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首页> 外文期刊>Environmental Science & Technology >Proteomic Analysis of Eucalyptus Leaves Unveils Putative Mechanisms Involved in the Plant Response to a Real Condition of Soil Contamination by Multiple Heavy Metals in the Presence or Absence of Mycorrhizal/Rhizobacterial Additives
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Proteomic Analysis of Eucalyptus Leaves Unveils Putative Mechanisms Involved in the Plant Response to a Real Condition of Soil Contamination by Multiple Heavy Metals in the Presence or Absence of Mycorrhizal/Rhizobacterial Additives

机译：桉树叶片的蛋白质组学分析揭示了在存在或不存在菌根/根瘤菌添加剂的情况下，植物对多种重金属污染土壤的真实条件作出反应的推测机制

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摘要

Here we report on the growth, accumulation performances of, and leaf proteomic changes in Eucalyptus camaldulensis plants harvested for different periods of time in an industrial, heavy metals (HMs)-contaminated site in the presence or absence of soil microorganism (AMs/PGPRs) additives. Data were compared to those of control counterparts grown in a neighboring nonpolluted district. Plants harvested in the contaminated areas grew well and accumulated HMs in their leaves. The addition of AMs/PGPRs to the polluted soil determined plant growth and metal accumulation performances that surpassed those observed in the control. Comparative proteomics suggested molecular mechanisms underlying plant adaptation to the HMs challenge. Similarly to what was observed in laboratory-scale investigations on other metal hyperaccumulators but not on HMs-sensitive plants, eucalyptus grown in the contaminated areas showed an over- representation of enzymes involved in photosynthesis and the Calvin cycle. AMs/PGPRs addition to the soil increased the activation of these energetic pathways, suggesting the existence of signaling mechanisms that address the energy/reductive power requirement associated with augmented growth performances. HMs-exposed plants presented an over-representation of antioxidant enzymes, chaperones, and proteins involved in glutathione metabolism. While some antioxidant enzymes/chaperones returned to almost normal expression values in the presence of AMs/PGPRs or in plants exposed to HMs for prolonged periods, proteins guaranteeing elevated glutathione levels were constantly over-represented. These data suggest that glutathione (and related phytochelatins) could act as key molecules for ensuring the effective formation of HMs-chelating complexes that are possibly responsible for the observed plant tolerance to metal stresses. Overall, these results suggest potential genetic traits for further selection of phytoremediating plants based on dedicated cloning or breeding programs.
机译：在这里我们报告在有或没有土壤微生物（AMs / PGPRs）的情况下，在工业，重金属（HMs）污染的地点不同时期收获的桉树camaldulensis植物的生长，累积性能和叶片蛋白质组学变化。添加剂。将数据与在邻近无污染地区种植的对照对等物进行比较。在受污染地区收获的植物生长良好，并在其叶片中积累了HM。在受污染的土壤中添加AMs / PGPRs可以使植物的生长和金属积累性能超过对照中观察到的水平。比较蛋白质组学提出了植物适应HMs挑战的分子机制。与在实验室规模的其他金属超富集性研究上观察到的类似，但对HMs敏感的植物却没有，在受污染地区生长的桉树显示出光合作用和加尔文循环所涉及的酶的过量表达。向土壤中添加AMs / PGPRs增强了这些能量途径的激活，表明存在信号传递机制，可以解决与增长的生长性能相关的能量/还原功率需求。暴露于HMs的植物过量表达了参与谷胱甘肽代谢的抗氧化酶，伴侣蛋白和蛋白质。在AMs / PGPRs存在下或长时间暴露于HMs的植物中，一些抗氧化酶/分子伴侣几乎恢复到正常的表达值，而保证谷胱甘肽水平升高的蛋白质始终被过度代表。这些数据表明，谷胱甘肽（和相关的植物螯合剂）可以作为关键分子，以确保有效形成HMs-螯合复合物，这可能是所观察到的植物对金属胁迫的耐受性。总体而言，这些结果表明了基于专门的克隆或育种程序进一步选择植物修复植物的潜在遗传特性。

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  • 来源
    《Environmental Science & Technology》 |2014年第19期|11487-11496|共10页
  • 作者

    Carmine Guarino; Barbara Conte; Valentina Spada; Simona Arena; Rosaria Sciarrillo; Andrea Scaloni;

  • 作者单位

    Department of Sciences and Technologies, University of Sannio, 82100 Benevento, Italy;

    Department of Sciences and Technologies, University of Sannio, 82100 Benevento, Italy;

    Department of Sciences and Technologies, University of Sannio, 82100 Benevento, Italy;

    Proteomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147 Naples, Italy;

    Department of Sciences and Technologies, University of Sannio, 82100 Benevento, Italy;

    Proteomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147 Naples, Italy;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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