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首页> 外文期刊>Environmental Science & Technology >Small ~(13)C/~(12)C Fractionation Contrasts with Large Enantiomer Fractionation in Aerobic Biodegradation of Phenoxy Acids
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Small ~(13)C/~(12)C Fractionation Contrasts with Large Enantiomer Fractionation in Aerobic Biodegradation of Phenoxy Acids

机译：苯氧酸的好氧生物降解中小〜（13）C /〜（12）C分馏对比与大对映异构体分馏

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摘要

Phenoxy acid herbicides are important ground-water contaminants. Stable isotope analysis and enantiomer analysis are well-recognized approaches for assessing in situ biodegradation in the field. In an aerobic degradation survey with six , phenoxvacetic add and three phenoxypropionic acid-degrading bacteria we measured (a) enantiomer-specific carbon isotope fractionation of MCPP ((R,S)-2-(4-chloro-2-methylphenoxy)-propionic add), DCPP ((R,S)-2-(2,4-dichlorophenoxy)-propionic add), and 4-CPP ((R,S)-2-(4-chlorophenoxy)-propionic add); (b) compound-specific isotope fractionation of MCPA (4-chloro-2-methylphenoxyacenc add) and 2,4-D (2,4-dichlorophenoiyacenc acid); and (c) enantiomer fractionatinon of MCPP, DCPP, and 4-CPP. Insignificant or very slight (ε= -13‰ to -2.0‰) carbon isotope fractionation was observed Equally small values in an RdpA enzyme assay (ε_(ea) = -1.0 ± 0.1 ‰) and even smaller fractionation in whole cell experiments of the host organism Sphingobium herbicidovorans MH (ε_(wc) = -0.3 ± 0.1‰) suggest that (ⅰ) enzyme-associated isotope effects were already small, yet (ⅱ) further masked by active transport through the cell membrane. In contrast, enantiomer fractionation in MCPP, DCPP, and 4-CPP was pronounced, with enantioselcctivities (ES) of -0.65 to -0.98 with Sphingomonas sp. PM2, -0.63 to -0.89 with Sphmgobium herbicidovorans MH, and 0.74 to 0.97 with Delftia acidvorans MC1. To detect aerobic biodegradation of phenoxypropionic acids in the field, enantiomer fractionation seems, therefore, a stronger indicator than carbon isotope fractionation.
机译：苯氧酸除草剂是重要的地下水污染物。稳定的同位素分析和对映异构体分析是评估该领域原位生物降解的公认方法。在有氧，六苯氧和3种苯氧丙酸降解菌的有氧降解调查中，我们测量了（a）MCPP（（R，S）-2-（4-氯-2-甲基苯氧基）-丙酸的对映体特异性碳同位素分馏添加），DCPP（（R，S）-2-（2,4-二氯苯氧基）-丙酸添加）和4-CPP（（R，S）-2-（4-氯苯氧基）-丙酸添加）; （b）MCPA（4-氯-2-甲基苯氧基乙炔添加）和2,4-D（2,4-二氯苯氧杂cen酸）的化合物特异性同位素分馏； （c）MCPP，DCPP和4-CPP的对映体分数。观察到的碳同位素分馏微乎其微或非常小（ε= -13‰--2.0‰）在RdpA酶测定中的值均较小（ε_（ea）= -1.0±0.1‰），而在全细胞实验中的分馏甚至更小宿主生物除草鞘氨醇单孢菌MH（ε_（wc）= -0.3±0.1‰）表明（ⅰ）与酶相关的同位素效应已经很小，但（ⅱ）被通过细胞膜的主动转运进一步掩盖了。相比之下，MCPP，DCPP和4-CPP中的对映体分馏明显，鞘氨醇单胞菌属的对映体电势（ES）为-0.65至-0.98。 PM2，除草贪食弧菌MH为-0.63至-0.89，而酸伏特氏菌MC1为0.74至0.97。为了在现场检测苯氧丙酸的好氧生物降解，对映体分馏似乎比碳同位素分馏更强。

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  • 来源
    《Environmental Science & Technology》 |2014年第10期|5501-5511|共11页
  • 作者

    Shiran Qiu; Erkin Goezdereliler; Philip Weyrauch; Eva C. Magana Lopez; Hans-Peter E. Kohler; Sebastian R. Sorensen; Rainer U. Meckenstock; Martin Eisner;

  • 作者单位

    Institute of Groundwater Ecology, Helmholtz Zentrum Muenchen, Ingolstadter Landstr. 1, 85764 Neuherberg, Germany;

    Institute of Groundwater Ecology, Helmholtz Zentrum Muenchen, Ingolstadter Landstr. 1, 85764 Neuherberg, Germany,Department of Geochemistry, Geological Survey of Denmark and Greenland (GEUS), Oster Voldgade 10, 1350 Copenhagen K,Denmark;

    Institute of Groundwater Ecology, Helmholtz Zentrum Muenchen, Ingolstadter Landstr. 1, 85764 Neuherberg, Germany;

    Institute of Groundwater Ecology, Helmholtz Zentrum Muenchen, Ingolstadter Landstr. 1, 85764 Neuherberg, Germany;

    Swiss Federal Institute of Aquatic Science and Technology (Eawag), UEberlandstrasse 133, CH-8600 Duebendorf, Switzerland;

    Department of Geochemistry, Geological Survey of Denmark and Greenland (GEUS), Oster Voldgade 10, 1350 Copenhagen K,Denmark;

    Institute of Groundwater Ecology, Helmholtz Zentrum Muenchen, Ingolstadter Landstr. 1, 85764 Neuherberg, Germany;

    Institute of Groundwater Ecology, Helmholtz Zentrum Muenchen, Ingolstadter Landstr. 1, 85764 Neuherberg, Germany;

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