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首页> 外文期刊>Environmental Science & Technology >Combined Flux Chamber and Genomics Approach Links Nitrous Acid Emissions to Ammonia Oxidizing Bacteria and Archaea in Urban and Agricultural Soil
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Combined Flux Chamber and Genomics Approach Links Nitrous Acid Emissions to Ammonia Oxidizing Bacteria and Archaea in Urban and Agricultural Soil

机译:通量室和基因组学相结合的方法将亚硝酸排放与城市和农业土壤中的氨氧化细菌和古生菌联系起来

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摘要

Nitrous acid (HONO) is a photochemical source of hydroxyl radical and nitric oxide in the atmosphere that stems from abiotic and biogenic processes, including the activity of ammonia-oxidizing soil microbes. HONO fluxes were measured from agricultural and urban soil in mesocosm studies aimed at characterizing biogenic sources and linking them to indigenous microbial consortia. Fluxes of HONO from agricultural and urban soil were suppressed by addition of a nitrification inhibitor and enhanced by amendment with ammonium (NH_4~+), with peaks at 19 and 8 ng m~(-2) s~(-1), respectively. In addition, both agricultural and urban soils were observed to convert ~(15)NH_4~+ to HO~(15)NO. Genomic surveys of soil samples revealed that 1.5-6% of total expressed 16S rRNA sequences detected belonged to known ammonia oxidizing bacteria and archaea. Peak fluxes of HONO were directly related to the abundance of ammonia-oxidizer sequences, which in turn depended on soil pH. Peak HONO fluxes under fertilized conditions are comparable in magnitude to fluxes reported during field campaigns. The results suggest that biogenic HONO emissions will be important in soil environments that exhibit high nitrification rates (e.g., agricultural soil) although the widespread occurrence of ammonia oxidizers implies that biogenic HONO emissions are also possible in the urban and remote environment.
机译:亚硝酸(HONO)是大气中的羟基自由基和一氧化氮的光化学源,其源于非生物和生物过程,包括氨氧化土壤微生物的活性。在中尺度研究中,从农业和城市土壤中测量了HONO通量,旨在表征生物源并将其与本地微生物群落联系起来。加入硝化抑制剂可抑制农业和城市土壤中HONO的通量,并通过铵盐(NH_4〜+)的添加而增强,其峰值分别在19和8 ng m〜(-2)s〜(-1)。此外,观察到农业和城市土壤均将〜(15)NH_4〜+转化为HO〜(15)NO。对土壤样品的基因组调查显示,检测到的总表达16S rRNA序列的1.5-6%属于已知的氨氧化细菌和古细菌。 HONO的峰值通量与氨-氧化剂序列的丰度直接相关,而氨-氧化剂的丰度又取决于土壤的pH值。受精条件下的峰值HONO通量在强度上与野战期间报告的通量相当。结果表明,尽管氨氧化剂的广泛存在意味着在城市和边远环境中也可能发生生物HONO排放,但在具有高硝化率的土壤环境(例如农业土壤)中,生物HONO的排放将很重要。

著录项

  • 来源
    《Environmental Science & Technology》 |2015年第23期|13825-13834|共10页
  • 作者单位

    School of Public and Environmental Affairs, Indiana University, Bloomington, Indiana 47405-2204, United States;

    Integrated Program in the Environment, Indiana University, Bloomington, Indiana 47405-2204, United States;

    School of Public and Environmental Affairs, Indiana University, Bloomington, Indiana 47405-2204, United States,Department of Chemistry, Smith College, Northampton, Massachusetts 01063;

    School of Public and Environmental Affairs, Indiana University, Bloomington, Indiana 47405-2204, United States;

    School of Public and Environmental Affairs, Indiana University, Bloomington, Indiana 47405-2204, United States;

    School of Public and Environmental Affairs, Indiana University, Bloomington, Indiana 47405-2204, United States;

    Center for Genomics and Bioinformatics, Indiana University, Bloomington, Indiana 47405-7005, United States;

    School of Public and Environmental Affairs, Indiana University, Bloomington, Indiana 47405-2204, United States,Integrated Program in the Environment, Indiana University, Bloomington, Indiana 47405-2204, United States;

    School of Public and Environmental Affairs, Indiana University, Bloomington, Indiana 47405-2204, United States,Department of Chemistry, Indiana University, Bloomington, Indiana 47405-7102, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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