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首页> 外文期刊>Environmental Science & Technology >Indium and Indium Tin Oxide Induce Endoplasmic Reticulum Stress and Oxidative Stress in Zebrafish (Danio rerio)
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Indium and Indium Tin Oxide Induce Endoplasmic Reticulum Stress and Oxidative Stress in Zebrafish (Danio rerio)

机译：铟和氧化铟锡在斑马鱼中引起内质网应激和氧化应激

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摘要

Indium and indium tin oxide (ITO) are extensively used in electronic technologies. They may be introduced into the environment during production, use, and leaching from electronic devices at the end of their life. At present, surprisingly little is known about potential ecotoxico-logical implications of indium contamination. Here, molecular effects of indium nitrate (In(NO_3)_3) and ITO nanopartides were investigated in vitro in zebrafish liver cells (ZFL) cells and in zebrafish embryos and novel insights into their molecular effects are provided. In(NO_3)_3 led to induction of endoplasmic reticulum (ER) stress response, induction of reactive oxygen species (ROS) and induction of transcripts of pro-apoptotic genes and TNF-α in vitro at a concentration of 247 μg/L. In(NO_3)_3 induced the ER stress key gene BiP at mRNA and protein level, as well as atf6, which ultimately led to induction of the important pro-apoptotic marker gene chop. The activity of In(NO_3)_3 on ER stress induction was much stronger than that of ITO, which is explained by differences in soluble free indium ion concentrations. The effect was also stronger in ZFL cells than in zebrafish embryos. Our study provides first evidence of ER stress and oxidative stress induction by In(NO_3)_3 and ITO indicating a critical toxicological profile that needs further investigation.
机译：铟和铟锡氧化物（ITO）广泛用于电子技术中。它们可能会在生产，使用和使用寿命终止时从电子设备中浸入环境中。目前，对铟污染的潜在生态毒理学影响知之甚少。在这里，在斑马鱼肝细胞（ZFL）细胞和斑马鱼胚胎中体外研究了硝酸铟（In（NO_3）_3）和ITO纳米粒子的分子作用，并提供了对其分子作用的新见解。 In（NO_3）_3在体外以247μg/ L的浓度诱导内质网（ER）应激反应，诱导活性氧（ROS）并诱导促凋亡基因和TNF-α转录本。 In（NO_3）_3在mRNA和蛋白质水平以及atf6诱导了ER应激关键基因BiP，最终诱导了重要的促凋亡标记物基因斩波。 In（NO_3）_3对ER应力诱导的活性比ITO强得多，这可以通过可溶性游离铟离子浓度的差异来解释。 ZFL细胞中的作用也比斑马鱼胚胎中的作用更强。我们的研究提供了由In（NO_3）_3和ITO诱导的ER应激和氧化应激诱导的第一个证据，表明需要进一步研究的关键毒理学特征。

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  • 来源
    《Environmental Science & Technology》 |2014年第19期|11679-11687|共9页
  • 作者

    Nadja Rebecca Brun; Verena Christen; Gerhard Furrer; Karl Fent;

  • 作者单位

    University of Applied Sciences and Arts Northwestern Switzerland, School of Life Sciences, Gruendenstrasse 40, CH-4132 Muttenz, Switzerland, Institute of Biogeochemistry and Pollutant Dynamics, ETH Zuerich, Universitaetsstrasse 16, CH-8092 Zuerich, Switzerland;

    University of Applied Sciences and Arts Northwestern Switzerland, School of Life Sciences, Gruendenstrasse 40, CH-4132 Muttenz, Switzerland;

    Institute of Biogeochemistry and Pollutant Dynamics, ETH Zuerich, Universitaetsstrasse 16, CH-8092 Zuerich, Switzerland;

    University of Applied Sciences and Arts Northwestern Switzerland, School of Life Sciences, Gruendenstrasse 40, CH-4132 Muttenz, Switzerland, Institute of Biogeochemistry and Pollutant Dynamics, ETH Zuerich, Universitaetsstrasse 16, CH-8092 Zuerich, Switzerland;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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