...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >Immobilization of Catalase on Electrospun PVA/PA6-Cu(Ⅱ) Nanofibrous Membrane for the Development of Efficient and Reusable Enzyme Membrane Reactor
【24h】

Immobilization of Catalase on Electrospun PVA/PA6-Cu(Ⅱ) Nanofibrous Membrane for the Development of Efficient and Reusable Enzyme Membrane Reactor

机译：过氧化氢酶在电纺PVA / PA6-Cu（Ⅱ）纳米纤维膜上的固定化，以开发高效可重复使用的酶膜反应器

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, a mat/membrane consisting of overlaid PVA/PA6-Cu(Ⅱ) composite nanofibers was prepared via the electrospinning technique followed by coordination/chelation with Cu(Ⅱ) ions; an enzyme of catalase (CAT) was then immobilized onto the PVA/PA6-Cu(Il) nanofibrous membrane. The amount of immobilized catalase reached a high value of 64 ± 4.6 mg/g, while the kinetic parameters (V_(max) and K_m of enzyme were 3774 μmol/mg·min and 41.13 mM, respectively. Furthermore, the thermal stability and storage stability of immobilized catalase were improved significantly. Thereafter, a plug-flow type of immobilized enzyme membrane reactor (IEMR) was assembled from the PVA/PA6-Cu(Ⅱ)-CAT membrane. With the increase of operational pressure from 0.02 to 0.2 MPa, the flux value of IEMR increased from 0.20 ± 0.02 to 0.76 ± 0.04 L/m~2·min, whereas the conversion ratio of H_2O_2 decreased slightly from 92 ± 2.5% to 87 ± 2.1%. After 5 repeating cycles, the production capacity of IEMR was merely decreased from 0.144 ± 0.006 to 0.102 ± 0.004 mol/m~2·min. These results indicated that the assembled IEMR possessed high productivity and excellent reusability, suggesting that the IEMR based on electrospun PVA/PA6-Cu(Ⅱ) nanofibrous membrane might have great potential for various applications, particularly those related to environmental protection.
机译：本研究通过电纺丝技术制备了由覆盖的PVA / PA6-Cu（Ⅱ）复合纳米纤维组成的垫/膜，然后与Cu（Ⅱ）离子配位/螯合。然后将过氧化氢酶（CAT）酶固定在PVA / PA6-Cu（II）纳米纤维膜上。固定化过氧化氢酶的量高达64±4.6 mg / g，动力学参数（酶的V_（max）和K_m分别为3774μmol/ mg·min和41.13 mM，此外，其热稳定性和贮存性固定化过氧化氢酶的稳定性得到了显着改善，其后，由PVA / PA6-Cu（Ⅱ）-CAT膜组装了塞流式固定化酶膜反应器（IEMR），工作压力从0.02 MPa增加到0.2 MPa IEMR的通量值从0.20±0.02增加到0.76±0.04 L / m〜2·min，而H_2O_2的转化率从92±2.5％降低到87±2.1％，经过5个重复循环，生产能力提高。 IEMR的值仅从0.144±0.006降至0.102±0.004 mol / m〜2·min，表明组装的IEMR具有较高的生产率和良好的可重复使用性，表明基于电纺PVA / PA6-Cu（Ⅱ）的IEMR纳米纤维膜可能具有广阔的应用前景信息，尤其是与环境保护有关的信息。

著录项

  • 来源
    《Environmental Science & Technology》 |2014年第17期|10390-10397|共8页
  • 作者

    Quan Feng; Yong Zhao; Anfang Wei; Changlong Li; Qufu Wei; Hao Fong;

  • 作者单位

    Key Laboratory of Textile Fabric, College of Textiles and Clothing, Anhui Polytechnic University, Wuhu, Anhui 241000, China, Department of Chemistry and Applied Biological Sciences, South Dakota School of Mines and Technology, Rapid City, South Dakota 57701, United States, Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China;

    Department of Chemistry and Applied Biological Sciences, South Dakota School of Mines and Technology, Rapid City, South Dakota 57701, United States;

    Key Laboratory of Textile Fabric, College of Textiles and Clothing, Anhui Polytechnic University, Wuhu, Anhui 241000, China;

    Key Laboratory of Textile Fabric, College of Textiles and Clothing, Anhui Polytechnic University, Wuhu, Anhui 241000, China;

    Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China;

    Department of Chemistry and Applied Biological Sciences, South Dakota School of Mines and Technology, Rapid City, South Dakota 57701, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号