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首页> 外文期刊>Environmental Science & Technology >Genome-Wide Transcription Profiles Reveal Genotype-Dependent Responses of Biological Pathways and Gene-Families in Daphnia Exposed to Single and Mixed Stressors
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Genome-Wide Transcription Profiles Reveal Genotype-Dependent Responses of Biological Pathways and Gene-Families in Daphnia Exposed to Single and Mixed Stressors

机译：全基因组转录谱揭示了暴露于单一和混合应激源的水蚤的生物途径和基因家族的基因型依赖性反应。

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摘要

The present study investigated the possibilities and limitations of implementing a genome-wide transcription-based approach that takes into account genetic and environmental variation to better understand the response of natural populations to stressors. When exposing two different Daphnia pulex genotypes (a cadmium-sensitive and a cadmium-tolerant one) to cadmium, the toxic cyanobacteria Microcystis aeruginosa, and their mixture, we found that observations at the transcriptomic level do not always explain observations at a higher level (growth, reproduction). For example, although cadmium elicited an adverse effect at the organismal level, almost no genes were differentially expressed after cadmium exposure. In addition, we identified oxidative stress and polyunsaturated fatty acid metabolism-related pathways, as well as trypsin and neurexin IV gene-families as candidates for the underlying causes of genotypic differences in tolerance to Microcystis. Furthermore, the whole-genome transcriptomic data of a stressor mixture allowed a better understanding of mixture responses by evaluating interactions between two stressors at the gene-expression level against the independent action baseline model. This approach has indicated that ubiquinone pathway and the MAPK serine-threonine protein kwase and collagens gene-families were enriched with genes showing an interactive effect in expression response to exposure to the mixture of the stressors, while transcription and translation-related pathways and gene-families were mostly related with genotypic differences in interactive responses to this mixture. Collectively, our results indicate that the methods we employed may improve further characterization of the possibilities and limitations of transcriptomics approaches in the adverse outcome pathway framework and in predictions of multistressor effects on natural populations.
机译：本研究调查了实现全基因组基于转录的方法的可能性和局限性，该方法考虑了遗传和环境变异，以更好地了解自然种群对应激源的反应。当将有毒蓝藻铜绿微囊藻及其混合物暴露于镉的两种不同的水蚤基因型（对镉敏感和对镉耐受的一种）和镉的混合物时，我们发现转录组水平的观察结果并不能总解释较高水平的观察结果（生长，繁殖）。例如，尽管镉在机体水平上引起了不利影响，但是镉暴露后几乎没有差异表达的基因。此外，我们确定了氧化应激和多不饱和脂肪酸代谢相关的途径，以及胰蛋白酶和神经毒素IV基因家族可作为对微囊藻耐受性基因型差异的潜在原因。此外，应激源混合物的全基因组转录组数据通过对照独立作用基线模型评估基因表达水平上两个应激源之间的相互作用，可以更好地理解混合物的反应。该方法表明，泛醌途径以及MAPK丝氨酸-苏氨酸蛋白水解酶和胶原蛋白基因家族富含基因，这些基因在暴露于应激源的混合物中对表达反应具有交互作用，而转录和翻译相关的途径以及与家庭对这种混合物的交互反应主要与基因型差异有关。总体而言，我们的结果表明，我们采用的方法可能会进一步改善转录组学方法在不良结局途径框架中以及在对自然种群的多重应激影响预测中的可能性和局限性。

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  • 来源
    《Environmental Science & Technology》 |2014年第6期|3513-3522|共10页
  • 作者

    Dieter I. M. De Coninck; Jana Asselman; Stephen Glaholt; Colin R. Janssen; John K. Colbourne; Joseph R. Shaw; Karel A. C. De Schamphelaere;

  • 作者单位

    Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, Gent, Belgium;

    Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, Gent, Belgium;

    School of Public & Environmental Affairs, Indiana University, Bloomington, IN, United States;

    Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, Gent, Belgium;

    Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN, United States ,School of Biosciences, University of Birmingham, Edgebaston, Birmingham, United Kingdom;

    School of Public & Environmental Affairs, Indiana University, Bloomington, IN, United States ,Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN, United States ,School of Biosciences, University of Birmingham, Edgebaston, Birmingham, United Kingdom;

    Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, Gent, Belgium;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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