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首页> 外文期刊>Environmental Science & Technology >Arsenic Dissolution from Japanese Paddy Soil by a Dissimilatory Arsenate-Reducing Bacterium Geobacter sp. OR-1
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Arsenic Dissolution from Japanese Paddy Soil by a Dissimilatory Arsenate-Reducing Bacterium Geobacter sp. OR-1

机译：异化还原砷的细菌Geobacter sp。从日本水稻土中溶解砷。或1

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摘要

Dissimilatory As(Ⅴ) (arsenate)-reducing bacteria may play an important role in arsenic release from anoxic sediments in the form of As(Ⅲ) (arsenite). Although respiratory arsenate reductase genes (arrA) closely related to Geobacter species have been frequently detected in arsenic-rich sediments, it is still unclear whether they directly participate in arsenic release, mainly due to lack of pure cultures capable of arsenate reduction. In this study, we isolated a novel dissimilatory arsenate-reducing bacterium, strain OR-1, from Japanese paddy soil, and found that it was phylogenetically closely related to Geobacter pelophilus. OR-1 also utilized soluble Fe(Ⅲ), ferrihydrite, nitrate, and fumarate as electron acceptors. OR-1 catalyzed dissolution of arsenic from arsenate-adsorbed ferrihydrite, while Geobacter metallireducens GS-15 did not. Furthermore, inoculation of washed cells of OR-1 into sterilized paddy soil successfully restored arsenic release. Arsenic K-edge X-ray absorption near-edge structure analysis revealed that strain OR-1 reduced arsenate directly on the soil solid phase. Analysis of putative ArrA sequences from paddy soils suggested that Geobacter-related bacteria, including those closely related to OR-1, play an important role in arsenic release from paddy soils. Our results provide direct evidence for arsenic dissolution by Geobacter species and support the hypothesis that Geobacter species play a significant role in reduction and mobilization of arsenic in flooded soils and anoxic sediments.
机译：异化还原砷（Ⅴ）（砷）的细菌可能以砷（Ⅲ）（亚砷酸盐）的形式从缺氧沉积物中释放砷。尽管经常在富含砷的沉积物中检测到与地球细菌物种密切相关的呼吸道砷还原酶基因（arrA），但仍不清楚它们是否直接参与砷释放，主要是由于缺乏能够还原砷的纯培养物。在这项研究中，我们从日本水稻土中分离出了一种新型的异化砷酸盐还原细菌，即OR-1菌株，发现该菌株与嗜果芽胞杆菌在系统发育上密切相关。 OR-1还利用可溶性Fe（Ⅲ），亚铁酸盐，硝酸盐和富马酸盐作为电子受体。 OR-1催化砷从吸附砷酸盐的三水铁矿中溶解，而Geomicrometallireducens GS-15则没有。此外，将洗涤过的OR-1细胞接种到无菌稻田中可以成功地恢复砷的释放。砷的K边缘X射线吸收近边缘结构分析表明，菌株OR-1直接在土壤固相上还原了砷。从水稻土推定的ArrA序列分析表明，与土壤细菌有关的细菌，包括与OR-1密切相关的细菌，在水稻土中的砷释放中起重要作用。我们的研究结果直接证明了地细菌对砷的溶解，并支持以下假设：地细菌在减少和富集土壤和缺氧沉积物中的砷中起着重要作用。

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  • 来源
    《Environmental Science & Technology》 |2013年第12期|6263-6271|共9页
  • 作者

    Toshihiko Ohtsuka; Noriko Yamaguchi; Tomoyuki Makino; Kazuhiro Sakurai; Kenta Kimura; Keitaro Kudo; Eri Homma; Dian Tao Dong; Seigo Amachi;

  • 作者单位

    Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo-city, Chiba 271-8510, Japan;

    NationaI Institute for Agro-Environmental Sciences, 3-1-3, Kan-nondai, Tsukuba-city, Ibaraki 305-8604, Japan;

    NationaI Institute for Agro-Environmental Sciences, 3-1-3, Kan-nondai, Tsukuba-city, Ibaraki 305-8604, Japan;

    Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo-city, Chiba 271-8510, Japan;

    Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo-city, Chiba 271-8510, Japan;

    Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo-city, Chiba 271-8510, Japan;

    Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo-city, Chiba 271-8510, Japan;

    Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo-city, Chiba 271-8510, Japan;

    Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo-city, Chiba 271-8510, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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