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首页> 外文期刊>Environmental Science & Technology >Pyrosequencing Analysis Yields Comprehensive Assessment of Microbial Communities in Pilot-Scale Two-Stage Membrane Biofilm Reactors
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Pyrosequencing Analysis Yields Comprehensive Assessment of Microbial Communities in Pilot-Scale Two-Stage Membrane Biofilm Reactors

机译：焦磷酸测序分析中试规模的两阶段膜生物膜反应器中微生物群落的综合评估。

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摘要

We studied the microbial community structure of pilot two-stage membrane biofilm reactors (MBfRs) designed to reduce nitrate (NO_3~-) and perchlorate (ClO_4~-) in contaminated groundwater. The groundwater also contained oxygen (O_2) and sulfate (SO_4~(2-)), which became important electron sinks that affected the NO_3~- and ClO_4~-removal rates. Using pyrosequencing, we elucidated how important phylotypes of each "primary" microbial group, i.e., denitrifying bacteria (DB), perchlorate-reducing bacteria (PRB), and sulfate-reducing bacteria (SRB), responded to changes in electron-acceptor loading. UniFrac, principal coordinate analysis (PCoA), and diversity analyses documented that the microbial community of biofilms sampled when the MBfRs had a high acceptor loading were phylogenetically distant from and less diverse than the microbial community of biofilm samples with lower acceptor loadings. Diminished acceptor loading led to SO_4~(2-) reduction in the lag MBfR, which allowed Desulfovibrionales (an SRB) and Thiothrichales (sulfur-oxidizers) to thrive through S cycling. As a result of this cooperative relationship, they competed effectively with DB/PRB phylotypes such as Xanthomonadales and Rhodobacterales. Thus, pyrosequencing illustrated that while DB, PRB, and SRB responded predictably to changes in acceptor loading, a decrease in total acceptor loading led to important shifts within the "primary" groups, the onset of other members (e.g., Thiothrichales), and overall greater diversity.
机译：我们研究了中试两级膜生物膜反应器（MBfRs）的微生物群落结构，该反应器旨在减少受污染的地下水中的硝酸盐（NO_3〜-）和高氯酸盐（ClO_4〜-）。地下水中还含有氧气（O_2）和硫酸盐（SO_4〜（2-）），这成为影响NO_3〜-和ClO_4〜去除速率的重要电子阱。使用焦磷酸测序，我们阐明了每个“主要”微生物组（即反硝化细菌（DB），减少高氯酸盐的细菌（PRB）和减少硫酸盐的细菌（SRB））的重要系统型对电子受体负载的变化有何响应。 UniFrac，主坐标分析（PCoA）和多样性分析表明，当MBfRs具有高受体载量时，所采样的生物膜微生物群落在系统发育上与具有较低受体载量的生物膜样品的微生物群落在系统发育上相距甚远。受体负载的减少导致滞后MBfR的SO_4〜（2-）降低，这使得脱硫弧菌（一种SRB）和硫代硫菌（硫氧化剂）通过S循环而蓬勃发展。由于这种合作关系，他们可以与Xanthomonadales和Rhodobacterales等DB / PRB系统型有效竞争。因此，焦磷酸测序表明，尽管DB，PRB和SRB对受体负荷的变化具有可预测的响应，但总受体负荷的减少导致“主要”组内其他成员（例如，Thiothrichales）的发作以及总体的重要转变。更大的多样性。

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  • 来源
    《Environmental Science & Technology》 |2014年第13期|7511-7518|共8页
  • 作者

    Aura Ontiveros-Valencia; Youneng Tang; He-Ping Zhao; David Friese; Ryan Overstreet; Jennifer Smith; Patrick Evans; Bruce E. Rittmann; Rosa Krajmalnik-Brown;

  • 作者单位

    Biodesign Institute, Swette Center for Environmental Biotechnology, Arizona State University, 1001 South McAllister Avenue, Tempe, Arizona 85287-5701, United States,School of Sustainability, Arizona State University, PO Box 875502, Tempe, Arizona 85287-5502, United States;

    Biodesign Institute, Swette Center for Environmental Biotechnology, Arizona State University, 1001 South McAllister Avenue, Tempe, Arizona 85287-5701, United States,University of Illinois at Urbana- Champaign, Urbana, IL 61801, USA;

    Biodesign Institute, Swette Center for Environmental Biotechnology, Arizona State University, 1001 South McAllister Avenue, Tempe, Arizona 85287-5701, United States,MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou, China;

    APTwater, Inc., 100 W. Broadway, Suite 200, Long Beach, California 90802, United States;

    APTwater, Inc., 100 W. Broadway, Suite 200, Long Beach, California 90802, United States;

    CDM-Smith, 14432 SE Eastgate Way, Bellevue, Washington 98007, United States;

    CDM-Smith, 14432 SE Eastgate Way, Bellevue, Washington 98007, United States;

    Biodesign Institute, Swette Center for Environmental Biotechnology, Arizona State University, 1001 South McAllister Avenue, Tempe, Arizona 85287-5701, United States;

    Biodesign Institute, Swette Center for Environmental Biotechnology, Arizona State University, 1001 South McAllister Avenue, Tempe, Arizona 85287-5701, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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