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首页> 外文期刊>Environmental Science & Technology >Methanospirillum Respiratory mRNA Biomarkers Correlate with Hydrogenotrophic Methanogenesis Rate during Growth and Competition for Hydrogen in an Organochlorine-Respiring Mixed Culture
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Methanospirillum Respiratory mRNA Biomarkers Correlate with Hydrogenotrophic Methanogenesis Rate during Growth and Competition for Hydrogen in an Organochlorine-Respiring Mixed Culture

机译：甲基螺菌呼吸mRNA生物标志物与在有机氯呼吸混合培养物中生长和氢竞争期间的氢营养甲烷生成速率相关。

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摘要

Molecular biomarkers hold promise for inferring rates of key metabolic activities in complex microbial systems. However, few studies have assessed biomarker levels for simultaneously occurring (and potentially competing) respirations. In this study, methanogenesis biomarkers for Methanospirillum hungatei were developed, tested, and compared to Dehalococcoides mccartyi biomarkers in a well-characterized mixed culture. Proteomic analyses of mixed culture samples (n = 4) confirmed expression of many M. hungatei methanogenesis enzymes. The mRNAs for two oxidoreductases detected were explored as quantitative biomarkers of hydrogenotrophic methanogenesis: a coenzyme F_(420)-reducing hydrogenase (FrcA) and an iron sulfur protein (MvrD). As shown previously in D. mccartyi, M. hungatei transcript levels correlated linearly with measured (R - 0.97 for FrcA, R = 0.91 for MvrD; n = 7) or calculated respiration rate (R = 0.81 for FrcA, R = 0.62 for MvrD; n = 35) across two orders of magnitude on a log-log scale. The average abundance of MvrD transcripts was consistently two orders of magnitude lower than FrcA, regardless of experimental condition. In experiments where M. hungatei was competing for hydrogen with D, mccartyi, transcripts for the key respiratory hydrogenase HupL were generally less abundant per mL than FrcA and more abundant than MvrD. With no chlorinated electron acceptor added, HupL transcripts fell below both targets. These biomarkers hold promise for the prediction of in situ rates of respiration for these microbes, even when growing in mixed culture and utilizing a shared substrate which has important implications for both engineered and environmental systems. However, the differences in overall biomarker abundances suggest that the strength of any particular mRNA biomarker relies upon empirically established quantitative trends under a range of pertinent conditions.
机译：分子生物标志物有望推断出复杂微生物系统中关键代谢活动的速率。但是，很少有研究评估同时发生（和潜在竞争）呼吸的生物标志物水平。在这项研究中，在特征明确的混合培养物中，开发，测试并测试了饥饿感甲基甲烷螺旋菌的产甲烷生物标记物，并将其与麦卡氏脱卤球菌生物标记物进行了比较。混合培养物样本（n = 4）的蛋白质组学分析证实了许多匈牙利莫桑比克产甲烷酶的表达。探索了检测到的两种氧化还原酶的mRNA，作为氢营养型甲烷生成的定量生物标记：辅酶F_（420）-还原加氢酶（FrcA）和铁硫蛋白（MvrD）。如先前在D. mccartyi中所示，M. Hangatei转录水平与测量值（FrcA R-0.97，MvrD R = 0.91; n = 7）或计算出的呼吸速率（FrcA R = 0.81，MvrD R = 0.62）线性相关； n = 35），以对数-对数刻度跨越两个数量级。无论实验条件如何，MvrD转录本的平均丰度始终比FrcA低两个数量级。在实验中，匈牙利华氏菌与D，麦卡迪竞争氢，关键呼吸道氢化酶HupL的转录本通常每毫升比FrcA少，但比MvrD丰富。在不添加氯化电子受体的情况下，HupL转录本低于两个目标。这些生物标记物有望预测这些微生物的原位呼吸速率，即使在混合培养中生长并利用对工程和环境系统都具有重要意义的共享底物时也是如此。但是，总体生物标志物丰度的差异表明，任何特定的mRNA生物标志物的强度都取决于在一系列相关条件下凭经验建立的定量趋势。

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  • 来源
    《Environmental Science & Technology》 |2013年第1期|372-381|共10页
  • 作者

    Annette R. Rowe; Cresten B. Mansfeldt; Gretchen L. Heavner; Ruth E. Richardson;

  • 作者单位

    Field of Microbiology, Cornell University, Ithaca, New York, United States,Department of Earth Sciences, SHS 562, University of Southern California, Los Angeles California, 90089-0740;

    Department of Civil and Environmental Engineering, Cornell University, Ithaca, New York, United States;

    Department of Civil and Environmental Engineering, Cornell University, Ithaca, New York, United States;

    Department of Civil and Environmental Engineering, Cornell University, Ithaca, New York, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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