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首页> 外文期刊>Environmental Science & Technology >Transcriptomic Effects-Based Monitoring for Endocrine Active Chemicals: Assessing Relative Contribution of Treated Wastewater to Downstream Pollution
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Transcriptomic Effects-Based Monitoring for Endocrine Active Chemicals: Assessing Relative Contribution of Treated Wastewater to Downstream Pollution

机译：基于转录组效应的内分泌活性化学物质监测：评估处理后的废水对下游污染的相对贡献

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摘要

The present study investigated whether a combination of targeted analytical chemistry information with unsupervised, data-rich biological methodology (Le., transcriptomics) could be utilized to evaluate relative contributions of wastewater treatment plant (WWTP) effluents to biological effects. The effects of WWTP effluents on fish exposed to ambient, receiving waters were studied at three locations with distinct WWTP and watershed characteristics. At each location, 4 d exposures of male fathead minnows to the WWTP effluent and upstream and downstream ambient waters were conducted. Transcriptomic analyses were performed on livers using 15 000 feature microarrays, foDowed by a canonical pathway and gene set enrichment analyses. Enrichment of gene sets indicative of teleost brain-pituitary-gonadal-hepatic (BPGH) axis function indicated that WWTPs serve as an important source of endocrine active chemicals (EACs) that affect the BPGH axis (e.g., cholesterol and steroid metabolism were altered). The results indicated that transcriptomics may even pinpoint pertinent adverse outcomes (Le., liver vacuolization) and groups of chemicals that preselected chemical analytes may miss. Transcriptomic Effects-Based monitoring was capable of distinguishing sites, and it reflected chemical pollution gradients, thus holding promise for assessment of relative contributions of point sources to pollution and the efficacy of pollution remediation.
机译：本研究调查了是否可以将目标分析化学信息与无监督，数据丰富的生物学方法（例如转录组学）相结合来评估废水处理厂（WWTP）废水对生物效应的相对贡献。在三个具有不同污水处理厂和分水岭特征的地点研究了污水处理厂废水对暴露于环境，接收水域的鱼类的影响。在每个地点，对雄性黑头min鱼进行了4 d暴露于污水处理厂废水以及上游和下游环境水。使用经典的通路和基因集富集分析，使用15 000个特征微阵列对肝脏进行了转录组学分析。指示硬骨脑-垂体-性腺-肝（BPGH）轴功能的基因集的富集表明，WWTP是影响BPGH轴（例如，胆固醇和类固醇代谢发生改变）的内分泌活性化学物质（EAC）的重要来源。结果表明，转录组学甚至可以查明相关的不良结局（例如，肝空泡），并且预先选择化学分析物的化学物质组可能会漏掉。基于转录组学的监测能够区分位点，并且能够反映化学污染梯度，因此有望评估点源对污染的相对贡献以及污染修复的效果。

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  • 来源
    《Environmental Science & Technology》 |2014年第4期|2385-2394|共10页
  • 作者

    Dalma Martinovic-Weigelt; Alvine C. Mehinto; Gerald T. Ankley; Nancy D. Denslow; Larry B. Barber; Kathy E. Lee; Ryan J. King; Heiko L. Schoenfuss; Anthony L. Schroeder; Daniel L. Villeneuve;

  • 作者单位

    University of St. Thomas, Department of Biology, Mail OWS 390, 2115 Summit Ave., Saint Paul, Minnesota 55105, United States;

    Southern California Coastal Water Research Project, Costa Mesa, California 92626, United States;

    U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Duluth, Minnesota 55804, United States;

    Department of Physiological Sciences, University of Florida, Gainesville, Florida 32611, United States;

    U.S. Geological Survey, Branch of Regional Research, Boulder, Colorado 80303, United States;

    U.S. Geological Survey, Water Mission Area, Mounds View, Minnesota 55744, United States;

    University of St. Thomas, Department of Biology, Mail OWS 390, 2115 Summit Ave., Saint Paul, Minnesota 55105, United States;

    Department of Biological Sciences, St. Cloud State University, St. Cloud, Minnesota 56301, United States;

    U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Duluth, Minnesota 55804, United States;

    U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Duluth, Minnesota 55804, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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