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首页> 外文期刊>Environmental Science & Technology >Toxicity of Functionalized Single-Walled Carbon Nanotubes on Soil Microbial Communities: Implications for Nutrient Cycling in Soil
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Toxicity of Functionalized Single-Walled Carbon Nanotubes on Soil Microbial Communities: Implications for Nutrient Cycling in Soil

机译：功能化的单壁碳纳米管对土壤微生物群落的毒性：对土壤中营养循环的影响

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摘要

Culture-dependent and -independent methods were employed to determine the impact of carboxyl-functionalized single-walled carbon nanotubes (SWNTs) on fungal and bacterial soil microbial communities. Soil samples were exposed to 0 (control), 250, and 500 μg of SWNTs per gram of soil. Aliquots of soil were sampled for up to 14 days for culture-dependent analyses, namely, plate count agar and bacterial community level physiological profiles, and culture-independent analyses, namely, quantitative real-time polymerase chain reaction (qPCR), mutliplex-terminal restriction fragment length polymorphism (M-TRFLP), and clone libraries. Results from culture-independent and -dependent methods show that the bacterial soil community is transiently affected by the presence of SWNTs. The major impact of SWNTs on bacterial community was observed after 3 days of exposure, but the bacterial community completely recovered after 14 days. However, no recovery of the fungal community was observed for the duration of the experiment. Physiological and DNA microbial community analyses suggest that fungi and bacteria involved in carbon and phosphorus biogeochemical cycles can be adversely affected by the presence of SWNTs. This study suggests that high concentrations of SWNTs can have widely varying effects on microbial communities and biogeochemical cycling of nutrients in soils.
机译：采用依赖于文化的方法来确定羧基官能化的单壁碳纳米管（SWNTs）对真菌和细菌土壤微生物群落的影响。土壤样品暴露于每克土壤0（对照），250和500μgSWNTs。将等分试样的土壤采样长达14天，以进行培养依赖性分析，即平板计数琼脂和细菌群落水平的生理概况，以及培养无关分析，即定量实时聚合酶链反应（qPCR），多链末端限制性片段长度多态性（M-TRFLP）和克隆文库。不依赖培养物的方法的结果表明，SWNTs的存在会短暂影响细菌土壤群落。暴露3天后观察到SWNT对细菌群落的主要影响，但14天后细菌群落完全恢复。但是，在实验过程中未观察到真菌群落的恢复。生理和DNA微生物群落分析表明，参与碳和磷生物地球化学循环的真菌和细菌会受到SWNTs的不利影响。这项研究表明，高浓度的单壁碳纳米管对微生物群落和土壤养分的生物地球化学循环具有广泛的影响。

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  • 来源
    《Environmental Science & Technology》 |2013年第1期|625-633|共9页
  • 作者

    Debora F. Rodrigues; Deb P. Jaisi; Menachem Elimelech;

  • 作者单位

    Department of Civil and Environmental Engineering, University of Houston, Houston, Texas 77204-5003, United States,Department of Chemical and Environmental Engineering, Yale University, New Haven, Connecticut 06520-8286, United States;

    Department of Plant and Soil Sciences, University of Delaware, Newark, Delaware 19716, United States,Department of Chemical and Environmental Engineering, Yale University, New Haven, Connecticut 06520-8286, United States;

    Department of Chemical and Environmental Engineering, Yale University, New Haven, Connecticut 06520-8286, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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