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首页> 外文期刊>Environmental Science & Technology >Vanadate and Acetate Biostimulation of Contaminated Sediments Decreases Diversity, Selects for Specific Taxa, and Decreases Aqueous V~(5+) Concentration
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Vanadate and Acetate Biostimulation of Contaminated Sediments Decreases Diversity, Selects for Specific Taxa, and Decreases Aqueous V~(5+) Concentration

机译:钒和乙酸对受污染沉积物的生物刺激作用降低多样性,选择特定的分类单元,降低V〜(5+)水溶液的浓度

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摘要

Vanadium is a commercially important metal that is released into the environment by fossil fuel combustion and mining. Despite its prevalence as a contaminant, the potential for vanadium bioremediarion has not been widely studied. Injection of acetate (as a carbon source) directly into an aquifer to biostimulate contaminated sediments in Colorado, United States, resulted in prolonged removal of aqueous vanadium for a period of at least two years. To further investigate this process, we simultaneously added acetate and vanadate (V~(5+)) to columns that were packed with aquifer sediment and inserted into groundwater wells installed on the Colorado River floodplain. This allowed evaluation of the microbial response to amendments in columns that received an influx of natural groundwater. Our results demonstrate the removal of up to 99% of the added V~(5+) (aq) and suggest microbial mediation. Most probable number measurements demonstrate up to a 50-fold increase in numbers of V~(5+)-redudng cells in vanadium-amended columns compared to controls. 16S rRNA gene sequencing indicates decreased diversity and selection for specific taxa in columns that received vanadate compared to those that did not Overall, our results demonstrate that acetate amendment can be an effective strategy for V removal, and that V bioremediation may be a viable technology.
机译:钒是一种商业上重要的金属,通过化石燃料燃烧和开采释放到环境中。尽管其作为污染物盛行,但钒生物修复的潜力尚未得到广泛研究。将乙酸盐(作为碳源)直接注入含水层中以生物刺激受污染的沉积物(美国科罗拉多州),导致钒水溶液的去除时间延长至少两年。为了进一步研究此过程,我们将醋酸盐和钒酸盐(V〜(5+))同时添加到充满含水层沉积物的色谱柱中,并插入安装在科罗拉多河漫滩上的地下水井中。这样可以评估微生物对收到天然地下水涌入的色谱柱中的修正物的反应。我们的结果表明,去除了高达99%的添加的V〜(5+)(水溶液),并建议进行微生物介导。最可能的数量测量结果表明,与对照相比,钒修饰柱中V〜(5+)还原细胞的数量最多增加了50倍。 16S rRNA基因测序表明,与未接受钒酸盐的色谱柱相比,接受钒酸盐的色谱柱中特定分类单元的多样性和选择减少。总体而言,我们的结果表明乙酸盐修饰可以是去除V的有效策略,V生物修复可能是可行的技术。

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  • 来源
    《Environmental Science & Technology》 |2013年第12期|6500-6509|共10页
  • 作者单位

    Department of Environmental Science, Policy, and Management, University of California, Berkeley, California 94720, United States,Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States;

    Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States;

    Department of Geoscience, University of Wisconsin-Madison, Madison, Wisconsin S3706, United States;

    Earth and Planetary Science, University of California, Berkeley, California 94720, United States;

    Earth and Planetary Science, University of California, Berkeley, California 94720, United States,Ecology and Evolution, University of Chicago, Chicago, Illinois 60637, United States;

    Department of Environmental Science, Policy, and Management, University of California, Berkeley, California 94720, United States,Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States,Earth and Planetary Science, University of California, Berkeley, California 94720, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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