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首页> 外文期刊>Environmental Science & Technology >Simple Whole-Cell Biodetection and Bioremediation of Heavy Metals Based on an Engineered Lead-Specific Operon
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Simple Whole-Cell Biodetection and Bioremediation of Heavy Metals Based on an Engineered Lead-Specific Operon

机译:基于工程化铅专用操纵子的重金属简单全细胞生物检测和生物修复

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摘要

A lead-specific binding protein, PbrR, and promoter pbr from the lead resistance operon, pbr, of Cupriavidus metallidurans CH34 was incorporated into E. coli in conjunction with an engineered downstream RFP (red fluorescence protein), which allowed for highly sensitive and selective whole-cell detection of lead ions. The subsequent display of PbrR on the E. coli cell surface permitted selective adsorption of lead ions from solution containing various heavy metal ions. The surface-engineered E. coli bacteria effectively protected Arabidopsis thaliana seed germination from the toxicity of lead ions at high concentrations. Engineering the E. coli bacteria harboring these lead-specific elements from the pbr operon may potentially be a valuable general strategy for biodetection and bioremediation of toxic heavy metal ions in the environment.
机译:将金属铜杯形铜尿杆菌CH34的铅抗性操纵子pbr的铅特异性结合蛋白PbrR和启动子pbr与工程改造的下游RFP(红色荧光蛋白)结合到大肠杆菌中,从而实现高度敏感和选择性铅离子的全细胞检测。随后在大肠杆菌细胞表面显示PbrR,可以从含有各种重金属离子的溶液中选择性吸附铅离子。经表面工程改造的大肠杆菌有效地保护了拟南芥种子的发芽,使其免受高浓度铅离子的毒性。工程化从pbr操纵子中携带这些铅特定元素的大肠杆菌可能是对环境中有毒重金属离子进行生物检测和生物修复的有价值的一般策略。

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  • 来源
    《Environmental Science & Technology》 |2014年第6期|3363-3371|共9页
  • 作者单位

    Institute of Chemistry and BioMedical Science, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, 210093, People's Republic of China;

    Institute of Chemistry and BioMedical Science, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, 210093, People's Republic of China;

    Institute of Chemistry and BioMedical Science, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, 210093, People's Republic of China;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210046, People's Republic of China;

    Institute of Botany, Jiangsu Province and Chinese Academy of Science, Nanjing 210018, People's Republic of China;

    Shenzhen Key Lab of Nano-Micro Material Research, Key Laboratory of Chemical Genomics, Shenzhen Graduate School of Peking University, Shenzhen, 518055, People's Republic of China;

    MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China;

    Institute of Chemistry and BioMedical Science, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, 210093, People's Republic of China ,Shenzhen Key Lab of Nano-Micro Material Research, Key Laboratory of Chemical Genomics, Shenzhen Graduate School of Peking University, Shenzhen, 518055, People's Republic of China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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