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首页> 外文期刊>Environmental Science & Technology >Effects of Proteoliposome Composition and Draw Solution Types on Separation Performance of Aquaporin-Based Proteoliposomes: Implications for Seawater Desalination Using Aquaporin-Based Biomimetic Membranes
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Effects of Proteoliposome Composition and Draw Solution Types on Separation Performance of Aquaporin-Based Proteoliposomes: Implications for Seawater Desalination Using Aquaporin-Based Biomimetic Membranes

机译：蛋白脂质体组成和汲取溶液类型对基于水通道蛋白的脂质体分离性能的影响：使用基于水通道蛋白的仿生膜对海水淡化的影响。

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摘要

Aquaporins are a large family of water transport proteins in cell membranes. Their high water permeability and solute rejection make them potential building blocks for high-performance biomimetic membranes for desalination. In the current study, proteoliposomes were prepared using AquaporinZ from Escherichia coli cells, and their separation properties were characterized by stopped-flow measurements. The current study systematically investigated the effect of proteoliposome composition (lipid type, protein-to-lipid ratio (PLR), and the addition of cholesterol) on water permeability and NaCI retention. Among the various lipids investigated, 1,2-dioleoyl-Stt-gIycero-3-phosphocholine (DOPC)-based proteoliposomes were found to have excellent osmotic water permeability and NaCI reflection coefficient values. Increasing the PLR of DOPC proteoliposomes up to 1:200 increased their osmotic water permeability. However, further increase in the PLR reduced the osmotic water permeability probably due to the occurrence of defects in the proteoliposomes, whereas the addition of cholesterol improved their osmotic water permeation likely due to defects sealing. The current study also investigated the effect of major dissolved ions in seawater (e.g., Mg~(2+) and SO_4~(2-)) on the stability of proteoliposomes, and design criteria for aquaporin-based biomimetic membranes are proposed in the context of desalination.
机译：水通道蛋白是细胞膜中水转运蛋白的一大家族。它们的高透水性和溶质排斥性使其成为高性能仿生膜脱盐的潜在基础。在当前的研究中，使用AquaporinZ从大肠杆菌细胞中制备蛋白脂质体，并通过停止流测量来表征其分离特性。当前的研究系统地研究了蛋白脂质体组成（脂质类型，蛋白质与脂质之比（PLR）和胆固醇的添加）对水渗透性和NaCl保留的影响。在研究的各种脂质中，发现基于1,2-二油酰基-Stt-甘油-3-磷酸胆碱（DOPC）的蛋白脂质体具有优异的渗透水渗透性和NaCl反射系数值。将DOPC蛋白脂质体的PLR增加至1：200，可增加其渗透水渗透性。但是，PLR的进一步增加可能是由于蛋白脂质体中缺陷的出现而降低了渗透水的渗透性，而胆固醇的添加可能是由于缺陷密封而改善了其渗透水的渗透性。当前的研究还研究了海水中主要溶解离子（例如Mg〜（2+）和SO_4〜（2-））对脂质体稳定性的影响，并提出了基于水通道蛋白的仿生膜设计标准。脱盐。

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  • 来源
    《Environmental Science & Technology》 |2013年第3期|1496-1503|共8页
  • 作者

    Yang Zhao; Ardcharaporn Vararattanavech; Xuesong Li; Claus HelixNielsen; Thomas Vissing; Jaume Torres; Rong Wang; Anthony G. Fane; Chuyang Y. Tang;

  • 作者单位

    Singapore Membrane Technology Centre,Nanyang Technological University, Singapore 639798,School of Civil and Environmental Engineering,Nanyang Technological University, Singapore 639798;

    Singapore Membrane Technology Centre,Nanyang Technological University, Singapore 639798,School of Biological Sciences, Nanyang Technological University, Singapore 639798;

    Singapore Membrane Technology Centre,Nanyang Technological University, Singapore 639798,School of Civil and Environmental Engineering,Nanyang Technological University, Singapore 639798;

    Aquaporin A/S, Copenhagen Bio Science Park (COBIS), DK2200, Copenhagen, Denmark,DTU Physics, Technical University of Denmark, DK2800 Kgs. Lyngby, Denmark;

    Aquaporin A/S, Copenhagen Bio Science Park (COBIS), DK2200, Copenhagen, Denmark;

    Singapore Membrane Technology Centre,Nanyang Technological University, Singapore 639798,School of Biological Sciences, Nanyang Technological University, Singapore 639798;

    Singapore Membrane Technology Centre,Nanyang Technological University, Singapore 639798,School of Civil and Environmental Engineering,Nanyang Technological University, Singapore 639798;

    Singapore Membrane Technology Centre,Nanyang Technological University, Singapore 639798,School of Civil and Environmental Engineering,Nanyang Technological University, Singapore 639798;

    Singapore Membrane Technology Centre,Nanyang Technological University, Singapore 639798,Aquaporin A/S, Copenhagen Bio Science Park (COBIS), DK2200, Copenhagen, Denmark;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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