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首页> 外文期刊>Environmental Science & Technology >Pyrosequencing Reveals Bacterial Communities in Unchlorinated Drinking Water Distribution System: An Integral Study of Bulk Water, Suspended Solids, Loose Deposits, and Pipe Wall Biofilm
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Pyrosequencing Reveals Bacterial Communities in Unchlorinated Drinking Water Distribution System: An Integral Study of Bulk Water, Suspended Solids, Loose Deposits, and Pipe Wall Biofilm

机译:焦磷酸测序揭示了无氯饮用水分配系统中的细菌群落:大量水,悬浮固体,松动沉积物和管壁生物膜的综合研究

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摘要

The current understanding of drinking water distribution system (DWDS) microbiology is limited to pipe wall biofilm and bulk water; the contributions of particle-associated bacteria (from suspended solids and loose deposits) have long been neglected. Analyzing the composition and correlation of bacterial communities from different phases helped us to locate where most of the bacteria are and understand the interactions among these phases. In the present study, the bacteria from four critical phases of an unchlorinated DWDS, including bulk water, pipe wall biofilm, suspended solids, and loose deposits, were quantified and identified by adenosine triphosphate analysis and pyrosequencing, respectively. The results snowed that the bulk water bacteria (including the contribution of suspended solids) contributed less than 2% of the total bacteria. The bacteria associated with loose deposits and pipe wall biofilm that accumulated in the DWDS accounted for over 98% of the total bacteria, and the contributions of bacteria in loose deposits and pipe wall biofilm were comparable. Depending on the amount of loose deposits, its contribution can be 7-fold higher than the pipe wall biofilm. Pyrosequencing revealed relatively stable bacterial communities in bulk water, pipe wall biofilm, and suspended solids throughout the distribution system; however, the communities present in loose deposits were dependent on the amount of loose deposits locally. Bacteria within the phases of suspended solids, loose deposits, and pipe wall biofilm were similar in phylogenetic composition. The bulk water bacteria (dominated by Polaromonas spp.) were clearly different from the bacteria from the other three phases (dominated by Sphingommas spp.). This study highlighted that the integral DWDS ecology should include contributions from all of the four phases, especially the bacteria harbored by loose deposits. The accumulation of loose deposits and the aging process create variable microenvirorunents inside loose deposits structures for bacteria to grow. Moreover, loose deposits protect the associated bacteria from disinfectants, and due to their mobility, the associated bacteria reach taps easily.
机译:当前对饮用水分配系统(DWDS)微生物学的了解仅限于管壁生物膜和散装水;长期以来,人们一直忽略了颗粒相关细菌(来自悬浮固体和疏松沉积物)的贡献。分析不同阶段细菌群落的组成和相关性有助于我们找到大多数细菌所在的位置,并了解这些阶段之间的相互作用。在本研究中,分别通过三磷酸腺苷分析和焦磷酸测序对来自未氯化DWDS四个关键阶段的细菌进行了定量和鉴定,包括散装水,管壁生物膜,悬浮固体和疏松的沉积物。结果下雪了,主要的水细菌(包括悬浮固体的贡献)贡献不到总细菌的2%。 DWDS中累积的与疏松沉积物和管壁生物膜相关的细菌占总数的98%以上,并且细菌在疏松沉积物和管壁生物膜中的贡献相当。根据松散沉积物的数量,其贡献可能比管壁生物膜高7倍。焦磷酸测序显示散布水,管壁生物膜和整个分配系统中的悬浮固体中细菌群体​​相对稳定。但是,存在于零散存款中的社区取决于当地零散存款的数量。悬浮固体,疏松沉积物和管壁生物膜相中的细菌在系统发育组成上相似。大量的水细菌(由北极单胞菌属占主导)与其他三个阶段的细菌(由鞘氨醇单胞菌占主导)明显不同。这项研究强调,DWDS的整体生态学应该包括四个阶段的全部贡献,尤其是松散沉积物所藏藏的细菌。松散沉积物的积累和老化过程会在松散沉积物结构内部创建可变的微环境,以使细菌生长。此外,疏松的沉积物可保护相关细菌免受消毒剂的污染,并且由于它们的流动性,相关细菌很容易到达水龙头。

著录项

  • 来源
    《Environmental Science & Technology》 |2014年第10期|5467-5476|共10页
  • 作者单位

    Section Sanitary Engineering, Department of Water Management, Faculty of Civil Engineering and Geosciences, Delft University of Technology, P.O. Box 5048, 2600 GA Delft, The Netherlands;

    Vitens Water Company, P.O. Box 1205, 8001 BE Zwolle, The Netherlands;

    Section Sanitary Engineering, Department of Water Management, Faculty of Civil Engineering and Geosciences, Delft University of Technology, P.O. Box 5048, 2600 GA Delft, The Netherlands,Water Desalination and Reuse Research Center, 4700, King Abdullah University of Science and Technology, Thuwal 23955-6900,Kingdom of Saudi Arabia;

    Section Sanitary Engineering, Department of Water Management, Faculty of Civil Engineering and Geosciences, Delft University of Technology, P.O. Box 5048, 2600 GA Delft, The Netherlands,KWR Watercyde Research Institute, P.O. Box 1072, 3430BB Nieuwegein, The Netherlands;

    Section Sanitary Engineering, Department of Water Management, Faculty of Civil Engineering and Geosciences, Delft University of Technology, P.O. Box 5048, 2600 GA Delft, The Netherlands;

    Section Sanitary Engineering, Department of Water Management, Faculty of Civil Engineering and Geosciences, Delft University of Technology, P.O. Box 5048, 2600 GA Delft, The Netherlands,KWR Watercyde Research Institute, P.O. Box 1072, 3430BB Nieuwegein, The Netherlands;

    Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, 205 North Mathews Avenue, Urbana, Illinois 61801, United States;

    Section Sanitary Engineering, Department of Water Management, Faculty of Civil Engineering and Geosciences, Delft University of Technology, P.O. Box 5048, 2600 GA Delft, The Netherlands;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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