Using Transcriptomic Tools to Evaluate Biological Effects Across Effluent Gradients at a Diverse Set of Study Sites in Minnesota, USA

Jason P. Berninger; Dalma Martinovic-Weigelt; Natalia Garcia-Reyero; Lynn Escalon; Edward J. Perkins; Gerald T. Ankley; Daniel L. Villeneuve

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Using Transcriptomic Tools to Evaluate Biological Effects Across Effluent Gradients at a Diverse Set of Study Sites in Minnesota, USA

机译：在美国明尼苏达州不同地点的研究中，使用转录组学工具评估各种梯度废水的生物效应

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The aim of this study was to explore the utility of "omics" approaches in monitoring aquatic environments where complex, often unknown stressors make chemical-specific risk assessment untenable. We examined changes in the fathead minnow (Pimephales promelas) ovarian transcriptome following 4-day exposures conducted at three sites in Minnesota (MN, USA). Within each site, fish were exposed to water from three locations along a spatial gradient relative to a wastewater treatment plant (WWTP) discharge. After exposure, site-specific impacts on gene expression in ovaries were assessed. Using an intragradient point of comparison, biological responses specifically associated with the WWTP effluent were identified using functional enrichment analyses. Fish exposed to water from locations downstream of the effluent discharges exhibited many transcriptomic responses in common with those exposed to the effluent, indicating that effects of the discharge do not fully dissipate downstream. Functional analyses showed a range of biological pathways impacted through effluent exposure at all three sites. Several of those impacted pathways at each site could be linked to potential adverse reproductive outcomes associated with the hypothalamic-pituitary-gonadal (HPG) axis in female fathead minnows, specifically signaling pathways associated with oocyte meiosis, TGF-beta signaling, gonadotropin-releasing hormone (GnRH) and epidermal growth factor receptor family (ErbB), and gene sets associated with cyclin B-1 and metalloproteinase. The utility of this approach comes from the ability to identify biological responses to pollutant exposure, particularly those that can be tied to adverse outcomes at the population level and those that identify molecular targets for future studies.

机译：这项研究的目的是探索“组学”方法在监测水生环境中的效用，在这些环境中，复杂的，常常是未知的应激源使化学特异性风险评估难以进行。我们在明尼苏达州（美国明尼苏达州）的三个地点进行了4天的暴露后，研究了黑头head鱼（Pimephales promelas）卵巢转录组的变化。在每个站点内，相对于废水处理厂（WWTP）排放，鱼沿着空间梯度从三个位置暴露于水。暴露后，评估对卵巢中基因表达的位点特异性影响。使用梯度内的比较点，使用功能富集分析确定了与污水处理厂废水特别相关的生物学反应。暴露于废水排放下游位置的水中的鱼表现出许多转录组反应，与暴露于废水的鱼类相同，这表明排放的影响并未完全消散到下游。功能分析表明，所有三个地点的污水暴露都影响了一系列生物途径。每个位点的这些受影响途径中的几种可能与雌性黑头min鱼下丘脑-垂体-性腺（HPG）轴相关的潜在不良生殖结果有关，特别是与卵母细胞减数分裂，TGF-β信号传导，促性腺激素释放激素关联的信号传导途径。（GnRH）和表皮生长因子受体家族（ErbB），以及与细胞周期蛋白B-1和金属蛋白酶相关的基因集。这种方法的实用性来自于确定对污染物暴露的生物反应的能力，尤其是那些可能与人口水平的不良结果相关的反应以及为未来研究确定分子目标的反应。

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《Environmental Science & Technology》 |2014年第4期|2404-2412|共9页
作者
Jason P. Berninger; Dalma Martinovic-Weigelt; Natalia Garcia-Reyero; Lynn Escalon; Edward J. Perkins; Gerald T. Ankley; Daniel L. Villeneuve;
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作者单位

National Research Council, U.S. Environmental Protection Agency, 6201 Congdon Blvd., Duluth, Minnesota 55804, United States;

University of St. Thomas, St. Paul, Minnesota 55105, United States;

Institute of Genomics, Biocomputing, and Biotechnology, Mississippi State University, Starkville, Mississippi 39762, United States;

U.S. Army Engineer Research and Development Center, 3909 Halls Ferry Rd., Vicksburg, Mississippi 39180, United States;

U.S. Army Engineer Research and Development Center, 3909 Halls Ferry Rd., Vicksburg, Mississippi 39180, United States;

Mid-Continent Ecology Division, U.S. Environmental Protection Agency, 6201 Congdon Blvd., Duluth, Minnesota 55804, United States;

Mid-Continent Ecology Division, U.S. Environmental Protection Agency, 6201 Congdon Blvd., Duluth, Minnesota 55804, United States;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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Using Transcriptomic Tools to Evaluate Biological Effects Across Effluent Gradients at a Diverse Set of Study Sites in Minnesota, USA

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