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首页> 外文期刊>Environmental Science & Technology >Functionality of Aryl Hydrocarbon Receptors (AhR1 and AhR2) of White Sturgeon (Acipenser transmontanus) and Implications for the Risk Assessment of Dioxin-like Compounds
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Functionality of Aryl Hydrocarbon Receptors (AhR1 and AhR2) of White Sturgeon (Acipenser transmontanus) and Implications for the Risk Assessment of Dioxin-like Compounds

机译：白St鱼（Acipenser transmontanus）的芳烃受体（AhR1和AhR2）的功能及其对类似二恶英类化合物的风险评估的意义

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摘要

Worldwide, populations of sturgeons an endangered, and it is hypothesized that anthropogenic chemicals, including dioxin-like compounds (DLCs), might be contributing to the observed declines in populations. DLCs elicit their toxic action through activation of the aryl hydrocarbon receptor (AhR), which is believed to regulate most, if not all, adverse effects associated with exposure to these chemicals. Currently, risk assessment of DLCs in fishes uses toxic equivalency factors (TEFs) developed for the World Health Organization (WHO) that are based on studies of embryo-lethality with salmonids. However, there is a lack of knowledge of the sensitivity of sturgeons to DLCs, and it is uncertain whether TEFs developed by the WHO are protective of these fishes. Sturgeons are evolutionarily distinct from salmonids, and the AhRs of sturgeons differ from those of salmonids. Therefore, this study investigated the sensitivity of white sturgeon (Acipenser transmontamus) to DLCs in vitro via the use of luciferase reporter gene assays using COS-7 cells transfected with AhR1 or AhR2 of white sturgeon. Specifically, activation and relative potencies (RePs) of 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD), 2,3,4,7,8-pentachloro- dibenzofuran, 2,3,7,8-tetrachloro-dibenzofuran, 3,3',4,4',5-pentachlorobiphenyl, 3,3',4,4'-tetrachlorobiphenyl, and 2,3,3',4,4'- pentachlorobiphenyl were determined for each AhR. It was demonstrated that white sturgeon expresses AhR1s and AhR2s that are both activated by DLCs with EC_(50) values for 2,3,7,8-TCDD that are lower than those of any other AhR of vertebrates tested to date. Both AhRs of white sturgeon had RePs for polychlorinated dibenzofurans more similar to TEFs for birds, while RePs for polychlorinated biphenyls were most similar to TEFs for fishes. Measured concentrations of select DLCs in tissues of white sturgeon from British Columbia, Canada, were used to calculate toxic equivalents (TEQs) by use of TEFs for fishes used by the WHO and TCDD equivalents (TCDD-EQs) via the use of RePs for AhR2 of white sturgeon as determined by transfected COS- 7 cells. TCDD-EQs calculated for endangered populations of white sturgeon were approximately 10-fold greater than TEQs and were within ranges known to cause adverse effects in other fishes, including other species of sturgeons. Therefore, TEFs used by the WHO might not adequately protect white sturgeon, illuminating the need for additional investigation into the sensitivity of these fish to DLCs.
机译：在世界范围内，st鱼的种群濒临灭绝，据推测，包括二恶英类化合物（DLCs）在内的人为化学物质可能会导致观察到的种群减少。 DLC通过活化芳烃受体（AhR）引发其毒性作用，据信该化合物可调节大多数（即使不是全部）与接触这些化学物质有关的不利影响。目前，鱼类中DLC的风险评估使用为世界卫生组织（WHO）开发的毒性当量因子（TEF），该因子基于鲑鱼类对胚胎致死性的研究。但是，缺乏对of鱼对DLC敏感性的知识，尚不确定WHO所开发的TEF是否能保护这些鱼类。 evolution鱼在进化上与鲑鱼不同，and鱼的AhR与鲑鱼不同。因此，本研究通过使用萤光素酶报告基因检测使用白using鱼AhR1或AhR2转染的COS-7细胞，研究了白st鱼（Acipenser transmontamus）在体外对DLC的敏感性。具体而言，2,3,7,8-四氯-二苯并-对二恶英（TCDD），2,3,4,7,8-五氯-二苯并呋喃，2,3,7,8的活化和相对效力（RePs）对每个AhR确定了-四氯二苯并呋喃，3,3'，4,4'，5-五氯联苯，3,3'，4,4'-四氯联苯和2,3,3'，4,4'-五氯联苯。事实证明，白色st鱼表达的AhR1和AhR2s均被DLC激活，其EC_（50）值为2、3、7、8-TCDD，低于迄今为止测试的任何其他脊椎动物的AhR。白色st鱼的两个AhRs的多氯二苯并呋喃的ReP与鸟类的TEF更加相似，而多氯联苯的ReP与鱼类的TEF最相似。来自加拿大不列颠哥伦比亚省的白st鱼组织中选择的DLC的测定浓度用于计算WHO所用鱼类的TEF和TCDD等效物（TCDD-EQs）的毒性当量（TEQ），而AhR2则使用RePs由转染的COS-7细胞确定的白色st鱼的数量。为濒临灭绝的白色st鱼种群计算的TCDD-EQs大约是TEQs的10倍，并且在已知会对其他鱼类（包括其他种类的urge鱼）造成不利影响的范围内。因此，WHO使用的TEF可能无法充分保护白st鱼，这说明有必要进一步调查这些鱼对DLC的敏感性。

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  • 来源
    《Environmental Science & Technology》 |2014年第14期|8219-8226|共8页
  • 作者

    Jon A. Doering; Reza Farmahin; Steve Wiseman; Sean W. Kennedy; John P. Giesy; Markus Hecker;

  • 作者单位

    Toxicology Graduate Program, University of Saskatchewan, Saskatoon, SK, Canada,Toxicology Centre, University of Saskatchewan, 44 Campus Saskatoon, SK, Canada S7N 5B3;

    Environment Canada, National Wildlife Research Centre, Ottawa, ON, Canada,Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, ON, Canada;

    Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada;

    Environment Canada, National Wildlife Research Centre, Ottawa, ON, Canada,Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, ON, Canada;

    Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada,Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK, Canada,Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong, Kowloon, Hong Kong, SAR, China,School of Biological Sciences, University of Hong Kong, Hong Kong, SAR, China,State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, China;

    Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada,School of the Environment and Sustainability, University of Saskatchewan, Saskatoon, SK, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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