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首页> 外文期刊>Environmental Science & Technology >In Vitro Interaction of Emerging Contaminants with the Cytochrome P450 System of Mediterranean Deep-Sea Fish
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In Vitro Interaction of Emerging Contaminants with the Cytochrome P450 System of Mediterranean Deep-Sea Fish

机译：新兴污染物与地中海深海鱼类细胞色素P450系统的体外相互作用

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摘要

The interactions of emerging contaminants with the xenobiotic and endogenous metabolizing system of deep-sea fish were compared. The drags didofenac, fluoxetine, and gemfibrozil belong to different pharmaceutical classes with diverse mechanistic actions, and the personal care products tridosan, galaxolide, and nonylphenol are representative of antibacterial agents, nitro-musks, and surfactants, respectively. The fish compared are representative of the middle and lower slope of deep-sea habitats. The species were adults of Trachyrynchus scabrus, Mora moro, Catactix laticeps, and Alepocehalus rostratus. The hepatic metabolic system studied were the activities associated with several cytochrome P450 isoforms (CYPs): 7-ethoxyresorufin-O-deethylase (EROD), benzylozy-4-[trifiuoromethyl]-coumarin-O-debenzyloxylase (BFCOD), and 7-ethoxycoumarin-O-deethylase (ECOD). Results showed differences in baseline activities and sensitivity to chemicals which were species, chemical, and pathway dependent T. scabrous was the most sensitive species to chemical interactions with the xenobiotic and endogenous metabolizing (EROD and BFCOD) systems, especially in the case of didofenac interference with BFCOD activity (IC_(50)= 15.7 ± 2.2 μM). Moreover, T. scabrous and A rostratus possessed high basal ECOD activity, and this was greatly affected by in vitro exposure to diclofenac in T. scabrous also (IC_(50)= 6.86 ± 1.4 μM). These results highlight the sensitivity of marine fish to emerging contaminants and propose T, scabrous (middle slope) and A. rostratus (lower slope) as sentinels and the inclusion of ECOD activity as a sensitive biomarker to these exposures.
机译：比较了新兴污染物与深海鱼类异种和内源性代谢系统之间的相互作用。药芬多芬，氟西汀和吉非贝齐分别属于具有不同机械作用的不同药物类别，个人护理产品tridosan，galaxolide和壬基酚分别代表抗菌剂，硝基麝香和表面活性剂。比较的鱼类代表了深海生境的中低坡度。该物种是成年的Trachyrynchus scabrus，Mora moro，Catactix laticeps和Alepocehalus rostratus的成虫。研究的肝代谢系统是与几种细胞色素P450亚型（CYPs）相关的活性：7-乙氧基间苯二酚-O-脱乙基酶（EROD），苄基-4- [三氟甲基]-香豆素-O-脱苄基羟化酶（BFCOD）和7-乙氧基香豆素-O-脱乙基酶（ECOD）。结果表明，基线活动和对化学物质的敏感性存在差异，这些化学物质是物种，化学物质和途径依赖的T. scabrous是对与异种生物和内源代谢（EROD和BFCOD）系统化​​学相互作用最敏感的物种，尤其是在双芬诺酸干扰的情况下具有BFCOD活性（IC_（50）= 15.7±2.2μM）。此外，T。s。cabrous和A. rostratus具有较高的基础ECOD活性，这也受到T. s.cabrous中双氯芬酸体外暴露的极大影响（IC_（50）= 6.86±1.4μM）。这些结果突出了海水鱼对新兴污染物的敏感性，并提出将T，粗糙（中坡）和A. rostratus（低坡）作为前哨，并将ECOD活性作为对这些暴露的敏感生物标志物。

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  • 来源
    《Environmental Science & Technology》 |2014年第20期|12327-12335|共9页
  • 作者

    Carla Ribalta; Montserrat Sole;

  • 作者单位

    Institute of Marine Sdences (ICM-CSIC), Pg. Maritim de la Barceloneta 37-49, 08003 Barcelona, Spain;

    Institute of Marine Sdences (ICM-CSIC), Pg. Maritim de la Barceloneta 37-49, 08003 Barcelona, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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