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首页> 外文期刊>Environmental Science & Technology >Sludge As a Potential Important Source of Antibiotic Resistance Genes in Both the Bacterial and Bacteriophage Fractions
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Sludge As a Potential Important Source of Antibiotic Resistance Genes in Both the Bacterial and Bacteriophage Fractions

机译：污泥是细菌和噬菌体组分中潜在的抗生素耐药基因的重要来源

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摘要

The emergence and prevalence of antibiotic resistance genes (ARGs) in the environment is a serious global health concern. ARGs found in bacteria can become mobilized in bacteriophage particles in the environment Sludge derived from secondary treatment in wastewater treatment plants (WWTPs) constitutes a concentrated pool of bacteria and phages that are removed during the treatment process. This study evaluates the prevalence of ARGs in the bacterial and phage fractions of anaerobic digested sludge; five ARGs (bla_(TEM), bla_(CTX-M), qnrA, qnrS, and sul1) are quantified by qPCR. Comparison between the wastewater and sludge revealed a shift in the prevalence of ARGs (bla_(TEM) and sul1 became more prevalent in sludge), suggesting there is a change in the bacterial and phage populations from wastewater to those selected during the secondary treatment and the later anaerobic mesophilic digestion of the sludge. ARGs densities were higher in the bacterial than in the phage fraction, with high densities in both fractions; particularly for bla_(TEM) and sul1 (5 and 8 log_(10) gene copies (GC)/g, respectively, in bacterial DNA; 5.5 and 4.4 log_(10) GC/g, respectively, in phage DNA). These results question the potential agricultural uses of treated sludge, as it could contribute to the spread of ARGs in the environment and have an impact on the bacterial communities of the receiving ecosystem.
机译：环境中抗生素抗性基因（ARG）的出现和流行是严重的全球健康问题。在细菌中发现的ARGs可以在环境中的噬菌体颗粒中动员。废水处理厂（WWTP）中二次处理产生的污泥构成了细菌和噬菌体的浓缩池，在处理过程中会被去除。这项研究评估了厌氧消化污泥中细菌和噬菌体级分中ARGs的患病率。通过qPCR对5个ARG（bla_（TEM），bla_（CTX-M），qnrA，qnrS和sul1）进行定量。废水和污泥之间的比较显示ARGs的发生率发生了变化（bla_（TEM）和sul1在污泥中变得更加普遍），这表明细菌和噬菌体的数量从废水变为了在二级处理中选择的细菌和噬菌体。以后对污泥进行厌氧中温消化。细菌中的ARGs密度高于噬菌体级分，两个级分均具有高密度。特别是bla_（TEM）和sul1（细菌DNA中分别为5和8 log_（10）基因拷贝（GC）/ g；噬菌体DNA中分别为5.5和4.4 log_（10）GC / g）。这些结果质疑处理后的污泥的潜在农业用途，因为它可能有助于ARGs在环境中的扩散，并影响接收生态系统的细菌群落。

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  • 来源
    《Environmental Science & Technology》 |2014年第13期|7602-7611|共10页
  • 作者

    William Calero-Caceres; Ana Melgarejo; Marta Colomer-Lluch; Claudia Stoll; Francisco Lucena; Juan Jofre; Maite Muniesa;

  • 作者单位

    Department of Microbiology, University of Barcelona, Diagonal 643, Annex, Floor 0, E-08028 Barcelona, Spain;

    Department of Microbiology, University of Barcelona, Diagonal 643, Annex, Floor 0, E-08028 Barcelona, Spain;

    Department of Microbiology, University of Barcelona, Diagonal 643, Annex, Floor 0, E-08028 Barcelona, Spain;

    DVGW-Technologiezentrum Wasser (TZW), Karlsruher Street 84, D-76139 Karlsruhe, Germany;

    Department of Microbiology, University of Barcelona, Diagonal 643, Annex, Floor 0, E-08028 Barcelona, Spain;

    Department of Microbiology, University of Barcelona, Diagonal 643, Annex, Floor 0, E-08028 Barcelona, Spain;

    Department of Microbiology, University of Barcelona, Diagonal 643, Annex, Floor 0, E-08028 Barcelona, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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