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首页> 外文期刊>Environmental Science & Technology >Dissolved and Particulate Copper Exposure Induces Differing Gene Expression Profiles and Mechanisms of Toxicity in the Deposit Feeding Amphipod Melita plumulosa
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Dissolved and Particulate Copper Exposure Induces Differing Gene Expression Profiles and Mechanisms of Toxicity in the Deposit Feeding Amphipod Melita plumulosa

机译：溶解和颗粒化的铜暴露诱导不同的基因表达谱和在饲料喂养的两栖类蜜蜂中的毒性机制。

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摘要

Uptake of metals via ingestion is an important route of exposure for many invertebrates, and it has been suggested that the toxic response to metals accumulated via food differs from that of metals accumulated via the dissolved phase. To test this hypothesis, the deposit-feeding epibenthic amphipod Melita plumulosa was exposed to nontoxic or reproductively toxic concentrations of copper via the overlying water, via ingestion of sediment, or via a combination of the two. Rates of copper uptake from the two exposure routes were predicted using a biokinetic model. Gene expression profiles were measured via microarray analysis and confirmed via quantitative polymerase chain reaction. Differences in expression profiles were related to the exposure route more than to individual or combined rates of copper uptake. Chitinase and digestive protease transcript expression levels correlated to the copper uptake rate from sediment, rather than from the dissolved phase or combined total uptake rate. Overall, this study supports the hypothesis that metals accumulated via ingestion have a different mode of toxic action than metals taken up from water. Consequently, guidelines that only consider dissolved metal exposure, including equilibrium-partitioning-based guidelines, may underestimate the potential effects from deposited or resuspended metal-contaminated sediments.
机译：通过摄入摄取金属是许多无脊椎动物接触的重要途径，并且已经表明，对通过食物积累的金属的毒性反应与通过溶解相积累的金属的毒性反应不同。为了检验这一假设，通过上层水，食入沉积物或通过两者的结合，将以沉积物为食的表皮两栖动物蜜ita（Melita plumulosa）暴露于无毒或生殖毒性浓度的铜中。使用生物动力学模型预测了两种暴露途径的铜吸收率。基因表达谱通过微阵列分析测量，并通过定量聚合酶链反应确认。表达谱的差异与暴露途径的关系更多，而不是与单个或联合的铜摄取率有关。几丁质酶和消化蛋白酶转录物的表达水平与沉积物中铜的吸收速率相关，而不是与溶解相或总的总吸收速率相关。总体而言，这项研究支持以下假设：通过摄入积累的金属与从水中吸收的金属具有不同的毒性作用方式。因此，仅考虑溶解金属暴露的准则（包括基于平衡分配的准则）可能会低估沉积或重悬浮的金属污染沉积物的潜在影响。

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  • 来源
    《Environmental Science & Technology》 |2014年第6期|3504-3512|共9页
  • 作者

    Sharon E. Hook; Hannah L. Osborn; Lisa A. Golding; David A. Spadaro; Stuart L. Simpson;

  • 作者单位

    Centre for Environmental Contaminants Research, CSIRO Land and Water, Locked Bag 2007, Kirrawee, New South Wales 2232, Australia;

    Centre for Environmental Contaminants Research, CSIRO Land and Water, Locked Bag 2007, Kirrawee, New South Wales 2232, Australia;

    Centre for Environmental Contaminants Research, CSIRO Land and Water, Locked Bag 2007, Kirrawee, New South Wales 2232, Australia;

    Centre for Environmental Contaminants Research, CSIRO Land and Water, Locked Bag 2007, Kirrawee, New South Wales 2232, Australia;

    Centre for Environmental Contaminants Research, CSIRO Land and Water, Locked Bag 2007, Kirrawee, New South Wales 2232, Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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