Intra- and Intercellular Silver Nanoparticle Translocation and Transformation in Oyster Gill Filaments: Coupling Nanoscale Secondary Ion Mass Spectrometry and Dual Stable Isotope Tracing Study

Zishuang Shao; Paul Guagliardo; Haibo Jiang; Wen-Xiong Wang

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Intra- and Intercellular Silver Nanoparticle Translocation and Transformation in Oyster Gill Filaments: Coupling Nanoscale Secondary Ion Mass Spectrometry and Dual Stable Isotope Tracing Study

机译：牡蛎鳃细胞内和细胞间银纳米粒子易位和转化：偶极纳米级二次离子质谱和双稳态同位素跟踪研究

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The extensive application of silver nanoparticles (AgNPs) requires a full examination of their biological impacts, especially in aquatic systems where AgNPs are likely to end up. Despite numerous toxicity studies from molecular to individual levels, it is still a daunting challenge to achieve in situ subcellular imaging of Ag and to determine the sites of AgNP interaction with organelles or macromolecules simultaneously.Here, by coupling high-resolution nanoscale secondary ion mass spectrometry elemental mapping with scanning electron microscopy ultra structural characterization, we successfully visualized the subcellular localization and the potential toxicity effects of AgNPs in the oyster gill filaments. The stable isotope tracing method was also adopted to investigate the respective uptake and transport mechanisms of differently labeled ~(109)AgNPs and ~(107)Ag~+ ions. ~(109)Ag hotspots were colocalized with endosomes or lysosomes, proving an endocytosis-based entry of AgNPs which passed through the barrier of oyster gill epithelium. These ~(109)Ag hotspots showed a strong colocalization with ~(32)S~-. For the first time, we provided visualized evidence of AgNP-induced autophagy in the oyster gill cells. We further identified two categories of hemocytes (blood cells) and illustrated their roles in AgNP transport and sequestration. The integration of morphological and functional aspects of Ag subcellular distribution in different target cells suggested that oysters were equipped with a specialized endolysosomal (epithelial cells) or phagolysosomal system (hemocytes) in regulating the cellular process of AgNPs, during which the lysosome was the most involved organelle and sulfur was the most relevant macronutrient element. This study highlighted not only the intracellular but also the intercellular AgNP translocation and transformation, providing important subcellular imaging of silver and reliable methodology regarding bio-nano interactions in natural environments.

机译：银纳米粒子（AGNPS）的广泛应用需要完全检查其生物影响，特别是在Agnps可能最终的水生系统中。尽管分子量的毒性研究了各个层面，但仍然是令人生畏的挑战，以实现AG的原位亚细胞成像，并同时确定与细胞器或大分子的AgNP相互作用的位点。通过耦合高分辨率纳米级二次离子质谱法用扫描电子显微镜超结构表征的元素映射，我们成功地看来了牡蛎鳃丝中Agnps的亚细胞定位和潜在的毒性作用。还采用稳定的同位素跟踪方法研究不同标记的〜（109）AgNP和〜（107）Ag〜+离子的各自摄取和运输机制。〜（109）Ag热点用内体或溶酶体分开，证明基于内吞作用的agnps进入，该agnps通过牡蛎鳃上皮的屏障通过。这些〜（109）AG热点与〜（32）S〜 - 〜 - 。我们首次提供了AgNP诱导的oyster鳃细胞的可视化证据。我们进一步确定了两类血细胞（血细胞），并在agnp运输和封存中说明了它们的作用。在不同靶细胞中的Ag亚细胞分布的形态学和功能方面的整合表明，牡蛎配备了专门的内氨糖体（上皮细胞）或吞噬细胞）调节agnps的细胞过程，在此期间是最涉及的溶酶体细胞器和硫是最相关的Macronurient元素。该研究不仅突出了细胞内，而且突出了细胞间AgNP易位和转化，提供了关于自然环境中的生物纳米相互作用的银和可靠方法的重要亚细胞成像。

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来源
《Environmental Science & Technology》 |2021年第1期|433-446|共14页
作者
Zishuang Shao; Paul Guagliardo; Haibo Jiang; Wen-Xiong Wang;
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作者单位

Department of Ocean Science and Hong Kong Branch of the Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) The Hong Kong University of Science and Technology Kowloon Hong Kong China School of Energy and Environment and State Key Laboratory of Marine Pollution City University of Hong Kong Kowloon Hong Kong China;

Centre for Microscopy Characterisation and Analysis University of Western Australia Perth Western Australia 6009 Australia;

School of Molecular Sciences University of Western Australia Perth Western Australia 6009 Australia;

School of Energy and Environment State Key Laboratory of Marine Pollution and Hong Kong Branch of the Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) City University of Hong Kong Kowloon Hong Kong China Research Centre for the Oceans and Human Health City University of Hong Kong Shenzhen Research Institute Shenzhen 518057 China;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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Intra- and Intercellular Silver Nanoparticle Translocation and Transformation in Oyster Gill Filaments: Coupling Nanoscale Secondary Ion Mass Spectrometry and Dual Stable Isotope Tracing Study

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