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首页> 外文期刊>Environmental Science & Technology >In Situ Metal Imaging and Zn Ligand-Speciation in a Soil-Dwelling Sentinel: Complementary Electron Microprobe and Synchrotron Microbeam X-ray Analyses
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In Situ Metal Imaging and Zn Ligand-Speciation in a Soil-Dwelling Sentinel: Complementary Electron Microprobe and Synchrotron Microbeam X-ray Analyses

机译：土壤居住前哨中的原位金属成像和锌配体形成：互补电子微探针和同步加速器微束X射线分析

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摘要

Understanding the relationships between accumulated metal speciation in cells and tissues of ecologically significant taxa such as earthworms will improve risk assessments. Synchrotron-based μ-focus X-ray spectroscopy was used to detect, localize, and determine ligand-speciation of Zn and Pb in thin sections of two epigeic earthworm species collected from a Pb/Zn-mine soil. The findings indicated that Zn and Pb partition predominantly as typical hard acids (i.e., strong affinities for O-donors) within liverlike chloragocytes. Moreover, Zn speciation was very similar in the chloragog and intestinal epithelia but differed subtly in the kidneylike nephridial tubules; neither Zn nor Pb was detectable in the ventral nerve cord. High resolution X-ray mapping of high pressure-frozen, ultrathin, freeze-substituted sections in a transmission electron microscope (TEM), combined with conventional TEM structural analysis, identified a new cell type packed with highly organized rough endoplasmic reticulum and containing deposits of Cd (codistributed with S); there was no evidence that these cells are major depositories of Zn or Pb. These data may be used in a systems biology approach to assist in the interpretation of metal-evoked perturbations in whole-worm transcriptome and metabolome profiles.
机译：了解具有重要生态意义的分类单元（例如worm）的细胞和组织中积累的金属形态之间的关系将改善风险评估。基于同步加速器的μ聚焦X射线光谱法可用于检测，定位和确定从铅/锌矿土壤中收集的两种表层earth物种的薄片中锌和铅的配体形态。这些发现表明，Zn和Pb主要作为典型的硬酸（即，对O-供体的强亲和力）在肝样氯细胞中分配。此外，锌的形态在叶绿素和肠上皮中非常相似，但在肾样肾小管中却细微地不同。在腹神经索中均未检测到锌和铅。在透射电子显微镜（TEM）中对高压冷冻，超薄，冷冻取代的切片进行高分辨率X射线映射，并结合传统的TEM结构分析，确定了一种新的细胞类型，其中充满了高度组织的粗糙内质网并包含Cd（与S共分布）；没有证据表明这些细胞是Zn或Pb的主要沉积物。这些数据可用于系统生物学方法中，以帮助解释全蠕虫转录组和代谢组谱中的金属诱发的扰动。

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  • 来源
    《Environmental Science & Technology》 |2013年第2期|1073-1081|共9页
  • 作者

    A. J. Morgan; J. F. W. Mosselmans; J. M. Charnock; A. Bennett; C. Winters; M. OReilly; P. Fisher; J. Andre; M. Turner; P. Gunning; P. Kille;

  • 作者单位

    Cardiff School of Biosciences, Cardiff University, Park Place, Cardiff CF10 3US, Wales, U.K.;

    Diamond Light Source Ltd., Harwell Science and Innovation Campus, Didcot, U.K.;

    School of Earth, Atmospheric and Environmental Sciences, University of Manchester, Williamson Building, Oxford Road,Manchester, M13 9PL;

    Science & Technology Facilities Council, Daresbury Laboratory, Daresbury Science & Innovation Campus, Warrington WA4 4AD,Cheshire, U.K.;

    Cardiff School of Biosciences, Cardiff University, Park Place, Cardiff CF10 3US, Wales, U.K.;

    Cardiff School of Biosciences, Cardiff University, Park Place, Cardiff CF10 3US, Wales, U.K.;

    SchooI of Earth & Ocean Sciences, Cardiff University, Main Building, Park Place, Cardiff CF10 3AT, U.K.;

    Cardiff School of Biosciences, Cardiff University, Park Place, Cardiff CF10 3US, Wales, U.K.;

    Cardiff School of Biosciences, Cardiff University, Park Place, Cardiff CF10 3US, Wales, U.K.;

    Smith & Nephew, Heslington, York Science Park, York YO10 5DF, U.K.;

    Cardiff School of Biosciences, Cardiff University, Park Place, Cardiff CF10 3US, Wales, U.K.;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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