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Compounded Effects of Chlorinated Ethene Inhibition on Ecological Interactions and Population Abundance in a Dehalococcoides -Dehalobacter Coculture

机译:氯化乙烯抑制剂对Dehaloccocoides -Dehalobacter共培养中生态相互作用和种群数量的复合作用

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摘要

The development of rational and effective engineered bioremediation approaches for sites contaminated with chlorinated solvents requires a fundamental understanding of the factors limiting the in situ activity of dehalorespiring bacteria. Frequently, multiple dehalorespiring bacteria are present at contaminated sites, particularly when bioaugmentation is applied. The ecological interactions between different dehalorespiring populations can-along with hydrodynamic and other environmental factors-affect their activity and thus the rates and extent of dehalorespiration. An integrated experimental and modeling approach was used to evaluate the ecological interactions between two hydrogenotrophic, dehalorespiring strains. A dual Monod model of dehalorespiration provided a good fit to the chlorinated ethene concentrations measured in a coculture of Dehalococcoides mccartyi 195 and Dehalobacter restrictus growing on tetrachloroethene (PCE) and excess H_2 in a continuous-flow reactor. Inhibition of dehalorespiration by chlorinated ethenes was previously observed in cultures containing Dehalococcoides or Dehalobacter strains. Therefore, inhibition coefficients were estimated for Dhc. mccartyi 195 and Dhb. restrictus. The inhibition effects of PCE and TCE on VC dechlorination by Dhc mccartyi 195, and of VC on PCE and TCE dechlorination by Dhb. restrictus, were compounded when these strains were grown in coculture, and dehalorespiring population abundance and survival could be accurately predicted only by incorporating these complex interactions into the dual Monod model.
机译:对于被氯化溶剂污染的场所,要开发合理有效的工程生物修复方法,需要对限制除汗呼吸细菌的原位活性的因素有基本的了解。通常,受污染的部位会存在多种脱呼吸细菌,尤其是在进行生物增强时。不同的除呼吸作用的人群之间的生态相互作用会随着流体动力和其他环境因素的影响而影响其活动,进而影响除呼吸作用的速度和程度。综合的实验和建模方法用于评估两个氢营养型,除盐呼吸型菌株之间的生态相互作用。脱汗的双重Monod模型非常适合在连续流动反应器中在四氯乙烯(PCE)和过量H_2上生长的Dehalococcoides mccartyi 195和限制性脱盐杆菌共培养物中测得的氯化乙烯浓度。以前在含有Dehalococcoides或Dehalobacter菌株的培养物中观察到氯化乙烯对脱汗的抑制作用。因此,估计了Dhc的抑制系数。麦卡蒂195和Dhb。限制。 PCE和TCE对Dhc mccartyi 195对VC脱氯的抑制作用,以及VC对Dhb对PCE和TCE脱氯的抑制作用。当这些菌株在共培养物中生长时,限制性内切酶变得更加复杂,只有将这些复杂的相互作用纳入对偶Monod模型中,才能准确预测出脱卤呼吸人口的丰度和存活率。

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  • 来源
    《Environmental Science & Technology》 |2013年第3期|1518-1525|共8页
  • 作者单位

    Swette Center for Environmental Biotechnology, The Biodesign Institute at Arizona State University, Tempe, Arizona 85287-5701, United States;

    Michigan Technological University, Houghton, Michigan 49931, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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