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首页> 外文期刊>Environmental Science & Technology >Wavelength Dependent UV Inactivation and DNA Damage of Adenovirus as Measured by Cell Culture Infectivity and Long Range Quantitative PCR
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Wavelength Dependent UV Inactivation and DNA Damage of Adenovirus as Measured by Cell Culture Infectivity and Long Range Quantitative PCR

机译：通过细胞培养物感染性和长距离定量PCR测定的腺病毒的波长依赖性紫外线灭活和DNA损伤

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摘要

Adenovirus is regarded as the most resistant pathogen to ultraviolet (UV) disinfection due to its demonstrated resistance to monochromatic, low-pressure (LP) UV irradiation at 254 run. This resistance has resulted in high UV dose requirements for all viruses in regulations set by the United States Environmental Protection Agency. Polychromatic, medium-pressure (MP) UV irradiation has been shown to be much more effective than 254 nm, although the mechanisms of polychromatic UV inactivation are not completely understood. This research analyzes the wavelength-specific effects of UV light on adenovirus type 2 by analyzing in parallel the reduction in viral infectivity and damage to the viral genome. A tunable laser from the National Institute of Standards and Technology was used to isolate single UV wavelengths. Cell culture infectivity and PCR were employed to quantify the adenoviral inactivation rates using narrow bands of irradiation (＜1 nm) at 10 nm intervals between 210 and 290 nm. The inactivation rate corresponding to adenoviral genome damage matched the inactivation rate of adenovirus infectivity at 253.7 nm, 270 nm, 280 nm, and 290 nm, suggesting that damage to the viral DNA was primarily responsible for loss of infectivity at those wavelengths. At 260 nm, more damage to the nucleic acid was observed than reduction in viral infectivity. At 240 nm and below, the reduction of viral infectivity was significantly greater than the reduction of DNA amplification, suggesting that UV damage to a viral component other than DNA contributed to the loss of infectivity at those wavelengths. Inactivation rates were used to develop a detailed spectral sensitivity or action spectrum of adenovirus 2. This research has significant implications for the water treatment industry with regard to polychromatic inactivation of viruses and the development of novel wavelength-specific UV disinfection technologies.
机译：腺病毒被认为对紫外线（UV）消毒最具抵抗力，因为它在254运行时表现出对单色低压（LP）紫外线辐射的抵抗力。根据美国环境保护署制定的法规，这种抗性导致所有病毒的紫外线剂量要求都很高。尽管尚不完全了解多色UV灭活的机理，但已证明多色中压（MP）UV辐照比254 nm有效得多。这项研究通过并行分析病毒感染性的降低和病毒基因组的损伤，分析了紫外线对2型腺病毒的波长特异性作用。美国国家标准技术研究所的可调激光器用于隔离单个UV波长。使用细胞培养物的感染性和PCR技术，以窄的辐射带（＜1 nm）以210 nm和290 nm之间的10 nm间隔定量腺病毒的灭活率。对应于腺病毒基因组损伤的失活率与腺病毒感染性在253.7 nm，270 nm，280 nm和290 nm处的失活率相匹配，表明病毒DNA的损伤是造成这些波长下感染力丧失的主要原因。在260nm处，观察到对核酸的损害大于病毒感染性的降低。在240 nm及以下，病毒感染性的降低明显大于DNA扩增的降低，这表明紫外线对DNA以外的病毒成分的损害导致了在这些波长下感染性的丧失。灭活率被用于开发腺病毒2的详细光谱灵敏度或作用谱。这项研究对于水的多色灭活和新型波长特异性UV消毒技术的发展对水处理行业具有重要意义。

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  • 来源
    《Environmental Science & Technology》 |2014年第1期|591-598|共8页
  • 作者

    Sara E. Beck; Roberto A. Rodriguez; Karl G. Linden; Thomas M. Hargy; Thomas C. Larason; Harold B. Wright;

  • 作者单位

    Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, UCB 428, Boulder, Colorado 80309, United States;

    Environmental and Occupational Health Sciences, School of Public Health - El Paso Regional Campus, University of Texas-Health Science Center at Houston, 1101 N. Campbell St., Room 414, El Paso, Texas 79902, United States;

    Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, UCB 428, Boulder, Colorado 80309, United States;

    Tetra Tech Clancy Environmental, 20 Mapleville Depot, Saint Albans, Vermont 05478, United States;

    National Institute of Standards and Technology (NIST), 100 Bureau Dr., Gaithersburg, Maryland 20899, United States;

    Carollo Engineers, 12592 West Explorer, Suite 200, Boise, Idaho, 83713, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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