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首页> 外文期刊>Environmental Science & Technology >Gene Expression Differences between Noccaea caerulescens Ecotypes Help to Identify Candidate Genes for Metal Phytoremediation
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Gene Expression Differences between Noccaea caerulescens Ecotypes Help to Identify Candidate Genes for Metal Phytoremediation

机译:Noccaea caerulescens生态型之间的基因表达差异有助于鉴定用于金属植物修复的候选基因

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摘要

Populations of Noccaea caerulescens show tremendous differences in their capacity to hyperaccumulate and hypertolerate metals. To explore the differences that could contribute to these traits, we undertook SOLiD high-throughput sequencing of the root transcriptomes of three phenotypically well-characterized N. caerulescens accessions, i.e., Ganges, La Calamine, and Monte Prinzera. Genes with possible contribution to zinc, cadmium, and nickel hyper-accumulation and hypertolerance were predicted. The most significant differences between the accessions were related to metal ion (di-, trivalent inorganic cation) transmembrane transporter activity, iron and calcium ion binding, (inorganic) anion transmembrane transporter activity, and antioxidant activity. Analysis of correlation between the expression profile of each gene and the metal-related characteristics of the accessions disclosed both previously characterized (HMA4, HMA3) and new candidate genes (e.g., for nickel IRT1, ZIP10, and PDF2.3) as possible contributors to the hyperaccumulation/tolerance phenotype. A number of unknown Noccaea-specific transcripts also showed correlation with Zn~(2+), Cd~(2+), or Ni~(2+) hyperaccumulation/tolerance. This study shows that N. caerulescens populations have evolved great diversity in the expression of metal-related genes, facilitating adaptation to various metalliferous soils. The information will be helpful in the development of improved plants for metal phytoremediation.
机译:夜蛾Noccaea caerulescens的种群在超积累和超耐受金属的能力方面显示出巨大差异。为了探索可能导致这些性状的差异,我们对三种表型特征良好的芥蓝猪笼草(N. caerulescens)品种,即恒河,拉卡勒密和蒙特·普伦塞拉进行了根转录组的根转录组的SOLiD高通量测序。预测可能对锌,镉和镍的过度积累和超耐受性起作用的基因。这些种质之间最显着的差异与金属离子(二价,三价无机阳离子)跨膜转运蛋白活性,铁和钙离子结合,(无机)阴离子跨膜转运蛋白活性和抗氧化活性有关。分析每个基因的表达谱与先前披露的特征(HMA4,HMA3)和新的候选基因(例如,针对镍IRT1,ZIP10和PDF2.3)的候选基因的金属相关特性之间的相关性超积累/耐受表型。许多未知的夜蛾特异性转录物也显示与Zn〜(2 +),Cd〜(2+)或Ni〜(2+)的超积累/耐性相关。这项研究表明,芥蓝菌种群在金属相关基因的表达方面已发展出极大的多样性,从而有助于适应各种含金属的土壤。这些信息将有助于开发用于金属植物修复的改良植物。

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  • 来源
    《Environmental Science & Technology》 |2014年第6期|3344-3353|共10页
  • 作者单位

    Department of Biology, University of Eastern Finland, P.O. Box 1627, Kuopio, 70210, Finland;

    Laboratory of Genetics, Wageningen University, P.O. Box 309, Wageningen, 6700 AH, The Netherlands;

    Turku Centre for Biotechnology, University of Turku and Abo Akademi University, Tykistoekatu 6, Turku, 20520, Finland;

    Turku Centre for Biotechnology, University of Turku and Abo Akademi University, Tykistoekatu 6, Turku, 20520, Finland;

    Department of Plant Physiology, University of Bayreuth, Universitaetsstrasse 30, Bayreuth, D-95440, Germany;

    Department of Biology, University of Eastern Finland, P.O. Box 1627, Kuopio, 70210, Finland;

    Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, Kuopio, 70210, Finland;

    Turku Centre for Biotechnology, University of Turku and Abo Akademi University, Tykistoekatu 6, Turku, 20520, Finland;

    Department of Biology, University of Eastern Finland, P.O. Box 1627, Kuopio, 70210, Finland;

    Laboratory of Genetics, Wageningen University, P.O. Box 309, Wageningen, 6700 AH, The Netherlands;

    Department of Biology, University of Eastern Finland, P.O. Box 1627, Kuopio, 70210, Finland;

    Department of Genetics, Vrije Universiteit Amsterdam, De Boelelaan 1085, Amsterdam, 1081 HV, Netherlands;

    Department of Plant Physiology, University of Bayreuth, Universitaetsstrasse 30, Bayreuth, D-95440, Germany;

    Turku Centre for Biotechnology, University of Turku and Abo Akademi University, Tykistoekatu 6, Turku, 20520, Finland;

    Turku Centre for Biotechnology, University of Turku and Abo Akademi University, Tykistoekatu 6, Turku, 20520, Finland;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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