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首页> 外文期刊>Environmental Science & Technology >Environmental Conditions Influence eDNA Persistence in Aquatic Systems
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Environmental Conditions Influence eDNA Persistence in Aquatic Systems

机译：环境条件影响水生系统中eDNA的持久性

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摘要

Environmental DNA (eDNA) surveillance holds great promise for improving species conservation and management. However, few studies have investigated eDNA dynamics under natural conditions, and interpretations of eDNA surveillance results are clouded by uncertainties about eDNA degradation. We conducted a literature review to assess current understanding of eDNA degradation in aquatic systems and an experiment exploring how environmental conditions can influence eDNA degradation. Previous studies have reported macrobial eDNA persistence ranging from less than 1 day to over 2 weeks, with no attempts to quantify factors affecting degradation. Using a SYBR Green quantitative PCR assay to observe Common Carp (Cyprinus carpio) eDNA degradation in laboratory mesocosms, our rate of Common Carp eDNA detection decreased over time. Common Carp eDNA concentration followed a pattern of exponential decay, and observed decay rates exceeded previously published values for aquatic macrobial eDNA. Contrary to our expectations, eDNA degradation rate declined as biochemical oxygen demand, chlorophyll, and total eDNA (i.e., from any organism) concentration increased. Our results help explain the widely divergent, previously published estimates for eDNA degradation. Measurements of local environmental conditions, consideration of environmental influence on eDNA detection, and quantification of local eDNA degradation rates will help interpret future eDNA surveillance results.
机译：环境DNA（eDNA）监测在改善物种保护和管理方面具有广阔的前景。但是，很少有研究调查自然条件下的eDNA动力学，并且由于对eDNA降解的不确定性，对eDNA监测结果的解释模糊不清。我们进行了文献综述，以评估当前对水生系统中eDNA降解的理解，并进行了探索环境条件如何影响eDNA降解的实验。先前的研究报告了微生物eDNA的持久性，范围从不到1天到超过2周，没有尝试量化影响降解的因素。使用SYBR Green定量PCR分析法观察实验室中膜的鲤鱼eCDNA降解，我们的鲤鱼eDNA检测率随时间下降。鲤鱼eDNA的浓度遵循指数衰减的模式，并且观察到的衰减率超过了先前发布的水生微生物eDNA的值。与我们的预期相反，随着生化需氧量，叶绿素和总eDNA（即来自任何生物体）的浓度增加，eDNA降解率下降。我们的结果有助于解释先前发表的有关eDNA降解的估计差异很大。测量当地环境条件，考虑环境对eDNA检测的影响以及对当地eDNA降解率的量化将有助于解释未来的eDNA监测结果。

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  • 来源
    《Environmental Science & Technology》 |2014年第3期|1819-1827|共9页
  • 作者

    Matthew A. Barnes; Cameron R. Turner; Christopher L. Jerde; Mark A. Renshaw; W. Lindsay Chadderton; David M. Lodge;

  • 作者单位

    Department of Biological Sciences and Environmental Change Initiative, University of Notre Dame, Notre Dame, Indiana 46556, United States;

    Department of Biological Sciences and Environmental Change Initiative, University of Notre Dame, Notre Dame, Indiana 46556, United States;

    Department of Biological Sciences and Environmental Change Initiative, University of Notre Dame, Notre Dame, Indiana 46556, United States;

    Department of Biological Sciences and Environmental Change Initiative, University of Notre Dame, Notre Dame, Indiana 46556, United States;

    The Nature Conservancy, c/o Notre Dame Environmental Change Initiative, Unit 117, 1400 East Angela Boulevard, South Bend, Indiana 46617, United States;

    Department of Biological Sciences and Environmental Change Initiative, University of Notre Dame, Notre Dame, Indiana 46556, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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