBr, indicating its cxidative efficiency. Loss of structure did not correlate with loss of particular amino adds, suggesting the need to characterize the oxidation of specific geometric motifs to understand structural degradation.
机译：蛋白质是化学消毒剂的重要目标。为了提高对消毒剂-蛋白质反应的理解，本研究对消毒剂：蛋白质的摩尔比进行了表征，在HOCl，HOBr期间观察到50％可氧化氨基的降解（即Met，Tyr，Trp，His，Lys）和结构降解。 ，以及O_3处理三种特征明确的模型蛋白和噬菌体MS2。一个关键的问题是，氨基添加的靶向程度是由其消毒剂速度常数而不是其几何排列决定的。在模型蛋白质和噬菌体MS2（外壳蛋白）之间，结构差异很大，蛋氨酸被优先靶向，主要形成甲硫氨酸亚砜。这种靶向具有较高的消毒速率常数，并支持其作为牺牲性抗氧化剂的假设作用。尽管与酪氨酸相比，组氨酸和赖氨酸的HOCl和HOBr速率常数更高，但酪氨酸通常优先于组氨酸降解，而赖氨酸降解程度较小。这些结果与以酪氨酸附近的组氨酸或赖氨酸为特征的几何基序普遍存在，当Cl [+1]从其氯胺转移至酪氨酸时，促进了组氨酸和赖氨酸的再生。在3,5-二卤代酪氨酸产物开始降解的氧化剂剂量或以上时，会形成赖氨酸腈。对于缺少类似氧化剂转移途径的O_3，组氨酸，酪氨酸和赖氨酸的降解遵循其相对的O_3速率常数。除了其与赖氨酸的低反应性外，降解氨基添加所需的O_3剂量与HOCl或HOBr一样低或更低，表明其氧化效率很高。结构损失与特定氨基酸添加的损失不相关，表明需要表征特定几何基序的氧化以了解结构降解。

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  • 来源
    《Environmental Science & Technology》 |2015年第22期|13331-13339|共9页
  • 作者

    Jong Kwon Choe; David H. Richards; Corey J. Wilson; William A. Mitch;

  • 作者单位

    Department of Civil and Environmental Engineering, Stanford University, Stanford, California 94305, United States;

    Department of Chemical and Environmental Engineering, Yale University, New Haven, Connecticut 06520, United States;

    Department of Chemical and Environmental Engineering, Yale University, New Haven, Connecticut 06520, United States;

    Department of Civil and Environmental Engineering, Stanford University, Stanford, California 94305, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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