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首页> 外文期刊>Environmental Science & Technology >Use of Metallomics and Metabolomics to Assess Metal Pollution in Donana National Park (SW Spain)
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Use of Metallomics and Metabolomics to Assess Metal Pollution in Donana National Park (SW Spain)

机译：利用金属组学和代谢组学评估多纳纳国家公园的金属污染（西班牙西南部）

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摘要

Monitoring organism exposure to heavy metals has acquired increased importance in the last decades. The mouse Mus spretus has been used to assess the biological response to contaminants in the relevant ecological area of Donana National Park (DNP) and surrounding areas (SW Spain), where many migrating birds land for breeding and feeding every year. A metallomics approach, based on the characterization of metal biomolecules using size exclusion chromatography coupled with inductively coupled plasma-mass spectrometry (SEC-ICP-MS) and a metabolomics approach based on direct infusion to a mass spectrometer (DI-ESI-QTOF-MS) followed by a partial linear square-discriminant analysis (PLS-DA), were used to compare the biological responses of M. spretus living in three areas of DNP (the reference) and surrounding areas (El Partido and El Matochal). The activities of key antioxidant enzymes, such as Cu/Zn-SOD, Mn-SOD, CAT, GR, and guaiacol peroxidase, were also determined in connection with environmental contamination issue. The results show differences caused by the presence of metals in the ecosystem that affected to the levels of metals and metalloproteins, such as MT, Cu/Zn-SOD, or Mn-CA, the breakdown of membrane phospholipids, perturbations in metabolic pathways, related to energy metabolism, and oxidative stress.
机译：在过去的几十年中，监测生物体对重金属的暴露已变得越来越重要。在多纳纳国家公园（DNP）和周围地区（西班牙西南部）的相关生态区域中，老鼠mus spretus已用于评估对污染物的生物反应，那里每年都有许多迁徙鸟类在此繁殖和觅食。一种金属组学方法，基于使用尺寸排阻色谱结合电感耦合等离子体质谱法（SEC-ICP-MS）表征金属生物分子的方法，以及基于直接注入质谱仪的代谢组学方法（DI-ESI-QTOF-MS ），然后进行部分线性平方判别分析（PLS-DA），用于比较生活在DNP（参考）的三个区域和周围区域（帕尔帕蒂（El Partido）和玛塔索尔（El Matochal））中的M. spretus的生物学反应。还与环境污染问题相关地确定了关键的抗氧化剂酶的活性，例如Cu / Zn-SOD，Mn-SOD，CAT，GR和愈创木酚过氧化物酶。结果表明，由于生态系统中金属的存在而造成的差异会影响金属和金属蛋白（例如MT，Cu / Zn-SOD或Mn-CA）的水平，膜磷脂的分解，代谢途径的扰动，相关进行能量代谢和氧化应激。

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  • 来源
    《Environmental Science & Technology》 |2014年第14期|7747-7755|共9页
  • 作者

    M. A. Garcia-Sevillano; T. Garcia-Barrera; F. Navarro; N. Abril; C. Pueyo; J. Lopez-Barea; J. L. Gomez-Ariza;

  • 作者单位

    Department of Chemistry and Materials Science, Experimental Sciences Faculty, Research Center on Health and Environment (CYSMA), International Campus of Excellence on Agrofood (CEIA3), Huelva University El Carmen Campus, 21007 Huelva, Spain;

    Department of Chemistry and Materials Science, Experimental Sciences Faculty, Research Center on Health and Environment (CYSMA), International Campus of Excellence on Agrofood (CEIA3), Huelva University El Carmen Campus, 21007 Huelva, Spain;

    Department of Environmental Biology and Public Health, Cell Biology Area, Experimental Sciences Faculty, Huelva University El Carmen Campus, 21007 Huelva, Spain;

    Department of Biochemistry and Molecular Biology, International Campus of Excellence on Agrofood (CEIA3), University of Cordoba, Rabanales Campus, 14071 Cordoba, Spain;

    Department of Biochemistry and Molecular Biology, International Campus of Excellence on Agrofood (CEIA3), University of Cordoba, Rabanales Campus, 14071 Cordoba, Spain;

    Department of Biochemistry and Molecular Biology, International Campus of Excellence on Agrofood (CEIA3), University of Cordoba, Rabanales Campus, 14071 Cordoba, Spain;

    Department of Chemistry and Materials Science, Experimental Sciences Faculty, Research Center on Health and Environment (CYSMA), International Campus of Excellence on Agrofood (CEIA3), Huelva University El Carmen Campus, 21007 Huelva, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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