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首页> 外文期刊>Environmental Science & Technology >Monitoring Functions in Managed Microbial Systems by Cytometric Bar Coding
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Monitoring Functions in Managed Microbial Systems by Cytometric Bar Coding

机译：通过流式细胞条形码监测受管微生物系统中的功能

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摘要

Cytometric monitoring of microbial community dynamics can be used to estimate stability of technical microbial processes like biogas production by analysis of segregated cell abundance changes. In this study, structure variations of a biogas community were cytometrically recorded over 9 months and found to be of diagnostic value for process details. The reactor regime was intentionally disturbed with regard to substrate overload or H_2S accumulation. A single-cell based approach called cytometric bar coding 1 (CyBar) for fast identification of reactive subcommunities was used. Functionality of specific subcommunities was uncovered by processing CyBar data with abiotic reactor parameters using Spearman's correlation coefficient. Twenty subcommunities showed a discrete and divergent behavior. For example, a 4-fold substrate overload increased the cell number of two acidogenic index subcommunities to 176 and 193% within three days. Supplementary analyses were done using DNA fingerprinting, cloning, and sequencing. Bioreactor perturbations were shown to create cell abundance changes in subcommunities rather than variations in their phylogenetic composition. The used workflow and macros are ready-to-use tools and allow on-site monitoring and interpretation of variation in microbial community functions within a few hours.
机译：细胞计数监测微生物群落动力学可用于通过分析分离的细胞丰度变化来估算技术性微生物过程（如沼气生产）的稳定性。在这项研究中，在9个月内通过细胞计数技术记录了沼气群落的结构变化，发现该变化对处理细节具有诊断价值。在底物超载或H_2S积累方面，故意扰乱了反应器方案。使用了一种基于单细胞的方法，称为细胞计数条形码1（CyBar），用于快速识别反应性亚社区。通过使用Spearman相关系数使用非生物反应堆参数处理CyBar数据，发现了特定亚社区的功能。 20个亚社区表现出离散和分歧的行为。例如，四倍的底物超负荷在三天内将两个产酸指数亚社区的细胞数增加到176和193％。使用DNA指纹图谱，克隆和测序进行补充分析。研究表明，生物反应器的扰动会在亚群落中造成细胞丰度的变化，而不是其系统发育组成的变化。使用的工作流程和宏是现成的工具，可以在几个小时内对微生物群落功能的变化进行现场监控和解释。

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  • 来源
    《Environmental Science & Technology》 |2013年第3期|1753-1760|共8页
  • 作者

    Christin Koch; Ingo Fetzer; Thomas Schmidt; Hauke Harms; Susann Mueller;

  • 作者单位

    Department of Bioenergy, UFZ - Helmholtz Centre for Environmental Research, Leipzig, Germany;

    Department of Environmental Microbiology, UFZ - Helmholtz Centre for Environmental Research, Leipzig, Germany;

    Department Biochemical Conversion, DBFZ - German Biomass Research Centre, Leipzig, Germany;

    Department of Environmental Microbiology, UFZ - Helmholtz Centre for Environmental Research, Leipzig, Germany;

    Department of Environmental Microbiology, UFZ - Helmholtz Centre for Environmental Research, Leipzig, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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