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首页> 外文期刊>Environmental Science & Technology >Genome-Centric Metatranscriptomics Analysis Reveals the Role of Hydrochar in Anaerobic Digestion of Waste Activated Sludge
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Genome-Centric Metatranscriptomics Analysis Reveals the Role of Hydrochar in Anaerobic Digestion of Waste Activated Sludge

机译:以基因组的MetaTranscriptomics分析揭示了氢乙酸在废物活性污泥的厌氧消化中的作用

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摘要

Anaerobic digestion (AD) of waste activated sludge (WAS) has been widely used, while it poses problems including low methane yield and production rate. Hydrochar is produced by hydrothermal liquefaction of biomass; however, little is known about the role of hydrochar in promoting AD of WAS. The present study showed that hydrochar increased the methane production rate by 30.8% and yield by 31.4% of hydrothermal pretreated dewatered WAS. Hydrochar increased the methane production rate and yield by enhancing the acidification and methanogenesis processes. Genomic-centric metatranscriptomics were used to identify the metabolic activities and transcriptomic response of individual metagenome-assembled genomes that were enriched by hydrochar. Although Methanosarcina sp. FDU0106 had been shown unable to used H_2, it had the complete pathway for the reduction of CO_2 to methane. Syntrophomonas sp. FDU0164 expressed genes for extracellular electron transfer via electrically pili, suggesting that Syntrophomonas sp. FDU0164 and Methanosarcina sp. FDU0106 were exchanging electrons via direct interspecies electron transfer. The expression of pili was decreased, indicating that hydrochar could replace its roles. Additionally, Firmicutes sp. FDU0048, Proteiniphilum sp. FDU0082, and Aminobacterium mobile FDU0089 were related to the degradation of organics, which could be related to the enhanced methane yield.
机译:废物活性污泥(AD)的厌氧消化(AD)已被广泛使用,而造成甲烷产量和生产率的问题。水热液化生物质的水热液化;但是,关于氢乙酰件在促进AD的促进时几乎是众所周知的。本研究表明,氢乙烷产量将甲烷产率提高30.8%,产率为31.4%的水热预处理脱水。通过增强酸化和甲状腺发生方法,水解增加甲烷的生产率和产率。以基因组形状的偏扫描组织术用于鉴定富含氢氧的单个酵母组组装基因组的代谢活性和转录组反应。虽然MethanoSarcina sp。 FDU0106已被显示出不能使用H_2,它具有完整的途径,用于减少CO_2至甲烷。 Syntrophomonas sp。 FDU0164通过电酶表达细胞外电子转移的基因,表明Syntrophomonas sp。 FDU0164和MethanoSarcina SP。 FDU0106通过直接交叉电子传输进行电子交换电子。 Pili的表达减少,表明氢乙酸可以取代其作用。此外,FUMPICUTES SP。 FDU0048,ProteiniPhilum sp。 FDU0082和氨基杆菌FDU0089与有机物的降解有关,这可能与增强的甲烷产率有关。

著录项

  • 来源
    《Environmental Science & Technology》 |2021年第12期|8351-8361|共11页
  • 作者单位

    Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3) Department of Environmental Science and Engineering Fudan University Shanghai 200438 China;

    Department of Biology University of Padova 35121 Padua Italy;

    Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3) Department of Environmental Science and Engineering Fudan University Shanghai 200438 China Bioproducts Science and Engineering Laboratory Washington State University (WSU) Tri-Cities Washington 99354 United States;

    Department of Biology University of Padova 35121 Padua Italy;

    Department of Biology University of Padova 35121 Padua Italy;

    Department of Environmental Engineering Technical University of Denmark 2800 Kongens Lyngby Denmark;

    Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3) Department of Environmental Science and Engineering Fudan University Shanghai 200438 China Shanghai Technical Service Platform for Pollution Control and Resource Utilization of Organic Wastes Shanghai 200438 China Shanghai Institute of Pollution Control and Ecological Security Shanghai 200092 China;

    Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3) Department of Environmental Science and Engineering Fudan University Shanghai 200438 China Shanghai Technical Service Platform for Pollution Control and Resource Utilization of Organic Wastes Shanghai 200438 China Shanghai Institute of Pollution Control and Ecological Security Shanghai 200092 China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    anaerobic digestion; direct interspecies electron transfer; waste activated sludge; metabolic reconstruction; methanogenesis;

    机译:厌氧消化;直接散列电子转移;废物活性污泥;代谢重建;甲烷化;

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