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首页> 外文期刊>Environmental Science & Technology >Carbon and Chlorine Isotope Fractionation During Microbial Degradation of Tetra- and Trichloroethene
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Carbon and Chlorine Isotope Fractionation During Microbial Degradation of Tetra- and Trichloroethene

机译：四氯乙烯和三氯乙烯微生物降解过程中的碳和氯同位素分馏

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摘要

Two-dimensional compound-specific isotope analysis (2D-CSIA), combining stable carbon and chlorine isotopes, holds potential for monitoring of natural attenuation of chlorinated ethenes (CEs) in contaminated soil and groundwater. However, interpretation of 2D-CS1A data sets is challenged by a shortage of experimental Cl isotope enrichment factors. Here, isotope enrichments factors for C and Cl (i.e., ε_c and ε_(cl)) were determined for biodegradation of tetrachloroethene (PCE) and trichloroethene (TCE) using microbial enrichment cultures from a heavily CE-contaminated aquifer. The obtained values were ε_c = -5.6 ± 0.7‰ (95% Cl) and ε_(cl - -2.0 ± 0.5‰ for PCE degradation and ε_c = -8.8 ± 0.2‰ and ε_(cl) = -3.5 ± 0.5‰ for TCE degradation. Combining the values for bom ε_c and ε_(cl) yielded mechanism-diagnostic ε_(cl)/ε_c ratios of 035 ± 0.11 and 037 ± 0.11 for the degradation of PCE and TCE, respectively. Application of the obtained ε_c) and ε_(cl) values to a previously investigated field site gave similar estimates for the fraction of degraded contaminant as in the previous study, but with a reduced uncertainty in assessment of the natural attenuation. Furthermore, 16S rRNA gene clone library analyses were performed on three samples from the PCE degradation experiments. A species closely related to Desulfitobacterium aromaticivorans UKTL dominated the reductive dechlorination process. This study contributes to the development of 2D-CSIA as a tool for evaluating remediation strategies of CEs at contaminated sites.
机译：二维化合物特异性同位素分析（2D-CSIA），结合了稳定的碳和氯同位素，具有监测被污染的土壤和地下水中氯化乙烯（CE）自然衰减的潜力。但是，缺乏实验性Cl同位素富集因子对2D-CS1A数据集的解释提出了挑战。在此，确定了C和Cl的同位素富集因子（即ε_c和ε_（cl）），以利用受严重CE污染的含水层中的微生物富集培养物对四氯乙烯（PCE）和三氯乙烯（TCE）进行生物降解。获得的值为PCE降解的ε_c= -5.6±0.7‰（95％Cl）和ε_（cl--2.0±0.5‰），TCE的ε_c= -8.8±0.2‰和ε_（cl）= -3.5±0.5‰结合bom的ε_c和ε_（cl）的值，得出PCE和TCE的降解机理诊断的ε_（cl）/ε_c比分别为035±0.11和037±0.11。得到的ε_c）和ε_的应用（cl）先前研究现场的值对降解污染物的分数进行了与先前研究相似的估算，但是自然衰减评估的不确定性降低了。此外，对来自PCE降解实验的三个样品进行了16S rRNA基因克隆文库分析。与芳香脱硫杆菌UKTL密切相关的物种主导了还原脱氯过程。这项研究有助于2D-CSIA的开发，作为评估受污染场所的CE修复策略的工具。

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  • 来源
    《Environmental Science & Technology》 |2013年第12期|6449-6456|共8页
  • 作者

    Charline Wiegert; Manolis Mandalakis; Tim Knowles; Paraskevi N. Polymenakou; Christoph Aeppli; Jirina Machackova; Henry Holmstrand; Richard P. Evershed; Richard D. Pancost; OErjan Gustafsson;

  • 作者单位

    Department of Applied Environmental Science (ITM), Stockholm University, 106 91 Stockholm, Sweden;

    Hellenic Centre for Marine Research (HCMR), 71003 Heraklion, Crete, Greece;

    School of Chemistry, University of Bristol, Bristol BS8 1TS, United Kingdom,Mass Spec Analytical Ltd., Building 20F, Golf Course Lane, Bristol, BS34 7RP, England;

    Hellenic Centre for Marine Research (HCMR), 71003 Heraklion, Crete, Greece;

    Department of Applied Environmental Science (ITM), Stockholm University, 106 91 Stockholm, Sweden,Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA;

    AECOM CZ s.r.o, Liberec 460 11, Czech Republic;

    Department of Applied Environmental Science (ITM), Stockholm University, 106 91 Stockholm, Sweden;

    School of Chemistry, University of Bristol, Bristol BS8 1TS, United Kingdom;

    School of Chemistry, University of Bristol, Bristol BS8 1TS, United Kingdom;

    Department of Applied Environmental Science (ITM), Stockholm University, 106 91 Stockholm, Sweden;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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