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首页> 外文期刊>Environmental Science & Technology >Hidden Resistome: Enrichment Reveals the Presence of Clinically Relevant Antibiotic Resistance Determinants in Treated Wastewater-Irrigated Soils
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Hidden Resistome: Enrichment Reveals the Presence of Clinically Relevant Antibiotic Resistance Determinants in Treated Wastewater-Irrigated Soils

机译：隐藏的抵抗数：富集揭示治疗废水灌溉土壤中临床相关抗生素抗性决定簇的存在

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摘要

Treated-wastewater (TW) irrigation transfers antibiotic-resistant bacteria (ARB) to soil, but persistence of these bacteria is generally low due to resilience of the soil microbiome. Nonetheless, wastewater-derived bacteria and associated antibiotic resistance genes (ARGs) may persist below detection levels and potentially proliferate under copiotrophic conditions. To test this hypothesis, we exposed soils from microcosm, lysimeter, and field experiments to short-term enrichment in copiotroph-stimulating media. In microcosms, enrichment stimulated growth of multidrug-resistant Escherichia coli up to 2 weeks alter falling below detection limits. Lysimeter and orchard soils irrigated in-tandem with either freshwater or TW were subjected to culture-based, qPCR and shotgun metagenomic analyses prior,and subsequent,toenrichment. Although native TW- and freshwater-irrigated soil microbiomes and resistomes were similar to each other, enrichment resulted in higher abundances of cephalosporin- and carbapenem-resistant Enterobacteriaceae and in substantial differences in the composition of microbial communities and ARGs. Enrichment stimulated ARG-harboring Bacillaceae in the freshwater-irrigated soils,whereasinTWW-irrigatedsoils,ARG-harboring γ-proteobacterialfamiliesEnter obacteriaceaeand Moraxellaceae were more profuse. We demonstrate that TW-derived ARB and associated ARGs can persist at below detection levels in irrigated soils and believe that similar short-term enrichment strategies can be applied for environmental antimicrobial risk assessment in the future.
机译：处理废水（TW）灌溉将抗生素抗性细菌（ARB）转移到土壤中，但由于土壤微生物组的弹性，这些细菌的持久性通常是低的。尽管如此，废水衍生的细菌和相关的抗生素抗性基因（Args）可能持续低于检测水平，并且在跨营养的条件下可能增殖。为了测试这一假设，我们从微观血液刺激培养基中从微观核肉，溶血性和现场实验中暴露了土壤，以对短期富集的短期富集。在微观微观中，富集刺激多药抗性大肠杆菌的生长，最多2周变化低于检测限。淡水或TW灌溉的溶血仪和果园土壤灌溉含有淡水或TW的培养基，QPCR和霰弹枪均衡分析，以及随后的心绞痛。虽然天然的TW和淡水灌溉的土壤微生物和电阻相似，但富集导致耐高量的头孢菌素和碳癌肠癌酸痛，并且在微生物社区和args的组成中存在显着差异。富集刺激氨基硝基氏菌在淡水灌溉的土壤中，WhereAtww-ItrigateSils，arg-habledingγ-proteobacterialfamiliesenter alocakiaceand moraxellaceae更加丰富。我们证明，TW-衍生的Arb和相关的arbs可以在低于灌溉土壤的检测水平下持续存在，并且相信未来的环境抗菌风险评估可以应用类似的短期富集策略。

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  • 来源
    《Environmental Science & Technology》 |2021年第10期|6814-6827|共14页
  • 作者

    Roberto B. M. Marano; Chhedi Lai Gupta; Tamar Cozer; Edouard Jurkevitch; Eddie Cytryn;

  • 作者单位

    Department of Soil Chemistry Plant Nutrition and Microbiology Institute of Soil Water and Environmental Sciences The Volcani Center Agricultural Research Organization Rishon LeZion 7505101 Israel Department of Agroecology and Plant Health The Robert H. Smith Faculty of Agriculture Food and Environment The Hebrew University of Jerusalem Rehovot 76100 Israel;

    Department of Soil Chemistry Plant Nutrition and Microbiology Institute of Soil Water and Environmental Sciences The Volcani Center Agricultural Research Organization Rishon LeZion 7505101 Israel;

    Department of Soil Chemistry Plant Nutrition and Microbiology Institute of Soil Water and Environmental Sciences The Volcani Center Agricultural Research Organization Rishon LeZion 7505101 Israel The Mina and Everard Goodman Faculty of Life Sciences Bar-Ilan University Ramat-Gan 5290002 Israel;

    Department of Agroecology and Plant Health The Robert H. Smith Faculty of Agriculture Food and Environment The Hebrew University of Jerusalem Rehovot 76100 Israel;

    Department of Soil Chemistry Plant Nutrition and Microbiology Institute of Soil Water and Environmental Sciences The Volcani Center Agricultural Research Organization Rishon LeZion 7505101 Israel;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    treated-wastewater irrigation; antibiotic resistance; contaminants of emerging concern; soil; microbiome; resistome;

    机译：处理废水灌溉;抗生素耐药性;出现的污染物;土壤;微生物组;抵抗;

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