...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >The Regulation of Copper Stress Response Genes in the Polychaete Nereis diversicolor during prolonged Extreme Copper Contamination
【24h】

The Regulation of Copper Stress Response Genes in the Polychaete Nereis diversicolor during prolonged Extreme Copper Contamination

机译：长期极端铜污染过程中多斑沙鸟铜胁迫响应基因的调控

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Polychaetes are frequented in toxicological studies, one reason being that some members occupy shallow burrows in sediments and are maximally exposed to the contaminants that accumulate within them. We have been studying one population of the polychaete Nereis (Hediste) diversicolor exhibiting inheritable tolerance to extreme copper contamination in estuarine sediment. Using transcriptome sequencing data we have identified a suite of genes with putative roles in metal detoxification and tolerance, and measured their regulation. Copper tolerant individuals display significantly different gene expression profiles compared to animals from a nearby population living without remarkable copper levels. Gene transcripts encoding principle copper homeostasis proteins including membrane copper ion transporters, copper ion chaperones and putative metallothionein-like proteins were significantly more abundant in tolerant animals occupying contaminated sediment. In contrast, those encoding antioxidants and cellular repair pathways were unchanged. Nontolerant animals living in contaminated sediment showed no difference in copper homeostasis-related gene expression but did have significantly elevated levels of mRNAs encoding Glutathione Peroxidase enzymes. This study represents the first use of functional genomics to investigate the copper tolerance trait in this species and provides insight into the mechanism used by these individuals to survive and flourish in conditions which are lethal to their conspecifics.
机译：毒cha在毒理学研究中很常见，其原因之一是某些成员在沉积物中占据了较浅的洞穴，并且最大程度地暴露于其中积累的污染物中。我们一直在研究河口沉积物对极端铜污染具有可遗传耐受性的多斑杂色夜蛾（Hediste）。使用转录组测序数据，我们鉴定了一组在金属排毒和耐受性中具有假定作用的基因，并测量了它们的调控。铜耐受性个体与附近没有明显铜水平生活的动物相比，显示出显着不同的基因表达谱。编码主要的铜稳态蛋白的基因转录本，包括膜铜离子转运蛋白，铜离子伴侣蛋白和假定的金属硫蛋白样蛋白，在被污染的沉积物所耐受的动物中明显丰富。相比之下，编码抗氧化剂和细胞修复途径的那些未改变。生活在受污染的沉积物中的非耐受性动物的铜稳态相关基因表达没有差异，但编码谷胱甘肽过氧化物酶的mRNA的水平却显着升高。这项研究代表了功能基因组学首次用于调查该物种的铜耐受性特征，并提供了洞察力，使这些个体能够在对它们的种属致命的条件下生存和繁衍。

著录项

  • 来源
    《Environmental Science & Technology》 |2014年第22期|13085-13092|共8页
  • 作者

    Jonathan S. McQuillan; Peter Kille; Kate Powell; Tamara S. Galloway;

  • 作者单位

    Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, EX4 4QD, U.K.;

    Cardiff School of Biosciences, Cardiff University, Park Place, Cardiff, CF10 3US, U.K.;

    Cardiff School of Biosciences, Cardiff University, Park Place, Cardiff, CF10 3US, U.K.;

    Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, EX4 4QD, U.K.;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号