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首页> 外文期刊>Environmental Science & Technology >Changes in the Expression of cyp35a family Genes in the Soil Nematode Caenorhabditis elegans under Controlled Exposure to Chlorpyrifos Using Passive Dosing
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Changes in the Expression of cyp35a family Genes in the Soil Nematode Caenorhabditis elegans under Controlled Exposure to Chlorpyrifos Using Passive Dosing

机译：被动给药对毒死Control的控制下土壤线虫秀丽隐杆线虫中cyp35a家族基因表达的变化

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摘要

In order to use sensitive molecular-level biomarkers for the evaluation of environmental risks, it is necessary to establish a quantitative dose-response relationship. Passive dosing is regarded as a promising new technique for maintaining a constant exposure condition of hydrophobic chemicals in the assay medium. The main goals of the present study were (1) to quantitatively compare gene expression results obtained using the passive dosing method and the conventional spiking method and (2) to investigate changes in gene expression with respect to the free concentration and exposure duration using passive dosing. Chlorpyrifos (CP), which is oxidized by the cytochrome P450 monooxygenases, was selected as a model chemical, and the expression of cytochrome P450 subfamily protein 35A gene series (cyp-35a1- 5) was analyzed by quantitative real-time PCR on soil nematode Caenorhabditis elegans. Whereas the free concentration of CP rapidly decreased and the expression of cyp genes varied with the volume of exposure medium and the test duration when the spiking method was used, the free concentration in the assay medium was stable throughout the experiment when the passive dosing method was used. In addition, the level of gene expression increased with exposure time up to 8 h and with increasing CP concentration. The observed increased gene expression could be explained by increasing body residue concentration of CP with exposure time. In conclusion, quantitative dose-response relationships for gene expression biomarkers could be obtained for highly hydrophobic chemicals when the constant exposure condition is provided and the free concentration is used as the dose-metric.
机译：为了使用敏感的分子水平生物标记物评估环境风险，有必要建立定量的剂量反应关系。被动加液被认为是在测定介质中保持疏水化学物质恒定暴露条件的一种有前途的新技术。本研究的主要目标是（1）定量比较使用被动加料方法和常规加标方法获得的基因表达结果，以及（2）使用被动加料研究基因表达相对于游离浓度和暴露持续时间的变化。选择被细胞色素P450单加氧酶氧化的毒死rif（CP）作为模型化学品，并通过定量实时PCR在土壤线虫上分析细胞色素P450亚家族蛋白35A基因系列（cyp-35a1-5）的表达。秀丽隐杆线虫。当使用加标方法时，CP的自由浓度迅速降低，并且cyp基因的表达随暴露介质的体积和测试持续时间而变化，而当采用被动加药方法时，测定介质中的自由浓度在整个实验中是稳定的用过的。此外，基因表达水平随暴露时间（长达8小时）和CP浓度的增加而增加。观察到的基因表达增加可以解释为CP的残留浓度随暴露时间的增加而增加。总之，当提供恒定的暴露条件并将游离浓度用作剂量度量时，对于高度疏水的化学物质，可以获得基因表达生物标记物的定量剂量反应关系。

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  • 来源
    《Environmental Science & Technology》 |2014年第17期|10475-10481|共7页
  • 作者

    Ji-Yeon Roh; Hwang Lee; Jung-Hwan Kwon;

  • 作者单位

    Division of Environmental Science and Ecological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 136-713, Republic of Korea;

    Division of Environmental Science and Ecological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 136-713, Republic of Korea;

    Division of Environmental Science and Ecological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 136-713, Republic of Korea;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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