...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >Long-Term Incubation Reveals Methanogenic Biodegradation of C_5 and C_6 iso-Alkanes in Oil Sands Tailings
【24h】

Long-Term Incubation Reveals Methanogenic Biodegradation of C_5 and C_6 iso-Alkanes in Oil Sands Tailings

机译：长期温育揭示了油砂尾矿中C_5和C_6异烷烃的产甲烷生物降解作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Iso-Alkanes are major components of petroleum and have been considered recalcitrant to biodegradation under methanogenic conditions. However, indigenous microbes in oil sands tailings ponds exposed to solvents rich in 2-methylbutane, 2- methylpentane, 3-methylpentane, n-pentane, and n-hexane produce methane in situ. We incubated defined mixtures of iso- or n-alkanes with mature fine tailings from two tailings ponds of different ages historically exposed to different solvents: one, ～10 years old, receiving C_5-C_6 paraffins and the other, ～35 years old, receiving naphtha. A lengthy incubation (＞6 years) revealed iso-alkane biodegradation after lag phases of 900-1800 and ～280 days, respectively, before the onset of methanogenesis, although lag phases were shorter with M-alkanes (～650-1675 and ～170 days, respectively). 2-Methylpentane and both n-alkanes were completely depleted during ～2400 days of incubation, whereas 2- methylbutane and 3-methylpentane were partially depleted only during active degradation of 2-methylpentane, suggesting co-metabolism. In both cases, pyrotag sequencing of 16S rRNA genes showed codominance of Peptococcaceae with acetoclastic (Methanosaeta) and hydrogenotrophic (Methanoregula and Methanolinea) methanogens. These observations are important for predicting long-term greenhouse-gas emissions from oil sands tailings ponds and extend the known range of hydrocarbons susceptible to methanogenic biodegradation in petroleum-impacted anaerobic environments.
机译：异烷烃是石油的主要成分，被认为在产甲烷条件下难以降解。但是，油砂尾矿池中的原生微生物暴露于富含2-甲基丁烷，2-甲基戊烷，3-甲基戊烷，正戊烷和正己烷的溶剂中，会原位产生甲烷。我们将定义的异链烷烃或正构烷烃与成熟细尾矿的混合物从两个不同年龄的尾矿池中进行了培养，这些尾矿池历来暴露于不同的溶剂中：一个〜10岁，接受C_5-C_6石蜡，另一个〜35岁，接受石脑油。长时间的孵化（＞ 6年）显示，在甲烷生成开始之前，滞后阶段分别在900-1800和〜280天后异链烷烃生物降解，尽管M-烷烃的滞后阶段较短（〜650-1675和〜170）天）。在约2400天的培养过程中，2-甲基戊烷和两种正构烷烃都被完全消耗掉了，而2-甲基丁烷和3-甲基戊烷仅在2-甲基戊烷的活性降解过程中被部分消耗掉了，这表明存在共代谢。在这两种情况下，对16S rRNA基因的热标记测序都表明，肽球菌科与乙酰碎裂菌（Methanosaeta）和氢营养菌（Methanoregula和Methanolinea）产甲烷菌具有共性。这些观察对于预测油砂尾矿池的长期温室气体排放非常重要，并扩展了在石油影响的厌氧环境中易产甲烷生物降解的碳氢化合物的已知范围。

著录项

  • 来源
    《Environmental Science & Technology》 |2015年第24期|14732-14739|共8页
  • 作者

    Tariq Siddique; Mohd Faidz Mohamad Shahimin; Saima Zamir; Kathleen Semple; Carmen Li; Julia M. Foght;

  • 作者单位

    Department of Renewable Resources, University of Alberta, Edmonton, AB T6G 2G7, Canada;

    Department of Renewable Resources, University of Alberta, Edmonton, AB T6G 2G7, Canada;

    Department of Renewable Resources, University of Alberta, Edmonton, AB T6G 2G7, Canada;

    Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada;

    Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada;

    Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号