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首页> 外文期刊>Environmental Science & Technology >High-Affinity Sulfate Transporter Sultr1;2 Is a Major Transporter for Cr(Ⅵ) Uptake in Plants
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High-Affinity Sulfate Transporter Sultr1;2 Is a Major Transporter for Cr(Ⅵ) Uptake in Plants

机译：高亲和力硫酸盐转运蛋白巯基; 2是Cr（Ⅵ）植物摄取的主要转运蛋白

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摘要

Chromate (Cr[Ⅵ]) is a highly phytotoxic contaminant that is ubiquitous in soils. However, how Cr(Ⅵ) is taken up by plant roots remains largely unknown. Here, we show that the high-affinity sulfate transporter Sultrl;2 is responsible for Cr(Ⅵ) uptake by the roots of Arabidopsis thaliana. Sultr1;2 showed a much higher transport activity for Cr(Ⅵ) than Sultr1;1 when expressed in yeast cells. Knockdown of Sultr1;2 expression in Arabidopsis markedly reduced the Cr(Ⅵ) uptake rate, whereas knockout of Sultr1;1 had no or little effect. A double-knockout mutant (DKO) of the two genes lost the ability of Cr(Ⅵ) uptake almost completely. The Sultr1;1 knockdown mutant or DKO plants displayed higher resistance to Cr(Ⅵ) under normal sulfate conditions as a consequence of the lower tissue Cr accumulation. Overexpression of Sultr1;2 substantially increased Cr(Ⅵ) uptake with shoot Cr concentration being 1.6-2.0 times higher than that in the wild-type. These results indicate that Sultr1;2 is a major transporter responsible for Cr(Ⅵ) uptake in Arabidopsis, while Sultrl; 1 plays a negligible role. Taken together, our study has identified a major transporter for Cr(Ⅵ) uptake in plants, providing potential strategies for engineering plants with low Cr accumulation and consequently enhanced Cr(Ⅵ) resistance and also plants with enhanced accumulation of Cr for the purpose of phytoremediation.
机译：铬酸盐（Cr [ⅵ]）是一种高植物毒性污染物，其在土壤中普遍存在。然而，植物根部占用Cr（ⅵ）仍然很大程度上未知。在这里，我们表明高亲和力硫酸盐转运蛋白; 2是由拟南芥根系的Cr（ⅵ）吸收的负责。 Sultr1; 2表现出在酵母细胞中表达时的Cr（ⅵ）的较高的rel1; 1。抑制巯珠1; 2拟南芥的表达明显降低了Cr（Ⅵ）摄取率，而淘汰的淘汰率没有或效果不大。两种基因的双敲除突变体（DKO）几乎完全失去了Cr（ⅵ）摄取的能力。由于较低的组织Cr积累，巯基突变体或DKO植物在正常硫酸盐条件下显示出较高的Cr（Ⅵ）的抗性。 Sultr1的过度表达; 2基本上增加的Cr（ⅵ）摄取，射击Cr浓度比野生型高1.6-2.0倍。这些结果表明，巯基; 2是负责拟南芥的Cr（Ⅵ）摄取的主要转运蛋白，而釜族; 1发挥了可忽略不计的作用。我们的研究已经鉴定了植物中Cr（Ⅵ）摄取的主要运输术，为具有低Cr积累的工程植物提供潜在的策略，因此增强了Cr（Ⅵ）抗性，并且在植物修复的目的中具有增强的CR积累的植物。

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  • 来源
    《Environmental Science & Technology》 |2021年第3期|1576-1584|共9页
  • 作者

    Zhong-Rui Xu; Mei-Ling Cai; Si-Hong Chen; Xin-Yuan Huang; Fang-Jie Zhao; Peng Wang;

  • 作者单位

    College of Resources and Environmental Sciences and jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Nanjing Agricultural University Nanjing 210095 China;

    College of Resources and Environmental Sciences and Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Nanjing Agricultural University Nanjing 210095 China;

    College of Resources and Environmental Sciences and jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Nanjing Agricultural University Nanjing 210095 China;

    College of Resources and Environmental Sciences and jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Nanjing Agricultural University Nanjing 210095 China;

    College of Resources and Environmental Sciences and Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Nanjing Agricultural University Nanjing 210095 China;

    College of Resources and Environmental Sciences and Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Nanjing Agricultural University Nanjing 210095 China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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