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Impact of Heavy Metals on Transcriptional and Physiological Activity of Nitrifying Bacteria

机译：重金属对硝化细菌转录和生理活性的影响

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摘要

Heavy metals can inhibit nitrification, a key process for nitrogen removal in wastewater treatment. The transcriptional responses of amoA, hao, nirK, and norB were measured in conjunction with specific oxygen uptake rate (sOUR) for nitrifying enrichment cultures exposed to different metals (Ni(Ⅱ), Zn(Ⅱ), Cd(Ⅱ), and Pb(Ⅱ)). There was significant decrease in sOUR with increasing concentrations for Ni(Ⅱ) (0.03-3 mg/L), Zn(Ⅱ) (0.1-10 mg/L), and Cd(Ⅱ) (0.03-1 mg/L) (p ＜ 005). However, no considerable changes in sOUR wen observed with Pb(Ⅱ) (1-100 mg/L), except at a dosage of 1000 mg/L causing 8496 inhibition. Based on RT- qPCR data, the transcript levels of amoA and hao decreased when exposed to Ni(Ⅱ) dosages. Slight up-regulation of amoA, hao, and nirK(05-13-fold) occurred after exposure to 0.3-3 mg/L Zn(Ⅱ), although their expression decreased for 10 mg/L Zn(Ⅱ). With the exception of 1000 mg/L Pb(Ⅱ), stimulation of all genes occurred on Cd(Ⅱ) and Pb(Ⅱ) exposure. While overall the results show that RNA-based function-spedfic assays can be used as potential surrogates for measuring nitrification activity, the degree of inhibition inferred from sOUR and gene transcription is different We suggest that variations in transcription of functional genes may supplement sOUR based assays as early warning indicators of upsets in nitrification.
机译：重金属可以抑制硝化作用，这是废水处理中脱氮的关键过程。在暴露于不同金属（Ni（Ⅱ），Zn（Ⅱ），Cd（Ⅱ）和Pb）的硝化富集培养物中，结合特定的氧气吸收率（sOUR），测量了amoA，hao，nirK和norB的转录反应。 （Ⅱ））。 Ni（Ⅱ）（0.03-3 mg / L），Zn（Ⅱ）（0.1-10 mg / L）和Cd（Ⅱ）（0.03-1 mg / L）的浓度随sOUR的增加而显着降低（ p ＜005）。然而，Pb（Ⅱ）（1-100 mg / L）观察到的sOUR wen没有明显变化，除了1000 mg / L的剂量引起8496抑制作用。根据RT-qPCR数据，暴露于Ni（Ⅱ）剂量时，amoA和hao的转录水平降低。暴露于0.3-3 mg / L Zn（Ⅱ）后，amoA，hao和nirK发生轻微上调（05-13倍），但10 mg / L Zn（Ⅱ）的表达下降。除1000 mg / L Pb（Ⅱ）外，所有基因均在Cd（Ⅱ）和Pb（Ⅱ）暴露下发生刺激。虽然总体结果表明基于RNA的功能饱和检测可以用作测量硝化活性的潜在替代物，但从sOUR和基因转录推断出的抑制程度是不同的。我们建议功能基因转录的变异可能会补充基于sOUR的检测作为硝化作用不正常的预警指标。

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  • 来源
    《Environmental Science & Technology》 |2015年第22期|13454-13462|共9页
  • 作者

    Vikram Kapoor; Xuan Li; Michael Elk; Kartik Chandran; Christopher A. Impellitteri; Jorge W. Santo Domingo;

  • 作者单位

    U.S. Environmental Protection Agency, Office of Research and Development, Cincinnati, Ohio 45268, United States;

    U.S. Environmental Protection Agency, Office of Research and Development, Cincinnati, Ohio 45268, United States;

    Pegasus Technical Services, Inc., Cincinnati, Ohio 45268, United States;

    Department of Earth and Environmental Engineering, Columbia University, New York, New York 10027, United States;

    U.S. Environmental Protection Agency, Office of Research and Development, Cincinnati, Ohio 45268, United States;

    U.S. Environmental Protection Agency, Office of Research and Development, Cincinnati, Ohio 45268, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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