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首页> 外文期刊>Environmental Science & Technology >Anaerobic Neutrophilic Pyrite Oxidation by a Chemolithoautotrophic Nitrate-Reducing Iron(Ⅱ)-Oxidizing Culture Enriched from a Fractured Aquifer
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Anaerobic Neutrophilic Pyrite Oxidation by a Chemolithoautotrophic Nitrate-Reducing Iron(Ⅱ)-Oxidizing Culture Enriched from a Fractured Aquifer

机译：厌氧中性硫化物氧化通过宁静营养的硝酸铁（Ⅱ） - 富含碎屑含水层的氧化培养物

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摘要

Neutrophilic microbial pyrite (FeS_2) oxidation coupled to denitrification is thought to be an important natural nitrate attenuation pathway in nitrate-contaminated aquifers. However, the poor solubility of pyrite raises questions about its bioavailability and the mechanisms underlying its oxidation. Here, we investigated direct microbial pyrite oxidation by a neutrophilic chemolithoautotrophic nitrate-reducing Fe(Ⅱ)-oxidizing culture enriched from a pyrite-rich aquifer. We used pyrite with natural abundance (NA) of Fe isotopes (~(NA)Fe-pyrite) and ~(57)Fe-labeled siderite to evaluate whether the oxidation of the more soluble Fe(Ⅱ)-carbonate (FeCO_3) can indirectly drive abiotic pyrite oxidation. Our results showed that in setups where only pyrite was incubated with bacteria, direct microbial pyrite oxidation contributed ca. 26% to overall nitrate reduction. The rest was attributed to the oxidation of elemental sulfur (S~0), present as a residue from pyrite synthesis. Pyrite oxidation was evidenced in the ~(NA)Fe-pyrite/~(57)Fe-siderite setups by maps of ~(56)FeO and ~(32)S obtained using a combination of SEM with nanoscale secondary ion MS (NanoSIMS), which showed the presence of ~(56)Fe(Ⅲ) (oxyhydr)oxides that could solely originate from ~(56)FeS_2. Based on the fit of a reaction model to the geochemical data and the Fe-isotope distributions from NanoSIMS, we conclude that anaerobic oxidation of pyrite by our neutrophilic enrichment culture was mainly driven by direct enzymatic activity of the cells. The contribution of abiotic pyrite oxidation by Fe~(3+) appeared to be negligible in our experimental setup.
机译：含有脱氧硝化的嗜中性微生物黄铁矿（FES_2）氧化被认为是硝酸盐污染的含水层中的重要天然硝酸盐衰减途径。然而，黄铁矿的溶解度不良引起了关于其生物利用度的问题和其氧化下面的机制。在此，我们通过中性培养的富含培养的硝酸盐（Ⅱ） - 氧化来自富含硫铁矿的含水层的富含氧化培养物的直接微生物硫醇盐氧化。我们使用具有天然丰度（Na）的Fe同位素（〜（Na）Fe-硫铁矿）和〜（57）Fe标记的散晶铁矿石，以评估更可溶的Fe（Ⅱ）碳酸盐（Feco_3）的氧化是否可以间接地驱动非生物硫酸盐氧化。我们的研究结果表明，在设置只与细菌孵育黄铁矿，直接微生物黄铁矿氧化贡献了CA.总整体硝酸盐减少26％。其余的归因于元素硫（S〜0）的氧化，作为来自硫铁矿合成的残余物。通过使用SEM的组合与纳米级次离子MS（纳米键）的组合获得的〜（Na）Fe-Pylite /〜（57）Fe-Siderite设置在〜（Na）Fe-Pylite /〜（57）Fe-Siderite设置中证实了硫酸盐氧化。 ，表明存在〜（56）Fe（Ⅲ）（氧水）氧化物，其可以单独源自〜（56）FES_2。基于反应模型与地球化学数据的拟合和来自纳米粒子的Fe-IsoTope分布，我们得出结论，通过对中性粒料富集培养的苯铁矿厌氧氧化主要是通过细胞的直接酶活性驱动。在我们的实验设置中，Fe〜（3+）的非生物硫酸盐氧化的贡献似乎可以忽略不计。

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  • 来源
    《Environmental Science & Technology》 |2021年第14期|9876-9884|共9页
  • 作者

    Natalia Jakus; Adrian Mellage; Carmen Hoeschen; Markus Maisch; James M. Byrne; Carsten W. Mueller; Peter Grathwohl; Andreas Kappler;

  • 作者单位

    Geomicrobiology Center for Applied Geoscience and Microbial Ecology Center for Applied Geoscience University of Tuebingen Tuebingen D-72076 Germany;

    Hydrogeology Center for Applied Geosciences University of Tuebingen Tuebingen D-72076 Germany;

    Soil Science TUM School of Life Sciences Technical University of Munich Freising-Weihenstephan D-85354 Germany;

    Geomicrobiology Center for Applied Geoscience University of Tuebingen Tuebingen D-72076 Germany;

    Geomicrobiology Center for Applied Geoscience University of Tuebingen Tuebingen D-72076 Germany School of Earth Sciences University of Bristol BS8 1RJ Bristol U.K. (J.M.B.);

    Soil Science TUM School of Life Sciences Technical University of Munich Freising-Weihenstephan D-85354 Germany Department of Geosciences and Natural Resource Management University of Copenhagen DK-1350 Copenhagen Denmark (C.W.M.);

    Hydrogeochemistry Center for Applied Geoscience University of Tuebingen D-72076 Tubingen Germany;

    Geomicrobiology Center for Applied Geoscience University of Tuebingen Tuebingen D-72076 Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    denitrification; siderite oxidation; NRFeOx; anoxic subsurface;

    机译：反硝化;渗透氧化;NRFEOX;缺氧地下;

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