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首页> 外文期刊>Environmental Science & Technology >Comment on 'Determining the Ecological Impacts of Organic Contaminants in Biosolids Using a High-Throughput Colorimetric Denitrification Assay: A Case Study with Antimicrobial Agents'
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Comment on 'Determining the Ecological Impacts of Organic Contaminants in Biosolids Using a High-Throughput Colorimetric Denitrification Assay: A Case Study with Antimicrobial Agents'

机译：评论“使用高通量比色反硝化测定来确定生物固体中有机污染物的生态影响：以抗菌剂为例的研究”

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摘要

The paper by Holzem et al. reports a colorimetric denitrification assay for determining the ecological impacts of organic contaminants present in biosolids. The main shortcoming of this study is that no biosolids was used. The authors simply used a suspension of lab-grown P. denitrificans bacteria in minimal medium, spiked with the selected antimicrobial agents (e.g., triclosan and triclocarban), and quantified the changes in the denitrification rate, gene expression and cell viability. The results were then extrapolated to biosolids, and the assay was suggested as "an attractive alternative for meeting the initial testing regulatory framework for the Federal Insecticide, Fungicide, and Rodenticide Act, and recommended for the Toxic Substances Control Act, in determining the ecotoxicity of biosolids-derived emerging contaminants''. In order to develop a reliable method for biosolids and to determine the ecological impacts of organic contaminants in biosolids, the experimental design must include testing and verification with biosolids. Application rate of biosolids, exposure times, biosolids and soil characteristics, environmental factors, and more importantly biodegra-dation and transport mechanisms will ultimately determine the toxicity caused by the organic contaminants in biosolids. Therefore, the way the paper is written (e.g., title, graphical abstract that shows a picture of a truck land applying biosolids, introduction, etc.) fails to accurately represent the scope of the actual work that was carried out.
机译：Holzem等人的论文。报告了比色反硝化测定法，用于确定生物固体中有机污染物的生态影响。这项研究的主要缺点是没有使用生物固体。作者只是简单地使用实验室生长的反硝化杆菌细菌的悬浮液在基本培养基中悬浮，掺入所选的抗菌剂（如三氯生和三氯卡班），然后量化反硝化率，基因表达和细胞活力的变化。然后将结果推算到生物固体中，并建议该测定法是“满足联邦杀虫剂，杀菌剂和灭鼠剂法的初始测试监管框架的有吸引力的替代方法，并建议通过有毒物质控制法来确定草甘膦的生态毒性。为了开发一种可靠的生物固体方法并确定生物固体中有机污染物的生态影响，实验设计必须包括对生物固体的测试和验证。生物固体的施用率，暴露时间，生物固体和土壤特性，环境因素以及更重要的生物降解和运输机制最终将决定由生物固体中有机污染物引起的毒性。因此，本文的撰写方式（例如，标题，显示卡车图片的图形摘要）施用生物固体，引入土地等的土地）无法准确代表实际工作的范围。

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  • 来源
    《Environmental Science & Technology》 |2014年第20期|12469-12469|共1页
  • 作者

    Banu OErmeci;

  • 作者单位

    Department of Civil and Environmental Engineering, Carleton University, 1125 Colonel By Drive, Ottawa Ontario K1S 5B6, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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